20,202 results

Showing: 10801 - 10840 of 20202 results

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pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

Plasmid

#126679

Purpose

Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

Depositor

Hee Won Yang

Article

Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

Insert

Rb (a.a 886-928)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC 1-2644

Plasmid

#16511

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Expression

Mammalian

Mutation

Codon 2644 truncation

Available Since

April 30, 2008

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A WT

Plasmid

#69813

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 raptor

Plasmid

#21339

Depositor

David Sabatini

Article

Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

Insert

raptor shRNA 1 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 7, 2009

Availability

Academic Institutions and Nonprofits only
pCI-SEP-GluR2(Q)

Plasmid

#24002

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

GluR2 (Gria2 Rat)

Tags

GluR2 signal peptide (aa1-24) and SEP (supereclip…

Expression

Mammalian

Mutation

R607Q

Promoter

CMV

Available Since

April 6, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP36

Plasmid

#22579

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP36 (USP36 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-3xFLAG-hphMX4

Plasmid

#20755

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-ASAP3Kv-WPRE

Plasmid

#132330

Purpose

expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3KV

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-p300-DY1399

Plasmid

#89095

Purpose

mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300-D1399Y mutant (EP300 Human)

Tags

HA

Expression

Mammalian

Mutation

p300-DY1399

Promoter

SV40

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ114

Plasmid

#85995

Purpose

One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified bovine U6-2

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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ADORA1-Tango

Plasmid

#66209

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ADORA1 (ADORA1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pCW-LIC

Plasmid

#26098

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

Three consecutive Taq promoters, 2 active

Expression

Bacterial

Available Since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pSin-EF2-Oct4-Pur

Plasmid

#16579

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-DEST

Plasmid

#119745

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)

Depositor

Andrew Jackson, Martin Reijns

Article

Benitez-Guijarro et al EMBO J. 2018 Aug 1;37(15). pii: embj.201

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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psPAX2-D64V-NC-PP7

Plasmid

#122945

Purpose

PP7 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an PP7 aptamer into lentivirus-like particles

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

NC-PCP (PP7 coat protein) fusion protein in Gag

Use

Lentiviral

Expression

Mammalian

Mutation

D64V

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDicAID_nCas9-PmCDA_NptII_Della

Plasmid

#91694

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-B-GECO1

Plasmid

#32448

Purpose

Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Expression

Mammalian

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Promoter

CMV

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-Cyto-CaNARi

Plasmid

#64849

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Tags

Cerulean, Nuclear export signal (NES), and Venus

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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GAK-pmCherryN1

Plasmid

#27695

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

GAK (Gak Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pHR-FKBP:mCherry-Rab5a

Plasmid

#72901

Purpose

Early endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab5a (RAB5A Human)

Use

Lentiviral

Tags

FKBP(F36V) and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-215

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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coel::RFP

Plasmid

#8938

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Tags

RFP

Expression

Worm

Available Since

Nov. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pQE60-EFG-His

Plasmid

#124126

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor G (fusA Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET-His-SCov2-nsp7

Plasmid

#154757

Purpose

Expression of SARS-CoV-2 nsp7

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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lenti F9-TetR-EGFP-IRES-PuroR

Plasmid

#117049

Purpose

lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Insert

F9-TetR-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

F9 promoter

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBOT563

Plasmid

#40864

Depositor

Harold Gainer

Article

Fields et al PLoS One. 2012;7(2):e32085. Epub 2012 Fe

Insert

Oxytocin promoter

Use

AAV

Expression

Bacterial and Insect

Promoter

mouse oxytocin promoter

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Clathrin-mGreenLantern

Plasmid

#164462

Purpose

Localization of mGreenLantern fluorescent protein to clathrin

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Clathrin

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-TSC2

Plasmid

#15478

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

TSC2 shRNA (TSC2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
hOT-GFP

Plasmid

#67848

Purpose

mammalian expression of human oxytocin receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

oxytocin receptor (OXTR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pDD143

Plasmid

#119880

Purpose

Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_dCas9sth1

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
V247 pCEP 4HA B56gamma1

Plasmid

#14534

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5C B56 gamma1 regulatory subunit of PP2A (PPP2R5C Human)

Tags

4 HA

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Green Glifon4000

Plasmid

#126208

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon4000

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-LEU-GFP-VHL

Plasmid

#21053

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3 Paf1

Plasmid

#11059

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Paf1 (PAF1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pOpen-P45

Plasmid

#165570

Purpose

Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

P45

Use

Synthetic Biology

Available Since

Aug. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AAV-FLEX-rev-ChR2(H134R)-mCherry

Plasmid

#18916

Purpose

Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Has Service

AAV1

Insert

Channelrhodopsin 2-mCherry

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

mCherry

Mutation

H134R

Promoter

CAG

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-ATXN3

Plasmid

#22538

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

ATXN3 (ATXN3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
CBFRE-EGFP

Plasmid

#17705

Depositor

Nicholas Gaiano

Article

Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Insert

CBFRE

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBtet-RN

Plasmid

#60503

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcdna3 flag - ULK1 wt

Plasmid

#27636

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse ULK1 (Ulk1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

Initial M from ULK1 removed.

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-HA-rat Nrxn1alpha

Plasmid

#58266

Purpose

Expression plasmid for HA-tagged rat Neurexin 1alpha

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1alpha (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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20,202 results