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8,969 results

Showing: 8461 - 8490 of 8969 results

  2. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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  23. L4417

    Plasmid
    #1649
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    RNAi
    Tags
    T7p, gfpC, gfpS65S, gfpN, T7p, lacZN, OriF1>&g…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1649-plasmid-map-sequence-id-259

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  28. pFA6a-link-tdTomato-SpHis5

    Plasmid
    #44640
    Purpose
    Yeast tagging vector to create gene fusion with tdTomato with SpHis5 selection
    Depositor
    Kurt Thorn
    Insert
    tdTomato
    Expression
    Yeast
    Available Since
    May 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44640-plasmid-map-sequence-id-61108

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Showing: 8461 - 8490 of 8969 results