164,826 results

1. pLenti-EFS-MCS-WPRE

   Plasmid

   #163362

   Purpose

   The empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectors

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCMV_DENV4_PrME

   Plasmid

   #207266

   Purpose

   Mammalian expression of Dengue virus 4 PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   DENV4_PrME (POLY Dengue virus 4)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac-CHD7

   Plasmid

   #170141

   Purpose

   Expresses CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ApaLI*-HA

   Plasmid

   #211461

   Purpose

   Express mitochondrial localized ApaLI with D211A, E225A and K227A mutations

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI mutant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D211A, E225A, K227A

   Promoter

   CMV

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDestTol2pA2-U6:gRNA

   Plasmid

   #63157

   Purpose

   Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 Flag hHMGB1

   Plasmid

   #31609

   Depositor

   Yasuhiko Kawakami

   Article

   Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

   Insert

   HMGB1 (HMGB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. mRNA-CleanCap entry vector

   Plasmid

   #188249

   Purpose

   Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Green cGull

   Plasmid

   #86867

   Purpose

   Green fluorescent probe for cGMP

   Depositor

   Tetsuya Kitaguchi

   Article

   Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

   Insert

   Green cGull
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB551

   Plasmid

   #74881

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid

    #154786

    Purpose

    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

    Depositor

    Robert Singer

    Article

    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

    Insert

    Adenosine deaminse (Adar Synthetic, Fly)
    

    Use

    Lentiviral

    Tags

    GFP, V5, and mCherry

    Expression

    Mammalian

    Mutation

    Synonymized serine to prevent autoediting of ADAR…

    Promoter

    UBC

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.2-3xFLAG-V5-ccdB

    Plasmid

    #87072

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-iLACCO1.2

    Plasmid

    #218823

    Purpose

    Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-HA-Ubiquitin-K29

    Plasmid

    #22903

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K29 only, all other lysines mutated to arginines

    Promoter

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCBI-CRY2PHR-VPR

    Plasmid

    #192606

    Purpose

    Plasmid for constitutive expression of CRY2PHR-VPR.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    CRY2PHR-VPR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28sumo-hRig-I-Full-length

    Plasmid

    #210827

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Structure. 2012 Nov 7;20(11):1983-8. doi

    Insert

    hRIG-I (DDX58) Full length protein (RIGI Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInt-ts

    Plasmid

    #66076

    Purpose

    The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    lambda integrase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-BAF

    Plasmid

    #101772

    Purpose

    Expresses EGFP tagged BAF in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. hKDR

    Plasmid

    #83436

    Purpose

    human VEGFR2 with V5 and tGFP tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

    Insert

    human VEGFR2 (KDR Human)
    

    Use

    Tags

    V5 and turboGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL1777 (pEffector)

    Plasmid

    #160731

    Purpose

    Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins and crRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBT2-4xUAS:NICD-P2A-mRFP

    Plasmid

    #127594

    Purpose

    Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

    Depositor

    Longhou Fang

    Article

    Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

    Insert

    NICD (notch1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGP-AAV-syn-iGABASnFR2n-WPRE

    Plasmid

    #218875

    Purpose

    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    Synapsin

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. ANO6-Plvx-mCherry-c1

    Plasmid

    #62554

    Purpose

    Full length mouse ANO6 tagged with mCherry on the C-terminus

    Depositor

    Renzhi Han

    Article

    Zhao et al PLoS One. 2014 Mar 24;9(3):e92749. doi:

    Insert

    mouse ANO6 (Ano6 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMSCV PIG (Puro IRES GFP empty vector)

    Plasmid

    #21654

    Purpose

    Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLSB-HYG

    Plasmid

    #166699

    Purpose

    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    adh15 promoter and tRNA promoter

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pICH41308

    Plasmid

    #47998

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pOpen-EcoRV

    Plasmid

    #165548

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    EcoRV
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. Halo-VSVG-RUSH

    Plasmid

    #166907

    Purpose

    RUSH cargo: Str-Ii_IRES_VSVG-SBP-Halo

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    HaloTag
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIG-828_HA-GD2-28z_CAR_BATF_Retroviral

    Plasmid

    #207505

    Purpose

    This plasmid can be used to generate retrovirus.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    BATF, HA-GD2-28z_CAR (BATF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGGC011

    Plasmid

    #48825

    Purpose

    Provides alcR as GreenGate coding sequence module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    alcR
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-mDlx-GFP-Fishell-1

    Plasmid

    #83900

    Purpose

    GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    mDlx

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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