We narrowed to 13,546 results for: GAN

1. pBiFC-HA-hCaspase 2 prodomain-VC155

   Plasmid

   #49261

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 prodomain (CASP2 Human)
   

   Tags

   HA and split Venus C

   Expression

   Mammalian

   Mutation

   delete aa 148-435

   Promoter

   CMV

   Available Since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pBIFC-flag-hCaspase 2 prodomain-VN173

   Plasmid

   #49262

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 prodomain (CASP2 Human)
   

   Tags

   flag and split Venus N

   Expression

   Mammalian

   Mutation

   delete aa 148-435

   Promoter

   CMV

   Available Since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pXPR_003 sgSMARCA4 guide 2

   Plasmid

   #193605

   Purpose

   SMARCA4 knockout

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgSMARCA4 guide 2 (SMARCA4 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

   Plasmid

   #115787

   Purpose

   Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   FZD4 (FZD4 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-MBP-TEV-PWWP_DNMT3A

   Plasmid

   #186970

   Purpose

   Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   DNMT3A PWWP domain (DNMT3A Synthetic, Human)
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   PWWP domain (residues 278–427)

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)

   Plasmid

   #156162

   Purpose

   Expresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   THBS1 with deletion of amino acid residues 383-548

   Promoter

   CMV

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TVBB N-term-mNeongreen

   Plasmid

   #169226

   Purpose

   Targeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG416GAL10-HRV-B14_3C

   Plasmid

   #203466

   Purpose

   Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   HRV-B14 3C protease (HRVBgp1 )
   

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. STARR-seq luciferase INF enhancer vector_ORI_OAS3

   Plasmid

   #99324

   Purpose

   Luciferase validation vector with OAS3 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr12: 94575894 -94578286
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Expression

   Mammalian

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBiFC-HA-hCaspase 2 CARD-VC155

    Plasmid

    #49259

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    Caspase-2 CARD (CASP2 Human)
    

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    deleted aa 123-435

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pGV3319

    Plasmid

    #170277

    Purpose

    Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    Bst-LF D720A
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    D720A

    Promoter

    T7-lac

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. QSOX1-bio-His

    Plasmid

    #51825

    Purpose

    Expresses full-length Sulfhydryl oxidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    QSOX1 (QSOX1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV_PP1-mCitrine

    Plasmid

    #172472

    Purpose

    Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
    

    Use

    Lentiviral

    Tags

    mCitrine and prolactin signal sequence (cleaved p…

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

    Plasmid

    #49258

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Tags

    flag and split Venus N

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCINeo-mCHMP5

    Plasmid

    #11771

    Depositor

    Sankar Ghosh

    Article

    Shim et al J Cell Biol. 2006 Mar 27. 172(7):1045-56

    Insert

    chromatin modifying protein 5 (Chmp5 Mouse)
    

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pBiFC-flag-hCaspase 2 CARD-VN173

    Plasmid

    #49260

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 CARD (CASP2 Human)
    

    Tags

    Flag and split Venus N

    Expression

    Mammalian

    Mutation

    delete aa 123-435

    Promoter

    CMV

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pTD80

    Plasmid

    #172901

    Purpose

    Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pY036_ATP1A1_G3_Array

    Plasmid

    #86619

    Purpose

    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEMS2281

    Plasmid

    #111902

    Purpose

    ssAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple346 (NEFM Human)
    

    Use

    AAV

    Promoter

    Ple346 (NEFM MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. miniSOG-LaminB1-10

    Plasmid

    #57771

    Purpose

    Localization: Nuclear Envelope, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pCDH-EF1a-hLMX1A-T2A-copGFP

    Plasmid

    #170444

    Purpose

    Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human LMX1A
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CD226-bio-His

    Plasmid

    #51646

    Purpose

    Expresses full-length CD226 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD226 (CD226 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAG416GAL-EMCV_3C

    Plasmid

    #201941

    Purpose

    Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    EMCV 3C protease (EMCVgp1 )
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBABE-puro-Halo-hMRE11(wt)

    Plasmid

    #208393

    Purpose

    To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Expression

    Mammalian

    Mutation

    H129N

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBABE-puro-Halo-hMRE11(H129N)

    Plasmid

    #208394

    Purpose

    To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Expression

    Mammalian

    Mutation

    H129N

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHH0103_THRA_Hormone-recep

    Plasmid

    #109810

    Purpose

    Protein expression and purification of THRA_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    THRA_Hormone-recep (THRA Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-DCX(S306D)-2A-mCherry

    Plasmid

    #118292

    Purpose

    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    Doublecortin (DCX Human)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    Serine 306 changed to Aspartic Acid

    Promoter

    CMV with beta global intron

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-DCX(S306A)-2A-mCherry

    Plasmid

    #118291

    Purpose

    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanine

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    Doublecortin (DCX Human)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    changed Serine 306 to Alanine

    Promoter

    CMV with beta global intron

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV superYFP-AURKA-mTurq2

    Plasmid

    #157772

    Purpose

    Expression of AuroraA kinase biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and superYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Human b4 nicotinic receptor subunit EM construct

    Plasmid

    #163961

    Purpose

    pEZT-BM vector with beta4 gene after CMV promoter, published EM construct

    Depositor

    Ryan Hibbs

    Article

    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

    Insert

    Human beta4 nicotinic acetylcholine receptor subunit EM construct
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    StrepII tag and apocytochrome b(562)RIL (BRIL)

    Expression

    Mammalian

    Mutation

    Pro341-Ser395 replaced with apocytochrome b(562)R…

    Promoter

    CMV

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ECE1-His-bio

    Plasmid

    #53361

    Purpose

    Expresses full-length Endothelin converting enzyme 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ECE1 (ECE1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

    Plasmid

    #91881

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

33. pRS423-mitoT

    Plasmid

    #174525

    Purpose

    Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    Yeast mitochondrial intermembrane tether
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mouse a5 full length

    Plasmid

    #217810

    Purpose

    Full length mouse integrin a5 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGA5 (Itga5 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid

    #185289

    Purpose

    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    iLID::eGFP::FTH1
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCS2-mKate-MKLP1

    Plasmid

    #140570

    Purpose

    Expresses mKate tagged MKLP1

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    MKLP1 (KIF23 Human)
    

    Tags

    mKate

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pETM33_Orf9b

    Plasmid

    #156485

    Purpose

    Bacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Orf9b
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pX330-NLC-DHFR-SpCas9

    Plasmid

    #124523

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NLC-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)

    Plasmid

    #156163

    Purpose

    Expresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Truncated THBS1 (amino acid residues 1-954)

    Promoter

    CMV

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG426GAL-KUNV_NS2B-GS-NS3

    Plasmid

    #203506

    Purpose

    Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    KUNV NS2B-GS-NS3
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge