We narrowed to 16,751 results for: IGH@

1. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

2. AAV-Camk2-NES-Twitch-NR

   Plasmid

   #173043

   Purpose

   TnC-based calcium sensor with green-red FRET pair ratiometric readout

   Depositor

   Yiyang Gong

   Article

   Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

   Insert

   NES-Twitch-NR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamK2

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDB179-DepoPETase

   Plasmid

   #218702

   Purpose

   Encodes codon optimized DepoPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   DepoPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCS-mClover3

   Plasmid

   #74236

   Purpose

   Constitutive expression of mClover3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Has Service

   Cloning Grade DNA

   Insert

   mClover3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIVT-iCre

   Plasmid

   #232773

   Purpose

   IVT template for making modRNA encoding iCre

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   iCre
   

   Use

   Cre/Lox; In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. mNeonGreen-DEVD-NanoLuc

   Plasmid

   #98287

   Purpose

   caspase-3 BRET Sensor

   Depositor

   Maarten Merkx

   Article

   den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

   Insert

   mNeonGreen-LEHD-NanoLuc
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pComb3XLambda

   Plasmid

   #63892

   Purpose

   Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Insert

   human antibody lambda light chain (IGLL5 Human)
   

   Use

   Phage display

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBECAb-apr

   Plasmid

   #122001

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EC64422

   Plasmid

   #191768

   Purpose

   CRISPR mutagenesis in Medicago truncatula

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-Cre.zf1

    Plasmid

    #61391

    Purpose

    mRNA synthesis of Cre recombinase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Cre.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEBB HA cIAP1

    Plasmid

    #38232

    Depositor

    Colin Duckett

    Article

    Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCMV Puro DEST ERKKTRClover

    Plasmid

    #59150

    Purpose

    Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Bxb1-GA_CAG-mStayGold

    Plasmid

    #229802

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

    Plasmid

    #209727

    Purpose

    Expresses cytosolic medium affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95K.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKIR1.1

    Plasmid

    #85758

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5A

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDB179-DuraPETase

    Plasmid

    #218699

    Purpose

    Encodes codon optimized DuraPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    DuraPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AB65_pCAG.Cas9-D10A.rBGpA

    Plasmid

    #199256

    Purpose

    Expression construct encoding the SpCas9-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SpCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-FLAG-Ku80

    Plasmid

    #46958

    Purpose

    Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    Ku80 (XRCC5 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. AsCas12a-6xNLS-E174R/S542R (pRG232)

    Plasmid

    #149723

    Purpose

    Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

    Depositor

    Junwei Shi

    Article

    Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p2CT-His-MBP-Lbu_C2c2_WT

    Plasmid

    #83482

    Purpose

    Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTlpA_mCherry_Txe/Axe

    Plasmid

    #229147

    Purpose

    Toxin-Antitoxin (Txe/Axe)

    Depositor

    Shrikrishnan Sankaran

    Article

    Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

    Insert

    mCherry, Txe/Axe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtlpA (mCherry), Txe/Axe (Native)

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTrcHIS-7D12Gln13TAG

    Plasmid

    #125264

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    Gln13TAG 7D12
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPAmCherry-b-actin

    Plasmid

    #31949

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    b-actin

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRPF215

    Plasmid

    #106377

    Purpose

    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

    Depositor

    Robert Fagan, Neil Fairweather

    Article

    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

    Insert

    Ptet-himar1-Ter(slpA)-transposon
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Codon optimised for C. difficile

    Promoter

    Ptet

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-REPORT (PGK) NanoLuc

    Plasmid

    #208576

    Purpose

    Empty NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    NanoLuciferase
    d2eGFP
    Empty response element
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and miniP

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pmScarlet-i_C1

    Plasmid

    #85044

    Purpose

    Mammalian expression of mScarlet-i

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet-i
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pORE303N

    Plasmid

    #194438

    Purpose

    CRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI site for gRNA insertion

    Depositor

    Chung-Jui Tsai

    Article

    Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCH49 (crRNA expression vector)

    Plasmid

    #217338

    Purpose

    CROP-seq crRNA expression vector with GFP and neo marker

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    AsDR-filler-AsDR8, GFP-neo
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-His-Cre

    Plasmid

    #123128

    Purpose

    Bacterial expression plasmid for wild-type Cre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEmpty_Control

    Plasmid

    #162607

    Purpose

    Empty control for Cas9 experiments and to clone new guide RNAs for paper

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge