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179,521 results

Showing: 10761 - 10800 of 179521 results

  1. pcDNA3.1-GGamma9-GFP2

    Plasmid
    #140991
    Purpose
    Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma9-GFP2 (GNGT2 Human)
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140991-plasmid-map-sequence-id-521071

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  2. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140973-plasmid-map-sequence-id-300655

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  5. hCMV-IE1:Renilla

    Plasmid
    #118066
    Purpose
    Transcriptional unit for constitutive expression of Renilla in mammalian cells
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CMV:GreenRenilla:bGHF
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118066-plasmid-map-sequence-id-240497

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  7. HA-Rab7A

    Plasmid
    #131417
    Purpose
    Expresses HA-tagged human Rab7A in mammalian cells
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    human Rab7A
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131417-plasmid-map-sequence-id-258194

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  8. pENTR_L1-YFP-miniTurbo-L2

    Plasmid
    #127352
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, V5, and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127352-plasmid-map-sequence-id-246314

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  10. pLVX TRE 3G BFP

    Plasmid
    #128070
    Purpose
    Lentiviral expression of Tet inducible TagBFP
    Depositor
    Oskar Laur
    Insert
    TagBFP
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    no
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128070-plasmid-map-sequence-id-255190

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  11. pCMV-2E2-HA-mCh

    Plasmid
    #129596
    Purpose
    Encodes anti-HA frankenbody variant fused with mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Publication
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 27, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129596-plasmid-map-sequence-id-255026

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  17. pCFD3.1-w-dU6:3gRNA

    Plasmid
    #123366
    Purpose
    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).
    Depositor
    Simon Bullock
    Insert
    U6:3-gRNA
    Use
    CRISPR
    Expression
    Insect
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123366-plasmid-map-sequence-id-242548

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  24. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  28. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Publication
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112139-plasmid-map-sequence-id-217823

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  33. PtPuc3_diaCas9_sgRNA

    Plasmid
    #109219
    Purpose
    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum
    Depositor
    Per Winge
    Publication
    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR and Synthetic Biology
    Promoter
    LHCF1 terminator, LHCF2 promoter, U6 3' regi…
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109219-plasmid-map-sequence-id-260727

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  36. pYPQ173

    Plasmid
    #99907
    Purpose
    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
    Depositor
    Yiping Qi
    Publication
    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(
    Insert
    pco-dCas9-VP64-T2A-MS2-VP64
    Use
    CRISPR
    Expression
    Plant
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    99907-plasmid-map-sequence-id-210432

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Showing: 10761 - 10800 of 179521 results