We narrowed to 43,521 results for: cha;

1. pCIneo-lambdaN-HA-HsDCP2_1-395_V

   Plasmid

   #147791

   Purpose

   Mammalian Expression of HsDCP2_1-395

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP2_1-395 (DCP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCIneo-lambdaN-HA-HsDCP2-D15AR19A_V

   Plasmid

   #147792

   Purpose

   Mammalian Expression of HsDCP2-D15AR19A

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP2-D15AR19A (DCP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIneo-lambdaN-HA-HsDCP2-F404AF407AF409A_V

   Plasmid

   #147793

   Purpose

   Mammalian Expression of HsDCP2-F404AF407AF409A

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP2-F404AF407AF409A (DCP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIneo-lambdaN-HA-HsEDC4_974-1401_S

   Plasmid

   #147529

   Purpose

   Mammalian Expression of HsEDC4_974-1401

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsEDC4_974-1401 (EDC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. DsRed -7e

   Plasmid

   #191765

   Purpose

   Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Use

   Tags

   N terminal fusion of negatively charged polypetod…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Syn_CapChR1_mScarlet

   Plasmid

   #188037

   Purpose

   Expresses CapChR1-mScarlet in mammalian neurons

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR1-mScarlet
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   S63D, L132C, T159C and N258E mutations in CrChR2

   Promoter

   hSyn

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7-EGFP-C1-HsDCP2-G96PV97P_V

   Plasmid

   #147814

   Purpose

   Mammalian Expression of HsDCP2-G96PV97P

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP2-G96PV97P (DCP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7-EGFP-C1-HsDCP2-K130A_V

   Plasmid

   #147815

   Purpose

   Mammalian Expression of HsDCP2-K130A

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP2-K130A (DCP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7-EGFP-C1-HsDCP2-W44A_V

   Plasmid

   #147816

   Purpose

   Mammalian Expression of HsDCP2-W44A

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP2-W44A (DCP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-EGFP-C1-HsDCP2_1-244_V

    Plasmid

    #147807

    Purpose

    Mammalian Expression of HsDCP2_1-244

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

    Insert

    HsDCP2_1-244 (DCP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7-EGFP-C1-HsDCP2_97-392_V

    Plasmid

    #147809

    Purpose

    Mammalian Expression of HsDCP2_97-392

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

    Insert

    HsDCP2_97-392 (DCP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA 3.1 BS (-) N protein

    Plasmid

    #188448

    Purpose

    SARS CoV-2 N protein expression

    Depositor

    Ajit Chande

    Article

    Mishra et al Life Sci Alliance. 2022 Mar 16;5(7). pii

    Insert

    SARS-CoV-2 N protein
    

    Use

    Tags

    Strep-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJ414-PCY1:S495A

    Plasmid

    #98151

    Purpose

    Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:S495A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed serine 495 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJ414-PCY1:W603A

    Plasmid

    #98153

    Purpose

    Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:W603A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tryptophan 603 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJ414-PCY1:H695Q

    Plasmid

    #98154

    Purpose

    Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:H695Q
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed histidine 695 to glutamine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJ414-PCY1:H695A

    Plasmid

    #98155

    Purpose

    Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:H695A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed histidine 695 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ414-PCY1:Y696G

    Plasmid

    #98156

    Purpose

    Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:Y696G
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tyrosine 696 to glycine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-RanGAP*

    Plasmid

    #175237

    Purpose

    Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-FKBP-E2

    Plasmid

    #175243

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FKBP-EGFP-RanGAP

    Plasmid

    #175236

    Purpose

    Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-anticalin-FLN-ELP-His-ybbr

    Plasmid

    #168039

    Purpose

    Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-SdrG-FLN-ELP-His-ybbr

    Plasmid

    #168047

    Purpose

    Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    

    Use

    Tags

    3xELP linker, 6x Histag, ddFLN4, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-GFAP104-ChromeT-mCherry

    Plasmid

    #123126

    Purpose

    AAV-mediated expression of ChromeT-mCherry under the GFAP promoter.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeT-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

    Promoter

    GFAP104

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. THI ONLY pALD4 T-

    Plasmid

    #161497

    Purpose

    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7gRNA-smyhc1_977

    Plasmid

    #140867

    Purpose

    sgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1).

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    zebrafish smyhc1_977 sgRNA for in vitro transcription (smyhc1 Zebrafish, Synthetic)
    

    Use

    In vitro transcription of sgrnas

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LIC-Z-Y-L

    Plasmid

    #153546

    Purpose

    this is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    with 6 Y in 3 ITAMs mutated to L

    Promoter

    CMV

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SNX17-PX (1-111)

    Plasmid

    #119098

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX17-PX (1-111)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX17-PX (1-111) (SNX17 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SNX16-PX (104-216)

    Plasmid

    #119097

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX16-PX (104-216)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX16-PX (104-216) (SNX16 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SNX4-PX (48-186)

    Plasmid

    #119085

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX4-PX (48-186)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX4-PX (48-186) (SNX4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SNX32-PX (17-166)

    Plasmid

    #119111

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX32-PX (17-166)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX32-PX (17-166) (SNX32 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SNX7-PX (89-218)

    Plasmid

    #119088

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX7-PX (89-218)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX7-PX (89-218) (SNX7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. DsRed2-M11-92

    Plasmid

    #134467

    Purpose

    M11-92 expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin Amino Acids 1-92 (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M1 Spastin Amino Acids 1-92

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mEmerald-M11-92

    Plasmid

    #134465

    Purpose

    M11-92 expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin Amino Acids 1-92 (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M1 Spastin Amino Acids 1-92

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKdH_P-GAP-ScADH2

    Plasmid

    #126734

    Purpose

    yeast plasmid overexpressing ADH2 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKdH_P-GAP-ScADH2+P-GAP*-ScALD6

    Plasmid

    #126737

    Purpose

    yeast plasmid overexpressing ADH2 and ALD6 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2,ald6
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. FLAG-TET1-746-951(pL72)

    Plasmid

    #124406

    Purpose

    TET1-746-951, containing SID, used for Co-IP.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    FLAG-TET1-746-951 (TET1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJET1.2_Nkx3-2_Anti-Sense

    Plasmid

    #124438

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    NK3 homeobox 2 (Nkx3-2 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-Syn-ChromeT-GFP

    Plasmid

    #123316

    Purpose

    AAV-mediated expression of ChromeT-GFP under the Syn promoter.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

    Promoter

    Syn

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PD-L1-miSFIT-9-G, 15-C

    Plasmid

    #124688

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJET1.2_Nkx3-2_Sense

    Plasmid

    #124437

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    NK3 homeobox 2 (Nkx3-2 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge