170,806 results

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LcV2-Hygro

Plasmid

#91977

Purpose

LentiCRISPR v2 with Hygro cloned in place of Puro

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-CAXX

Plasmid

#184451

Purpose

Plasma membrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pQFT-mNG

Plasmid

#203675

Purpose

pQFT with mNeonGreen under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Insert

mNeonGreen

Expression

Bacterial

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1-alpha-IIb

Plasmid

#27288

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin alpha IIb (ITGA2B Human)

Tags

His and V5

Expression

Mammalian

Mutation

n/a

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pAL120-TK

Plasmid

#22053

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGWB402-omega

Plasmid

#74797

Purpose

Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

2xCaMV35S enhancer and omega

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBR HPV31 9E

Plasmid

#221009

Purpose

Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322

Depositor

Alison McBride

Insert

Human papillomavirus type 31 genome 9E isolate

Use

Cloning vector

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE zeo PPP2CA Wt

Plasmid

#10689

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-2

Plasmid

#13004

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 2 (SULF2 Human)

Tags

6x His and Myc

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pBbA6c-RFP

Plasmid

#35290

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Article

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pGP-pcDNA3.1 Puro-CAG-Positron2

Plasmid

#239079

Purpose

Mammalian expression of positive-going voltage sensor

Depositor

GENIE Project

Article

Eric R. et al Janelia Figshare 2023

Insert

Positron2

Expression

Mammalian

Mutation

R78K N81D D92N W178F

Promoter

CAG

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9-ABE-Umax-rest1

Plasmid

#222144

Purpose

Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP1337

Plasmid

#119270

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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SPYtag-Histag-Avidin

Plasmid

#214836

Purpose

This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-Histag-Avidin

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-mGSDMD

Plasmid

#111560

Purpose

Express mouse GSDMD in E.coli

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (Gsdmd Mouse)

Tags

His, SUMO

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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SFFV-AID-NTERM-GFP

Plasmid

#185763

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, AID

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptac

Plasmid

#218357

Purpose

Empty vector with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2437

Plasmid

#142943

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF280D (ZNF280D Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLG006 (Ec67 retron)

Plasmid

#157884

Purpose

Expresses the Ec67 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec67 retron

Expression

Bacterial

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC2

Plasmid

#183298

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-FOXP3

Plasmid

#153147

Purpose

Expression of Flag tagged human FOXP3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXP3 (FOXP3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-UBF

Plasmid

#17656

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

UBF (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

Plasmid

#101209

Purpose

Bacterial expression plasmid for SpCas9-HF1 variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A/Q926A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A, Q695A and Q926A

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FLAG-CHK1

Plasmid

#86875

Purpose

To express CHK1 with a FLAG tag at N-terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

CHEK1 (CHEK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-mouse Plaat3 (WT)

Plasmid

#167987

Purpose

Expresses EGFP-Plaat3 in mammalian cells

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls3 (Plaat3 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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DL146_plenti_TRE_GFP-iPAK4

Plasmid

#187446

Purpose

Tet-On inducible expression of eGFP-iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box-Pak4

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-Ezh2

Plasmid

#24926

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

June 27, 2010

Availability

Academic Institutions and Nonprofits only

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pLJSRSF7

Plasmid

#64693

Purpose

His-MBP-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Tags

CBD, HisTag, MBP, NpuDnaE-N102, and Smt3

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 H2B-mIFP T2A Mpro (C145A)

Plasmid

#163080

Purpose

Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

Depositor

Xiaokun Shu

Insert

Inactive Mpro mutant C145A and H2B-mIFP

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC19A1

Plasmid

#132266

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC19A1 (SLC19A1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLX311-Cas9

Plasmid

#118018

Purpose

Constitutive expression of Cas9

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Cas9

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc13-SpyTag_Δpvc10

Plasmid

#243740

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SpyTag - deleted spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SpyTag_Δpvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB3-T4gp43(WT)

Plasmid

#228203

Purpose

Expression in BL21(DE3) of WT T4 DNA polymerase

Depositor

Stephen Benkovic

Insert

43

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-CMV-Galpha-13 ONE-GO

Plasmid

#189740

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-13 ONE-GO
GNA13 (GNA13 Human)

Use

Lentiviral

Mutation

L342M (present in YFP but no functional consequen…

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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170,806 results