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179,521 results

Showing: 10801 - 10840 of 179521 results

  4. pCMV-BE-zCas9

    Plasmid
    #101739
    Purpose
    expression of an optimized Cas9 for base-editing in zebrafish
    Depositor
    Shuo Lin
    Publication
    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1-XTEN-zCas9-UGI-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV
    Available since
    Jan. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101739-plasmid-map-sequence-id-198316

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  5. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

    Plasmid
    #100833
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100833-plasmid-map-sequence-id-198271

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  6. pcDNA3-neo-cterminal-3HA

    Plasmid
    #102643
    Purpose
    To generate three copies of the HA epitope tag on cDNAs.
    Depositor
    Michael E. Wright
    Type
    Empty backbone
    Tags
    3x HA (YPYDVPDYA)
    Expression
    Mammalian
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102643-plasmid-map-sequence-id-196911

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  8. AbVec2.0-IGHG3

    Plasmid
    #99577
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99577-plasmid-map-sequence-id-192592

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  11. pMito-TrxRFP1

    Plasmid
    #98997
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized
    Depositor
    Huiwang Ai
    Publication
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TrxRFP1 and mitochondrial localization sequence
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98997-plasmid-map-sequence-id-189692

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  19. pSG5-HA-p300-DY1399

    Plasmid
    #89095
    Purpose
    mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect
    Depositor
    Elizabeth Wilson
    Publication
    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p300-D1399Y mutant (EP300 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    p300-DY1399
    Promoter
    SV40
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    89095-plasmid-map-sequence-id-373226

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  37. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Publication
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80424-plasmid-map-sequence-id-151441

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  39. pcDNA5-FRT-TO-EGFP-AID

    Plasmid
    #80075
    Purpose
    Mammalian expression vector for N-terminal EGFP-AID tagging
    Depositor
    Andrew Holland
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-AID
    Tags
    AID and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80075-plasmid-map-sequence-id-150337

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Showing: 10801 - 10840 of 179521 results