We narrowed to 16,480 results for: por

1. pEN_TT 3xFLAG-KPNA2

   Plasmid

   #192306

   Purpose

   Gateway entry vector for an inducible 3xFLAG-KPNA2

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   KPNA2 (KPNA2 Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-Ef1a-SunTag-RPS7-PP7-WPRE

   Plasmid

   #198338

   Purpose

   PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   GCN4x24-RPS7-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops and SunTag

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO-VGAT-WPRE-pA

   Plasmid

   #39320

   Depositor

   Bernardo Sabatini

   Article

   Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

   Insert

   Slc32a1 (Slc32a1 Mouse)
   

   Use

   AAV; R

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500

   Plasmid

   #92228

   Purpose

   Expression of zmADH using the p70a promoter and UTR1

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   NTerm 6xHis Tagged zmADH
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1K245 GLUT1-GFP

   Plasmid

   #200097

   Purpose

   human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   GLUT1 (SLC2A1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K-->R mutations of amino acids 6, 7, 225, 229,…

   Promoter

   CMV

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBa.GFP-KIF13B tail 442-1843

   Plasmid

   #134623

   Purpose

   Mammalian expression of mouse KIF13B 442-1843

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF13B (Kif13b Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 441 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PDZb-beta2(616-951)-mCherry

   Plasmid

   #100736

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   PDZb and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCK314

   Plasmid

   #110545

   Purpose

   Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

   Inserts

   Modified PrhaBAD (CRP-binding sites removed)
   yfp
   rhaS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. FastLightR-bRaf-mVenus

   Plasmid

   #162155

   Purpose

   Expresses FastLightR-bRaf-mVenus in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   FastLightR-bRaf-mVenus
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Valine 600 is substituted with Glutamic acid

   Promoter

   CMV

   Available since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNS19-LV-mutRFP-2A-GFP

    Plasmid

    #113716

    Purpose

    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    mutRFP-2A-GFP-IRES-Puro
    

    Use

    Lentiviral; Reporter system

    Tags

    Expression

    Mammalian

    Mutation

    Y64L (mutRFP)

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPonA-BI-Gl NORM-LacZA TER- LacZB

    Plasmid

    #86212

    Purpose

    beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter

    Depositor

    Robert Singer

    Article

    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

    Insert

    beta-Globin (HBB, Hbb Mouse, Human)
    

    Use

    Ponasterone a inducible promoter

    Tags

    lacZA or lacZB

    Expression

    Mammalian

    Mutation

    39TER in beta Globin in MCS II

    Promoter

    Ponasterone A inducible

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. shFGF2#2

    Plasmid

    #157668

    Purpose

    Tet inducible knockdown of FGF2

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-sgRNA_Ago2_1

    Plasmid

    #73529

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pX330-sgRNA_Ago2_2

    Plasmid

    #73530

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid

    #84636

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub(TA)-YPet-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9-Puro HLAG-2A-sgRNA

    Plasmid

    #182551

    Purpose

    Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

    Depositor

    Santiago Miriuka

    Article

    Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

    Insert

    hSpCas9-2A-Puro-HLAG-2A-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. prk5-MFSD12-TEV-3xFLAG-3xHA

    Plasmid

    #149504

    Purpose

    Transient expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Transient expression

    Tags

    TEV-3XHA-3XFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized Sequence

    Promoter

    CMV

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-soCoChR-GFP

    Plasmid

    #107710

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

    Plasmid

    #188541

    Purpose

    Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

    Depositor

    Darrell Kotton

    Article

    Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mcherry Gamma 9 ---pcDNA3.1

    Plasmid

    #64203

    Purpose

    G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    G protein Gamma 9 (GNGT2 Human)
    

    Use

    Tags

    6xHis and mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

    Plasmid

    #90014

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-TNRC6C
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPN062

    Plasmid

    #91595

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPN063

    Plasmid

    #91596

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ALARM NMR

    Plasmid

    #90209

    Purpose

    Expresses the human La antigen for use in ALARM NMR

    Depositor

    Michael Walters

    Article

    Dahlin et al Nat Commun. 2017 Nov 15;8(1):1527. doi:

    Insert

    La antigen (SSB Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    contains amino acids 100-324

    Promoter

    T7 promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FLAG-SFPQY527A

    Plasmid

    #166961

    Purpose

    Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ-Y527A (SFPQ Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    SFPQ-Y527A

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #196545

    Purpose

    Donor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    DelRosso et al Nature. 2023 Apr;616(7956):365-372. doi:

    Insert

    Surface marker and Citrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-pmEMBer

    Plasmid

    #174441

    Purpose

    Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEMBer
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET24a-VHH-tev-std

    Plasmid

    #109423

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET-32a(+)-TGFB3m

    Plasmid

    #120289

    Purpose

    Produce human recombinant mTGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    monomeric Transforming Growth Factor-β3
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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