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pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX

Plasmid

#208692

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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proE-cad178-Luc

Plasmid

#42081

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -178 to …

Promoter

E-cadherin

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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proE-cad178-Luc-mEbox

Plasmid

#42082

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -178 to …

Promoter

E-cadherin

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pDY1622 NOLC1 sgRNA 1

Plasmid

#234833

Purpose

sgRNA 1 for NOLC1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

NOLC1 sgRNA (NOLC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) NAPstar3b

Plasmid

#232736

Purpose

Expresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar3b

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Mito-7

Plasmid

#55102

Purpose

Localization: Mitochondria, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Mito (COX8A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-XBP1 mNeonGreen NLS

Plasmid

#115968

Purpose

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

X-box binding protein 1 (XBP1 Synthetic, Human)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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FRESCA-syntide

Plasmid

#132415

Purpose

FRET-based sensor for CaMKII activity

Depositor

Margaret Stratton

Article

Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

Insert

FRESCA-syntide

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IFNG-Fc(DAPA)-AviTag-6xHis

Plasmid

#156535

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IFNG (IFNG Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pKeima-LiveDrop

Plasmid

#199695

Purpose

lipophagy reporter system based on the Keima-fluorophore

Depositor

Tobias Walther

Article

Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

Insert

GPAT4 (GPAT4 Human)

Use

Tags

Keima

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-dSV40-CTLA-4-mGFP

Plasmid

#223594

Purpose

Lentiviral expression of human CTLA4 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-mCherry

Plasmid

#20972

Purpose

H2B fused to mCherry for chromatin visualization

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-TMEM192-mKeima

Plasmid

#215358

Purpose

Probe to evaluate lysophagic activity

Depositor

Tamotsu Yoshimori

Article

Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

Insert

transmembrane protein 192 (TMEM192 Human)

Use

Retroviral

Tags

mKeima

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGL2-p21 promoter-Luc

Plasmid

#33021

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

p21 promoter (CDKN1A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

p21

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only

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lentiMPH v2

Plasmid

#89308

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

Depositor

Feng Zhang

Article

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Insert

MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-alphasynuclein-A53T

Plasmid

#40823

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

A53T (aggregates more rapidly than the wild-type …

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-DDB1

Plasmid

#19918

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-Cre-blast

Plasmid

#179390

Purpose

Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

Insert

mCherry (P2A) Cre recombinase

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcs2-ATF4uORF1-2-GFP-IRES-mCherry

Plasmid

#226105

Purpose

ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

atf4 (ATF4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains uORF1-2 of ATF4, whose stability can ser…

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hAtg2A

Plasmid

#36456

Depositor

Noboru Mizushima

Article

Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

Insert

autophagy-related protein 2 homolog A (ATG2A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

changed Proline 656 to Arginine

Promoter

CMV

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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