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Showing: 541 - 560 of 19022 results

  2. pAD-GamS

    Plasmid
    #99246
    Purpose
    Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    gamS (with C-terminal 6xHis tag)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99246-plasmid-map-sequence-id-339990

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  5. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

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  6. GLP1R-Tango

    Plasmid
    #66295
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GLP1R (GLP1R Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66295-plasmid-map-sequence-id-115875

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  8. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161928
    Purpose
    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    Surface marker and Citrine activation reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161928-plasmid-map-sequence-id-325287

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  9. pAdTrack-CMV

    Plasmid
    #16405
    Purpose
    Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.
    Depositor
    Bert Vogelstein
    Article
    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2008
    Availability
    Academic Institutions and Nonprofits only
    16405-plasmid-map-sequence-id-190476

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  10. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Article
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Use
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    123461-plasmid-map-sequence-id-417805

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  13. TNFR1-YFP

    Plasmid
    #111209
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TNFRSF1A (TNFRSF1A Human)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111209-plasmid-map-sequence-id-212987

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  15. pcDNA3.1-hNurr1-NE

    Plasmid
    #102363
    Purpose
    Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells
    Depositor
    Shu Leong Ho
    Insert
    NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
    Use
    Tags
    NE
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    102363-plasmid-map-sequence-id-200857

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  17. Lenti-iCas9-neo

    Plasmid
    #85400
    Purpose
    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Insert
    Flag-iCas9-P2A-GFP
    Use
    Lentiviral
    Tags
    Flag-iCas9-P2A-GFP
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    85400-plasmid-map-sequence-id-429030

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Showing: 541 - 560 of 19022 results