We narrowed to 22,837 results for: Sis

1. pQZ083

   Plasmid

   #106417

   Purpose

   Express Complexin-1 fragment (1-83) in E. coli

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

   Insert

   complexin-1 fragment 1-83 (Cplx1 Synthetic, Rat)
   

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Fragment 1-83, Codon optimization

   Promoter

   T7

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSuper mouse DGCR8-2

   Plasmid

   #14773

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   DGCR8 shRNA (Dgcr8 Mouse)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pSicoR human Drosha3

   Plasmid

   #14768

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   Drosha shRNA (DROSHA Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pJK503

   Plasmid

   #155380

   Purpose

   dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Available Since

   May 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-Flag-hsDicer (D1320A)

   Plasmid

   #41586

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D1320A

   Promoter

   CMV

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pHH0103_SNX9-1/1

   Plasmid

   #91513

   Purpose

   Protein expression and purification of human SH3 domain construct SNX9-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SNX9-1/1 (SNX9 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-NLS-FRB-VP16

   Plasmid

   #210515

   Purpose

   expresses NLS-FRB-VP16 component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NLS-FRB-VP16
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJMP1069

   Plasmid

   #119244

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

   Plasmid

   #136646

   Purpose

   Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

   Depositor

   Maria Balda, Karl Matter

   Article

   Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

   Insert

   SH3BP1R312A (RNAi resistant) (SH3BP1 Human)
   

   Tags

   HA (MQDLPGNDNSTAG)

   Expression

   Mammalian

   Mutation

   GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDendra2-Hygro

    Plasmid

    #202407

    Purpose

    Dendra2 expression plasmid

    Depositor

    Morito Kurata

    Article

    Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

    Insert

    Hygromycin
    

    Tags

    Dendra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. NELFE-mOrange pHis-//

    Plasmid

    #53303

    Purpose

    Expresses His-tagged NELF-E-mOrange fusion protein.

    Depositor

    John Lis

    Article

    Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

    Insert

    NELF-E protein (Nelf-E Fly)
    

    Tags

    His and mOrange

    Expression

    Bacterial

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAd FLAG HDAC5 WT

    Plasmid

    #32209

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pGWB419

    Plasmid

    #74813

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MDH1-PGK-GFP-miR9

    Plasmid

    #25036

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

    Insert

    mir-9-3 (MIR9-3 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCaSpeR4 mito roGFP2-Orp1

    Plasmid

    #65001

    Purpose

    Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-HA-hTRAF3 333-C

    Plasmid

    #44035

    Depositor

    Shao-Cong Sun

    Article

    Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

    Insert

    TNF receptor-associated factor 3 (TRAF3 Human)
    

    Tags

    1xHA

    Expression

    Mammalian

    Mutation

    Contains amino acids 334-568; S393G and K492N

    Available Since

    April 4, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Psicheck2 Sox4 full length 3'UTR

    Plasmid

    #26989

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    Sox4 Full length 3' UTR (SOX4 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pLL3.7 shRNA against CRTC2

    Plasmid

    #72711

    Purpose

    Lentiviral expression of shRNA against CRTC2 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC2
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pTRE-FDXR wt

    Plasmid

    #16610

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    FDXR (FDXR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGWB522

    Plasmid

    #74864

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLentiCRISPRv1.mir451_KO

    Plasmid

    #164689

    Purpose

    For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    miR-451 CRISPR KO gRNA (MIR451A )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCS2mFoxd3

    Plasmid

    #37267

    Depositor

    Patricia Labosky

    Article

    Steiner et al Development. 2006 Dec;133(24):4827-38. E

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    Overexpression

    Expression

    Mammalian

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pHH0103_MYO15A-1/2

    Plasmid

    #91491

    Purpose

    Protein expression and purification of human SH3 domain construct MYO15A-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MYO15A-1/2 (MYO15A Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJMP1161

    Plasmid

    #119251

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

    Plasmid

    #108375

    Purpose

    FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMRX-IP/SECFP-mDFCP1

    Plasmid

    #58765

    Purpose

    Expresses SECFP-mDFCP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    SECFP

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

27. p6903 PHAGE-B CMV-N-EGFP Myc

    Plasmid

    #37608

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    Myc (MYC Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pCDH-hSyn-IntCCCre-T2A-CopGFP

    Plasmid

    #160503

    Purpose

    Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLexm-gfp-Pept2

    Plasmid

    #167988

    Purpose

    expresses rat PepT2 with a c terminal cleavable GFP-His tag.

    Depositor

    Simon Newstead

    Article

    Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

    Insert

    PepT2 (Slc15a2 Rat)
    

    Tags

    cleavable GFP-his8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

    Plasmid

    #127360

    Purpose

    gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Mutation

    R118G

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p156 pWZL-EGFP-SLPI

    Plasmid

    #11069

    Depositor

    Ramesh Shivdasani

    Article

    Schulze et al Blood. 2004 Dec 15. 104(13):3949-57.

    Insert

    SLPI (Slpi Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

32. pJMP1071

    Plasmid

    #119245

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET23b-Casp7 C186A-His

    Plasmid

    #11826

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 7 catalytic mutant (CASP7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C186A

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pHH0103_VAV2-2/2

    Plasmid

    #91506

    Purpose

    Protein expression and purification of human SH3 domain construct VAV2-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV2-2/2 (VAV2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. optoPKAW196R

    Plasmid

    #104548

    Purpose

    Expresses Cry2-mCherry-PKA W196R in mammalian cells

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Cry2-mCherry-PKA W196R
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    PKA W196R

    Promoter

    CMV

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. UKNP4.3.2

    Plasmid

    #98270

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. UKNP3.2.3

    Plasmid

    #98248

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. UKNP3.1.1

    Plasmid

    #98214

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPKm-102

    Plasmid

    #90493

    Purpose

    pcDNA3 - CMVmin - mOrange, plasmid expressing orange fluorescent protein (OFP)

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mOrange
    

    Expression

    Mammalian

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LentiU6-hNEUROD1 gRNA_1-MS2-Puro

    Plasmid

    #192676

    Purpose

    Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human NEUROD1 activating gRNA #1 (NEUROD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge