170,806 results

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CAGGS-MCS-NLS-3XFLAG-V5

Plasmid

#196383

Purpose

Empty backbone vector to insert cDNA for strong expression in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Type

Empty backbone

Tags

3xFLAG and V5

Expression

Bacterial and Mammalian

Promoter

CAGGs

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Tubp-Gal80 in pCaSpeR4

Plasmid

#17748

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL80 (GAL80 Budding Yeast)

Tags

SV40 polyA and Tubulin 1alpha promoter

Expression

Insect

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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VCP F12.4 gRNA

Plasmid

#90939

Purpose

3rd generation lentiviral gRNA plasmid targeting human VCP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

VCP (Guide Designation F12.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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VCP F11.4 gRNA

Plasmid

#90938

Purpose

3rd generation lentiviral gRNA plasmid targeting human VCP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

VCP (Guide Designation F11.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pmEGFP_a_tubulin_C1

Plasmid

#21039

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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nontarget S. pyogenes scaffold CARGO array

Plasmid

#191319

Purpose

Contains an array of nontargeting guide RNAs

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6-FLAG

Plasmid

#30482

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-hGSDMB

Plasmid

#218936

Purpose

Expression of N-terminal FLAG tagged human gasdermin B

Depositor

Christopher LaRock

Article

LaRock et al Nature. 2022 May;605(7910):527-531. doi:

Insert

human GSDMB (GSDMB Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-eLACCO1.1

Plasmid

#167944

Purpose

Fluorescent biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLACCO1.1

Expression

Bacterial

Promoter

araBAD

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#101061

Purpose

AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2-F391W

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

Plasmid

#110854

Purpose

Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

sgRNA scaffold with spacer

Use

Lentiviral

Mutation

WT

Promoter

U6

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody (LaM-2)

Plasmid

#162276

Purpose

Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

mCherry-Nanobody (LaM-2)

Tags

GST

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A mPing

Plasmid

#145787

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock, Susan Wessler

Article

Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

Insert

mPing

Expression

Yeast

Mutation

WT

Available Since

Jan. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSMPP-mCherry-mTrim21

Plasmid

#104971

Purpose

Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (Trim21 Mouse)

Use

Lentiviral

Tags

mCherry

Promoter

SFFV

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRCA422 - pHR-UCOE-SFFV-VPH-dcas9-p2a-mCherry

Plasmid

#238165

Purpose

Express VPH-dCas9-P2A-mCherry in mammalian cells. Lentiviral

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

VPH-dCas9-P2A-mCherry

Use

CRISPR and Lentiviral

Tags

mCherry

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-Neo/Myc PD-L1

Plasmid

#121477

Purpose

expression of human Myc-PD-L1 in mamalian cell

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

Myc

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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WDV-Echistatin

Plasmid

#226985

Purpose

Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Inserts

WDV (repA )
Echistatin

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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WT (AF20)

Plasmid

#21359

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pET23b-15F11-HA-mEGFP-6xHis

Plasmid

#129593

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2 Flag-JunB

Plasmid

#29687

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

jun B oncogene (JUNB Human)

Tags

Flag

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a

Plasmid

#177435

Purpose

To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.

Depositor

David Andrews

Insert

Phosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-NEUROD1

Plasmid

#215031

Purpose

Mammalian expression of human NEUROD1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

neuronal differentiation 1 (NEUROD1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-NBR1

Plasmid

#38283

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Next to BRCA1 gene 1 protein (NBR1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA5/tom20-pbira(79-c)-ha

Plasmid

#154456

Purpose

expresses tom20-pbira(79-c)-ha/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

tom20-pbira(79-c)-ha (TOMM20 Human)

Expression

Mammalian

Promoter

CMV-F

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCox8a-mScarlet3-H (aka mYongHong)

Plasmid

#239410

Purpose

Expresses Cox8a-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

Cox8a (COX8A Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hnRNPU-V5

Plasmid

#35974

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)

Tags

V5

Expression

Mammalian

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-i_N1

Plasmid

#85056

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
iCAP-ROOT

Plasmid

#192262

Purpose

AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

Depositor

Li Ye

Article

Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

Insert

Synthetic construct for ROOT VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLX_lenti_osTir1_9Myc_P2A_Bsd

Plasmid

#139746

Purpose

lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

Depositor

Robert Tjian

Article

Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

Insert

rice E3 ligase Tir1 (osTir1)

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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PB533-44B12m23-sfGFP

Plasmid

#186778

Purpose

RNAP2 Ser5ph-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

44B12m23-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

Plasmid

#217306

Purpose

sgRNA mcherry + Puromycin

Depositor

Luke Gilbert

Insert

pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry

Use

CRISPR

Promoter

U6, Ef1alpha

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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TARDBP

Plasmid

#156073

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TARDBP (TARDBP Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

Plasmid

#176270

Purpose

Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

TadA-TadA*(8e)-dCasMINI-V4

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-Neo-ISG15

Plasmid

#80404

Purpose

overexpression of ISG15

Depositor

Isidoro Martinez

Article

Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

Insert

ISG15 (ISG15 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFPmAID

Plasmid

#174213

Purpose

GFP::mAID donor backbone

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Type

Empty backbone

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-GABARAP WT

Plasmid

#123097

Purpose

Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF3015

Plasmid

#144491

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PPARA (PPARA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pVax1-hVEGF165

Plasmid

#74466

Purpose

expression of hVEGF165 in mammalian cells

Depositor

Loree Heller

Article

Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

Insert

vascular endothelial growth factor 165 (VEGFA Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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170,806 results