19,593 results

1. M51 Super 8x FOPFlash (TOPFlash mutant)

   Plasmid

   #12457

   Purpose

   Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   mutant TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   mutant TCF/LEF binding sites

   Available Since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. enAsABE8e

   Plasmid

   #138506

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-dAsCas12a (En)
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. nls-EGFP

   Plasmid

   #67652

   Purpose

   Mammalian expression vector driving nls-EGFP.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   nls-EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCS2+Cas9-mSA

   Plasmid

   #103882

   Purpose

   Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   CAS9-MSA
   

   Use

   CRISPR

   Tags

   MSA (monomeric streptavidin

   Mutation

   none

   Promoter

   SP6

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVTNT-T7-KPNA6

   Plasmid

   #26682

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 6 (KPNA6 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Mm.cargo(Cas9)

   Plasmid

   #174865

   Purpose

   Expresses mouse Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by MmPeg10 UTRs
   

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

   Plasmid

   #221899

   Purpose

   Expresses alpha tubulin fused to mStayGold

   Depositor

   Janelia Integrative Imaging

   Insert

   mStayGold-aTubulin
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiGuide-Hygro

   Plasmid

   #139462

   Purpose

   Lentiviral vector with gRNA scaffold and hygromycin selectable marker

   Depositor

   Caroline Goujon

   Insert

   no sgRNA inserted; resistance gene: hygroR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-mCherry

   Plasmid

   #50462

   Purpose

   Double floxed mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   mCherry
   

   Use

   AAV

   Tags

   N/A

   Promoter

   EF1a

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRE recombinase

    Plasmid

    #62730

    Purpose

    Expresses Cre recombinase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. FRB-mCherry-FTH1

    Plasmid

    #100749

    Purpose

    Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

    Depositor

    Stephen Royle

    Article

    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

    Insert

    ferritin heavy chain 1 (FTH1 Human)
    

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSIN-TRE-H2BeGFP-rtTA2

    Plasmid

    #165494

    Purpose

    Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

    Depositor

    Hector G. Palmer

    Article

    Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

    Insert

    Human histone H2B (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Mutation

    -

    Promoter

    TetO-CMV

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-mRFP

    Plasmid

    #13032

    Purpose

    Mammalian expression vector for cloning your gene fused to mRFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Monomer Red Fluorescent Protein
    

    Expression

    Mammalian

    Available Since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

    Plasmid

    #176268

    Purpose

    Expression of dCas12f-VPR-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    dCas12f
    

    Use

    Lentiviral

    Tags

    HA-VPR-mCherry

    Expression

    Mammalian

    Mutation

    D326A, D510A

    Promoter

    PGK

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTNT-B18R-6His

    Plasmid

    #58979

    Purpose

    expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    B18R
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7/Sp6

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6.2_mTagBFP2

    Plasmid

    #113725

    Purpose

    Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Article

    Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

    Insert

    mTagBFP2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-15F11-HA-mCh

    Plasmid

    #129591

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXPR_120

    Plasmid

    #96917

    Purpose

    for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-FOXP1

    Plasmid

    #153145

    Purpose

    Expression of Flag tagged human FOXP1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXP1 (FOXP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENN.AAV.hSyn.Cre.WPRE.hGH

    Plasmid

    #105553

    Purpose

    AAV expression of Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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