We narrowed to 7,692 results for: Lif;
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Plasmid#177178PurposePEAR-GFP target plasmid with split EGFP and disrupted 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMPP-mCherry-hTRIM21
Plasmid#104972PurposeLentiviral Transfer Plasmid with mCherry-tagged human TRIM21DepositorAvailable SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-LEU2
Plasmid#157656PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequenceDepositorInsertGFP-gRNA
UseInsert storage (replicative in e. coli)TagsGFP-gRNAAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
TagsC terminal TEV GFP octa-His affinity tagExpressionBacterialPromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-FAK W266A
Plasmid#186149PurposeFor expressing FAK in mammalian cellsDepositorAvailable SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDAS12124_PEAR-GFP-preedited
Plasmid#177179PurposePEAR-GFP control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
Tags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SB_concave
Plasmid#110102PurposepBXPHM3 with scaffold Sybody of the concave libraryDepositorInsertscaffold Sybody of the concave library
TagsHisExpressionBacterialPromoterPBADAvailable SinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-CaNAR2
Plasmid#64729PurposeCytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertCaNAR2-NES
TagsCerulean3, Nuclear export signal (NES), and YPetExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
eMagB-iRFP-Mito
Plasmid#162250PurposeBlue-light dependent heterodimerization systemDepositorInserteMagB-iRFP-OMP25
ExpressionMammalianPromoterbeta-actin promoterAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDAS12395_PEAR-GFP_2.0-preedited
Plasmid#177185PurposePEAR-GFP3 control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GFP
Plasmid#86537PurposeRetrovirus expressing GFPDepositorInsertGFP
UseRetroviralExpressionMammalianPromoterSynapsin I (human)Available SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
LTR5HS S. aureus scaffold CARGO array
Plasmid#191317PurposeContains an array of guide RNAs targeting LTR5Hs elementsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cys-3HB-SPYcatcher
Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-KRAB-2A-Puro
Plasmid#129536PurposePiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.DepositorInsertPB-PGK-tetR-KRAB-2A-Puro
UseUnspecifiedPromoterhPGK promoterAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2
Plasmid#87644PurposeFor creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2DepositorInsertmyr::SNAPf>myr::CLIPf>myr::Halo2
ExpressionInsectAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-GFP-2A-SynmRuby
Plasmid#166608PurposeEncodes Cre-dependent GFP-2A-synaptophysinmRuby under control of the TREDepositorInsertGFP-2A-syn
UseAAVPromotertetracycline response elementAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS9
Plasmid#111762PurposeExpresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS9-mRuby2 fusion
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable SinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT003-GPM
Plasmid#163383PurposeTo express sGFP module in the G-baToN systemDepositorInsertGFP-PDGFRtm-2A-mCherry (GPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianPromoterEF1aAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS5
Plasmid#111760PurposeExpresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS5-mRuby2 fusion
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable SinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_R261A
Plasmid#202586PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Arginine 261 to AlanineAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ER-mCherry-eMagA
Plasmid#162248PurposeBlue-light dependent heterodimerization systemDepositorInsertCytP450 ER targeting sequencel-mCherry-eMagA
ExpressionMammalianPromoterbeta-actin promoterAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-mito
Plasmid#113919PurposePlasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
ExpressionMammalianPromoterCMV TetO2Available SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV9cap
Plasmid#198016PurposeFor high-level expression of the AAV9 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV9 cap
UseAAVExpressionMammalianMutationWTPromoterCMVAvailable SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-5-SV40 polyA
Plasmid#190869PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode5
UseAAVMutationWTAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP
Plasmid#119903PurposeBxb1 integration plasmid for cuO array with Puro selection cassetteDepositorInsertcuOx144 array
UseMouse TargetingAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pEBTet_NADP-Snifit-cyto
Plasmid#113917PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
ExpressionMammalianPromoterCMV TetO2Available SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCMV_PACLight1
Plasmid#197864PurposeMammalian expression of PACLight1 biosensor for PACAP (Pituitary Adenylate Cyclase Activating Peptide)DepositorInsertPACLight1
ExpressionMammalianPromoterCMVAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Txnip(C247S)
Plasmid#206353PurposeAAV plasmid expressing mutant TXNIPDepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-jGCaMP7b-XC
Plasmid#182221PurposeImproved genetic encoded calcium sensor based on GCaMP7b.DepositorInsertjGCaMP7b-XC
UseAAVExpressionMammalianAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT001-LVB
Plasmid#163381PurposeSecond generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN systemDepositorInsertLaG17-VEEGFRtm-BFP (LVB)
UseLentiviralTagsBFPExpressionMammalianPromoterEF1AAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-hM3Dq-mCherry
Plasmid#166599PurposeEncodes Cre-dependent hM3Dq-mCherry under control of the TREDepositorInserthM3Dq-mCherry
UseAAVPromotertetracycline response elementAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-GCaMP3
Plasmid#64853PurposeCytosol-targeted version of the Ca2+ indicator GCaMP3.DepositorInsertGCaMP3
TagsNuclear export signal (NES)ExpressionMammalianPromoterCMVAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cyto-CaNARi
Plasmid#64849PurposeCytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.DepositorInsertCaNARi
TagsCerulean, Nuclear export signal (NES), and VenusExpressionMammalianPromoterCMVAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant mNG-ORP8
Plasmid#195170Purposemammalian expression of siRNA-resistant ORP8 tagged with mNeonGreenDepositorInsertORP8 (OSBPL8 Human)
TagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from K174 t…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-ChR2-TdTomato
Plasmid#166600PurposeEncodes Cre-dependent ChR2-TdTomato under control of the TREDepositorInsertChR2-TdTomato
UseAAVMutationH134RPromotertetracycline response elementAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-DNAPKcs HRD
Plasmid#207088PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBMN-dGBP1-TagBFP
Plasmid#80087PurposeMMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection markerDepositorInsertdGBP1-TagBFP
UseRetroviralExpressionMammalianAvailable SinceFeb. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry- eMagBF - 5ptaseOCRL
Plasmid#162254PurposeBlue-light dependent heterodimerization systemDepositorInsertmCherry- eMagBF - 5ptaseOCRL
ExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FIP3
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialPromoterT7Available SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cys-30nmSAH-SPYcatcher
Plasmid#214839PurposeThis plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-30nmSAH-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-Control
Plasmid#122277PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseExpressionBacterialPromoterThymidine kinaseAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-SADB19_oG-IRES-Puro
Plasmid#172367PurposeGeneration of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic PuromycinDepositorInsertoG + Puro
UseRetroviralExpressionMammalianPromoterCAGAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
P427-FBP_13
Plasmid#139704PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI
Plasmid#205162Purposeexpress scFv-GCN4-HAtag-msfGFP(V206K)-GBIDepositorInsertscFv-GCN4-HAtag-msfGFP(V206K)-GBI
ExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lys-eMagB-iRFP
Plasmid#162249PurposeBlue-light dependent heterodimerization systemDepositorInsertp18-eMagB-iRFP
ExpressionMammalianPromoterbeta-actin promoterAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-BFLF1
Plasmid#162657PurposeExpresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertBFLF1
TagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only