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Plasmid#170513PurposegRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6:3Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only
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Plasmid L5 attB:Psmyc double blue
Plasmid#170050PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blueDepositorInsertsmTagBFP2
CO BFP
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAR357_SFP_pCDF-1b
Plasmid#122851PurposeCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.DepositorInsertSfp
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green
Plasmid#170048PurposeTri-Responsive Reporter Gene Construct for redgreen, triple red, and double greenDepositorInsertsmScarlet
COmScarlet
mRuby3
mWasabi dN
mWasabi CO
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-mCherry
Plasmid#114013PurposeEmpty sgRNA expression vector with co-expression of mCherry reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-GFP
Plasmid#114012PurposeEmpty sgRNA expression vector with co-expression of TurboGFP reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE1109
Plasmid#202773PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB:Psmyc double green
Plasmid#169412PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double greenDepositorInsertsmWasabi
mWasabi CO
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pNeuroD-ires-GFP
Plasmid#61403PurposeMammalian expression vector under the neuron-specific promoter NeuroD. Co-expression of GFP with IRES sequenceDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNeuroDAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLIK DN RhoA
Plasmid#84646PurposeLentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of VenusDepositorInsertRhoA (RHOA Human)
UseLentiviralTagsMycExpressionMammalianMutationDominant NegativePromoterAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW
Plasmid#133359PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
FU - dio YFP - W
Plasmid#73858PurposeFor expressing YFP in mammalian cells upon Cre co-expression. Can be made into Lentivirus.DepositorInserteYFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Roxed-Cre
Plasmid#51273PurposeComponent of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by DreDepositorInsertRoxed-Cre
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL2-mGold-P2A-EBFP2
Plasmid#157998PurposeCo-expression of mGold and EBFP2 in mammalian cells.DepositorInsertmGold-P2A-EBFP2
UseTagsExpressionMammalianMutationmGold is mVenus with L46F;T63S mutationsPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf
Plasmid#175524PurposeCo-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cellsDepositorInsertBeta4Gal-HaloTag7 and Lyn11-SNAPf
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-iRFP670-Tdtomato-GFP
Plasmid#111817PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsiRFP670
Tdtomato
GFP
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-shTACC3
Plasmid#59355PurposeKnocks down TACC3 in mammalian cells via shRNA and co-expresses GFP.DepositorInsertEGFP
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170809PurposeLentiviral vector to co-express an spsgRNA with NLS-mNeonGreenDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-GFP-Tdtomato-iRFP670
Plasmid#111812PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
Tdtomato
iRFP670
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMVP-Cas9FL-P2A-turboGFP
Plasmid#80941PurposePlasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.DepositorInsertCas9FL
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-NLS-HA-Dre-P2A-BFP
Plasmid#51277PurposeCo-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoterDepositorInsertNLS-HA-Dre-P2A-BFP
UseTagsExpressionMutationPromoterhGFAPAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPH1
Plasmid#177662PurposeRecombinant protein synthesis of human HSPH1DepositorInsertHSPH1 (HSPH1 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-ZHER-mgSrtA
Plasmid#125793Purposefor co-expression of ZHER (a HER2 binding affibody) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of HER2 positive cells.DepositorInsertZHER-mgSrtA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VinculinI997A_TurboID_Venus
Plasmid#200274PurposeExpressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculinI997A_TurboID_Venus
UseTagsExpressionMammalianMutationchange isoleucine to alanine at 997 of vinculinPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH2B-SCFP1-2A-mTq2-NES
Plasmid#60491PurposeCo-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasmDepositorInsertsH2B-SCFP1
mTurquoise2-NES
UseTagsSCFP1 and mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES
Plasmid#60492PurposeCo-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasmDepositorInserts3xnls-SGFP2(T65G)
SGFP2-3xNES
UseTagsSGFP2 and SGFP2(T65G)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMVP-Cas9C-P2A-turboGFP
Plasmid#80935PurposePlasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.DepositorInsertCas9C
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-PT2A-GFP-iRFP670-Tdtomato
Plasmid#111813PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
iRFP670
Tdtomato
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-PT2A-iRFP670-GFP-Tdtomato
Plasmid#111816PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsiRFP670
GFP
Tdtomato
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSA120
Plasmid#59789PurposeC. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operonDepositorInsertshsp-16.41 promoter
zif-1 coding
gpd-2/gpd-3 intergenic sequence
mCherry (worm)
unc-54 3' UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-Tdtomato-iRFP670-GFP
Plasmid#111815PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsTdtomato
iRFP670
GFP
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_NEMF
Plasmid#127126DepositorInsertgRNA NEMF (NEMF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH95pUp95GW (B4)
Plasmid#74013Purposelentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNADepositorInsertPsd95 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
LPA2R-P2A-mCherry
Plasmid#112932PurposePlasmid co-producing the LPA2 receptor and a mCherryDepositorInsertLPA2 receptor
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLXIN-Ap1g1-FKBP
Plasmid#46946PurposeRetroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cellsDepositorInsertAp1g1-FKBP
UseRetroviralTagsMainly mouse Ap1g1 cDNA, with a fragment of brain…ExpressionMammalianMutationPromoterAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-QtgRNA2.1-8R
Plasmid#170514PurposegRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6:3Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-hLYZ
Plasmid#83924PurposeCo-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..DepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170811PurposeLentiviral vector to co-express an sasgRNA with NLS-mNeonGreenDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-Tdtomato-GFP-iRFP670
Plasmid#111814PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsTdtomato
GFP
iRFP670
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinI997A_TurboID_Venus
Plasmid#200273PurposeLentivirus vector expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculinI997A_TurboID_Venus
UseLentiviralTagsExpressionMutationPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE1177
Plasmid#202775PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-wtBlc
Plasmid#168258PurposeN-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip proteinDepositorInsertN-fragment of wtBlc + Leucine Zipper
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-wtBlc
Plasmid#168257PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondinglyDepositorInsertC-fragment of wtBlc
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-CMV
Plasmid#133366PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-CMV-Luc
Plasmid#133367PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PIR702W-Luc
Plasmid#133362PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertIRFP-702
UseTagsHA-tagged IRFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PIR702W
Plasmid#133361PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertIRFP-702
UseTagsHA-tagged IRFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-Luc
Plasmid#133360PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only