Skip to main content
Addgene
Showing: 921 - 960 of 4777 results

  1. pQE80L-SH3

    Plasmid
    #160331
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.
    Depositor
    Sang Taek Jung
    Article
    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12
    Insert
    SH3
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160331-plasmid-map-sequence-id-326603

    Enlarge

  3. DD-DamV133A-tdTomato-LMNB1

    Plasmid
    #159604
    Purpose
    Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.
    Depositor
    Aaron Streets
    Article
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Insert
    DD-DamV133A-tdTomato-LMNB1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Dam V133A mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159604-plasmid-map-sequence-id-311954

    Enlarge

  5. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Mutation
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

    Enlarge

  6. pCK354

    Plasmid
    #129698
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799
    Depositor
    John Heap
    Article
    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m
    Inserts
    PrhaBAD
    yfp
    rhaS
    Terminator ECK120010799
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129698-plasmid-map-sequence-id-258484

    Enlarge

  9. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

    Plasmid
    #174642
    Purpose
    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)
    Use
    Tags
    SSPB(micro)-Venus
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174642-plasmid-map-sequence-id-346995

    Enlarge

  10. pCK351

    Plasmid
    #129696
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435
    Depositor
    John Heap
    Article
    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m
    Inserts
    PrhaBAD
    yfp
    rhaS
    Terminator ECK120034435
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129696-plasmid-map-sequence-id-258483

    Enlarge

  11. pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)

    Plasmid
    #174639
    Purpose
    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-GFP-ppKinVIb(861-1321)
    Use
    Tags
    SSPB(micro)-GFP
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174639-plasmid-map-sequence-id-346670

    Enlarge

  23. pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)

    Plasmid
    #174641
    Purpose
    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-ppKin14VIb(861-1321)
    Use
    Tags
    SSPB(micro)-Venus
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    174641-plasmid-map-sequence-id-355829

    Enlarge

  25. pCK353

    Plasmid
    #129697
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170
    Depositor
    John Heap
    Article
    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m
    Inserts
    PrhaBAD
    yfp
    rhaS
    Terminator ECK120015170
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129697-plasmid-map-sequence-id-258482

    Enlarge

  26. pCK355

    Plasmid
    #129699
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminator
    Depositor
    John Heap
    Article
    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m
    Inserts
    PrhaBAD
    yfp
    rhaS
    ilvBN terminator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129699-plasmid-map-sequence-id-258506

    Enlarge

  34. pGMβ1

    Plasmid
    #216202
    Purpose
    Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.
    Depositor
    Yasuko Sasaki
    Article
    Iwamoto et al Biosci Microbiota Food Health. 2022;41(1
    Type
    Empty backbone
    Use
    Shuttle vector, conjugal plasmid, theta type rep…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…
    Available since
    Jan. 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    216202-plasmid-map-sequence-id-458484

    Enlarge

Showing: 921 - 960 of 4777 results