8,806 results

1. PB-TO-ASCL1-DLX2

   Plasmid

   #182307

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   Ascl1 (Ascl1 Mouse)
   Dlx2 (Dlx2 Mouse)
   puroR-T2A-mycNLS-mTagBFP2
   

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Promoter

   EF1a and pTRE3G-bidirectional

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PE2-SpRY

   Plasmid

   #159979

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmGFP-ADAR1-p110

   Plasmid

   #117928

   Purpose

   ADAR1-p110 expression plasmid.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Insert

   ADAR1-p110 (ADAR Human)
   

   Tags

   myc-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-hALIX

   Plasmid

   #21504

   Depositor

   James Hurley

   Article

   Lee et al Science. 2008 Oct 24. 322(5901):576-80.

   Insert

   ALIX (PDCD6IP Human)
   

   Tags

   Cherry

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

   Plasmid

   #141395

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-MMLVgag-3xNES-ABE8e

   Plasmid

   #181751

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE puro human IRS-1

   Plasmid

   #11025

   Depositor

   Ronald Kahn

   Article

   Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

   Insert

   IRS-1 (IRS1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-IRES-Puro

   Plasmid

   #85132

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti PGK V5-LUC Puro (w543-1)

   Plasmid

   #19360

   Purpose

   3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUdeltaGW-rtTA

    Plasmid

    #19780

    Purpose

    3rd generation lentiviral vector

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    reverse tetracycline transactivator
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL11024 (pICH47732::NOSp-NPTII-OCST)

    Plasmid

    #51144

    Purpose

    Confers KAN resistance in planta

    Depositor

    Jonathan D Jones

    Insert

    NOSp-NPTII-OCST
    

    Available Since

    Feb. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. YE1-BE4max

    Plasmid

    #138155

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9-UGI-UGI
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-NLRC5

    Plasmid

    #37521

    Depositor

    Thomas Kufer

    Article

    Neerincx et al J Biol Chem. 2010 Aug 20;285(34):26223-3

    Insert

    NLRC5 (NLRC5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS2M-PH-gagpol-D64V

    Plasmid

    #166031

    Purpose

    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

    Insert

    MS2-Gag-Pol
    

    Use

    Lentiviral

    Mutation

    D64V mutation within the integrase within Pol

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. phND2-N174

    Plasmid

    #31822

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    NeuroD2 (NEUROD2 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-nCas9n

    Plasmid

    #47929

    Purpose

    expression of an optimized Cas9 for genome-editing in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    nls-zcas9-nls
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-AML1/ETO-IRES-GFP

    Plasmid

    #60832

    Purpose

    AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    AML1/ETO (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-PML IV/pRK5

    Plasmid

    #59742

    Purpose

    Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

    Depositor

    Xiaolu Yang

    Article

    Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

    Insert

    PML isoform IV (PML Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Inducible Caspex expression

    Plasmid

    #97421

    Purpose

    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

    Depositor

    Samuel Myers, Steven Carr

    Article

    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

    Inserts

    Caspex
    T2A-Gfp
    

    Use

    CRISPR

    Tags

    3xFLAG and V5

    Expression

    Mammalian

    Mutation

    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-CMV-jGCaMP7f

    Plasmid

    #104483

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7f
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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