Skip to main content
Addgene

We narrowed to 16,985 results for: She

Showing: 11901 - 11950 of 16985 results
  1. pNK189

    Plasmid
    #219743
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens
    Depositor
    Insert
    mutant of fungal hispidin-3-hydroxylase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    D37E, V181I, S323M, M385K
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pNK4169

    Plasmid
    #219744
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris
    Depositor
    Insert
    mutant of fungal hispidin-3-hydroxylase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    D37E, V181I, S323M, M385K
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. B52_puro_gRNA_3418711_KCNB2

    Plasmid
    #197558
    Purpose
    gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)
    Depositor
    Insert
    upstream KCNB2 promoter gRNA (Kcnb2 Rat)
    Use
    Grna with puromycin selection
    Tags
    puromycin resistance cassette under CMV promotion
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pET24TEV-tSHIP1

    Plasmid
    #183770
    Purpose
    Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone
    Depositor
    Insert
    SHIP1 (INPP5D Human)
    Use
    Tags
    6HIS and Tobacco Etch Virus (TEV) Cleavage site
    Expression
    Bacterial
    Mutation
    includes aa1 to aa883 only
    Promoter
    T7 lac
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pET24TEV-tSHIP2

    Plasmid
    #183771
    Purpose
    Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone
    Depositor
    Insert
    SHIP2 (INPPL1 Human)
    Use
    Tags
    6HIS and Tobacco Etch Virus (TEV) Cleavage site
    Expression
    Bacterial
    Mutation
    includes aa18 to aa985 only
    Promoter
    T7 lac
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA T7-Gnat1 Mut-3UTR

    Plasmid
    #184027
    Purpose
    Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA
    Depositor
    Insert
    Gnat1 (Gnat1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    TAG sites in the 3'-UTR are mutated to TGA
    Promoter
    CMV
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pQC V5 mKO2 HuR.DelHNS IRES Puro

    Plasmid
    #110389
    Purpose
    γ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    V5 and mKO2
    Expression
    Mammalian
    Mutation
    HNS (HuR nuclear-cytoplasmic shuttling sequence) …
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. EcNGsC-DelFSH(307-309)-pKK223

    Plasmid
    #131377
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.
    Depositor
    Insert
    EcNGsC MutY chimera DelFSH(307-309)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are from…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. EcNGsC-S308P-H309D-pKK223

    Plasmid
    #131378
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.
    Depositor
    Insert
    EcNGsC MutY chimera S308P H309D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are from…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. EcNGsC-F307A-S308A-pKK223

    Plasmid
    #131379
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.
    Depositor
    Insert
    EcNGsC MutY chimera F307A S308A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are fro…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pJK215

    Plasmid
    #72427
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    Inserts
    succinyl-diaminopimelate desuccinylase
    2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
    acetylglutamate kinase
    ribosome biogenesis GTP-binding protein YsxC
    insertase
    ribonuclease P
    50S ribosomal protein L34
    hypothetical protein
    glyoxylase I
    hypothetical protein
    UDP-N-acetylglucosamine acyltransferase
    3-hydroxyacyl-ACP dehydratase
    UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Tags
    Expression
    Mutation
    missing C-terminal domain residues 277-391(ter) a…
    Promoter
    n/a
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pLentiCMV_HyPer7RgDAAO

    Plasmid
    #217653
    Purpose
    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane
    Depositor
    Insert
    HyPer7 D amino acid oxidase
    Use
    Lentiviral
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Promoter
    CMV
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. pNK3071

    Plasmid
    #219755
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Insert
    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  15. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
  16. pNK497

    Plasmid
    #219754
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Insert
    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 11901 - 11950 of 16985 results