165,472 results

1. pcDNA3 2xSTREP Cyclin F(LP35/36AA)

   Plasmid

   #236467

   Purpose

   transient overexpression of Cyclin F in mammalian cells

   Depositor

   Michele Pagano

   Article

   D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

   Insert

   Cyclin F (CCNF Human)
   

   Tags

   2xSTREP

   Expression

   Mammalian

   Mutation

   (LP35/36AA) first two amino acids of the F-box do…

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-0.5Syn-D2GFP-WPRE

   Plasmid

   #233060

   Purpose

   To express D2GFP from a 0.5 bp synapsin promoter.

   Depositor

   Jonathan Ploski

   Insert

   D2GFP
   

   Use

   Lentiviral

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971)

   Plasmid

   #231721

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   POLR2A (POLR2A Human)
   

   Tags

   Flag-GST-3C

   Expression

   Insect

   Mutation

   RPB1:1586-1971

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNX24-RFP-MS2SL

   Plasmid

   #227819

   Purpose

   Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Inserts

   3xHA-RFP-24xMS2SL
   Neo resistance
   

   Expression

   Mammalian

   Promoter

   CMV and SV40

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGEN-Dre-nls (JDW 1131)

   Plasmid

   #229809

   Purpose

   A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   DRE recombinase-nls
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP13030 - pAAV-AiE0462m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3030)

   Plasmid

   #229909

   Purpose

   AiE0462m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

   Plasmid

   #213527

   Purpose

   Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   D90A and E92A

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSF_his6_sumo_OC43_NSP14

   Plasmid

   #213519

   Purpose

   Expresses NSP14 of hCoV OC43

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV OC43 NSP14 (ORF1ab hCoV OC43)
   

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF_his6_sumo_NL63_NSP14

   Plasmid

   #213520

   Purpose

   Expresses NSP14 of hCoV NL63

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV NL63 NSP14 (ORF1ab hCoV NL63)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF_his6_sumo_229E_NSP14

    Plasmid

    #213521

    Purpose

    Expresses NSP14 of hCoV 229E

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV 229E NSP14
    

    Tags

    His6-sumo

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS416-ADH1p-TID-FKBP-HA3

    Plasmid

    #227998

    Purpose

    Expresses TurboID-FKBP fusion from ADH1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Tags

    HA tag

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUdOm2.1

    Plasmid

    #210278

    Purpose

    Empty backbone with ampicillin resistance for high expression in mammalian cells

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK484 (2mAID-Hygro)

    Plasmid

    #214396

    Purpose

    2mAID for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    2mAID-Hygro
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK487 (Hygro-P2A-3mAID)

    Plasmid

    #214399

    Purpose

    3mAID for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-3mAID
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK450 (BromoTag-mCherry-BSD)

    Plasmid

    #214380

    Purpose

    BromoTag-mCherry for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-mCherry-BSD
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

    Plasmid

    #169826

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A S1859A; TCCC -> AGTC silent mutat…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRAR074-control-sfGFP

    Plasmid

    #185032

    Purpose

    J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Expression

    Bacterial

    Mutation

    Nucleotides of rpmE 5'UTR scrambled, Only fi…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. psiCHECK2-miR-503-5p

    Plasmid

    #135725

    Purpose

    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-503-5p)

    Depositor

    Florian Karreth

    Insert

    miR-503-5p reverse complement
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWZL Neo Myr Flag VRK3

    Plasmid

    #20664

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    VRK3 (VRK3 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag

    Plasmid

    #231591

    Purpose

    Express circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-2xALFATag-10xSunTag
    

    Expression

    Mammalian

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pUB6-HuMGF

    Plasmid

    #190782

    Purpose

    Expresses membrane-bound form of human SCF in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

    Insert

    human stem cell factor (KITLG Human)
    

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET His6 MBP TEV LIC cloning vector (2M-T)

    Plasmid

    #29708

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. VAMP3-mCherry

    Plasmid

    #92423

    Purpose

    Expression of VAMP3 C-terminally conjugated to mCherry.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    VAMP3 (Vamp3 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. CMV500 A-C/EBPβ

    Plasmid

    #33363

    Depositor

    Charles Vinson

    Insert

    Cebpb (Cebpb Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see comments)

    Promoter

    CMV

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pMRXIP GFP-Stx18

    Plasmid

    #45918

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    syntaxin 18 (STX18 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

26. gTBEv5

    Plasmid

    #220621

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. mKeima-Red-C1

    Plasmid

    #54546

    Purpose

    Localization: C1 Cloning Vector, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mKeima-Red

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. pOPINK-OTUD3 (OTU, aa 52-209)

    Plasmid

    #61412

    Purpose

    Expresses human OTUD3 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 210-398.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAAV-R26-FLEX-mStr

    Plasmid

    #135619

    Purpose

    Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    CAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV2-CAG-3xNLS-mScarlet

    Plasmid

    #191098

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-mScarlet
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    Optimized to human codon usage

    Promoter

    CMV enhancer and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. pMXS-GshF-blasticidin

    Plasmid

    #210313

    Purpose

    Expresses humanized Streptococcus thermophilus GshF in mammalian cells

    Depositor

    Kivanc Birsoy

    Article

    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

    Insert

    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    

    Use

    Retroviral

    Tags

    FLAG peptide

    Mutation

    Codon optimized for expression in human cells

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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32. mRNA-CleanCap-NanoLuc

    Plasmid

    #188247

    Purpose

    Template plasmid to transcribe mRNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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33. pLKO-MCS

    Plasmid

    #185594

    Purpose

    Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLVX-opto-mCasp8

    Plasmid

    #208782

    Purpose

    Inducibly expresses mouse caspase8 fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mCaspase8 Cry2 (Casp8 Mouse)
    

    Use

    Lentiviral

    Tags

    Cry2 and mCherry

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCIP-AMPKa1_KD

    Plasmid

    #79011

    Purpose

    For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Expression

    Mammalian

    Mutation

    K47R

    Promoter

    pCAGGs

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 2Bc-T MBP_BoMoC(ed)_6xH

    Plasmid

    #186461

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

    Insert

    B. mori truncated R2 RT
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSIQ-EGFP

    Plasmid

    #216239

    Purpose

    All-in One IQ transgene switch for EGFP expression

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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39. pBAD33-Gm

    Plasmid

    #65098

    Purpose

    bacterial expression vector with Gentamycin selection, based on pBAD33

    Depositor

    Juan Tomas

    Article

    Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. hBax C3-EGFP

    Plasmid

    #19741

    Depositor

    Richard Youle

    Article

    Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

    Insert

    Bax (BAX Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge