59,428 results

1. pT7-hMLH1dn for IVT

   Plasmid

   #178114

   Purpose

   Template for in vitro transcription of human MLH1dn

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (codon optimized) (MLH1 Human)
   

   Use

   Template for in vitro transcription

   Mutation

   Detailed in manuscript

   Promoter

   T7 (inactivated)

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ALDH18A1

   Plasmid

   #155402

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   ALDH18A1 (ALDH18A1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUGW-Cytosol-TurboID

   Plasmid

   #200961

   Purpose

   3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   Cytosol-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.22-VEGF-HRE::dLUC

   Plasmid

   #128096

   Purpose

   Hypoxia response element real-time luciferase reporter

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
   

   Use

   Luciferase

   Tags

   drives expression of destabilized luciferase (2CP…

   Expression

   Mammalian

   Promoter

   inserted VEGF hypoxia response elements (HRE) (x5)

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Human Myc-XIAP

   Plasmid

   #217627

   Purpose

   Mammalian expression of Myc tagged XIAP

   Depositor

   Ethan Lee

   Article

   Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

   Insert

   XIAP (XIAP Human)
   

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJet-CMV-IARS1-GFP

   Plasmid

   #212322

   Purpose

   EGFP-tagged IARS1

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   IARS (IARS1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   CMV

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

   Plasmid

   #48795

   Purpose

   expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Chen et al Cell. 2003 May 16;113(4):457-68.

   Inserts

   YWHAE (YWHAE Human)
   YWHAZ (YWHAZ Human)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Promoter

   CMV and CMV (IRES)

   Available Since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-GRAB-gDA3m

   Plasmid

   #208707

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_gDA3m
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZL hygro Flag HA TRAP220 wt

    Plasmid

    #17433

    Purpose

    Retroviral expression of Med1/TRAP220 in mammalian cells

    Depositor

    Kai Ge

    Article

    Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

    Insert

    TRAP220 (MED1 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPGK-T7/2-CD44v9 (fl)

    Plasmid

    #137818

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v9 (fl) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and mutation H48L

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUGW-ER-TurboID

    Plasmid

    #200965

    Purpose

    3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    ER-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-CSNK1D

    Plasmid

    #23796

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CSNK1D (CSNK1D Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3 HA human NEMO

    Plasmid

    #13512

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    NEMO (IKBKG Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF1652

    Plasmid

    #143532

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSERT pcDNA3

    Plasmid

    #15483

    Depositor

    Randy Blakely

    Article

    Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

    Insert

    hSERT (SLC6A4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6/V5/DEST-VPS35

    Plasmid

    #21691

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Vacuolar Protein Sorting 35 homolog (VPS35 Human)
    

    Use

    Lentiviral; Invitrogen gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-myc-CUL3

    Plasmid

    #19893

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 3 (CUL3 Human)
    

    Tags

    3xmyc

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-IRES-GFP-human-Caspase-4

    Plasmid

    #183356

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Promoter

    MESV

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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