We narrowed to 10,745 results for: AGA

1. pCas2B

   Plasmid

   #82373

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas2A

   Plasmid

   #82372

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-EGFP-RORbshRNA1

   Plasmid

   #73985

   Purpose

   RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse RORb gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAG-mCherry-RORbshRNA1

   Plasmid

   #73983

   Purpose

   RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse RORb gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPR - C3orf17 sgRNA 2

   Plasmid

   #70653

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C3orf17
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pU6_(GLuc)_INT[S1Apt]

   Plasmid

   #68425

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct with S1 streptavidin aptamer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSicoR-ADAM21i1

   Plasmid

   #59604

   Purpose

   Expression of shRNA against human ADAM21

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   ADAM21 (ADAM21 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pSicoR-CENPBi2

   Plasmid

   #59619

   Purpose

   Expression of shRNA against human CENPB

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   CENPB (CENPB Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSicoR-SLC17A5i1

   Plasmid

   #59598

   Purpose

   Expression of shRNA against human SLC17A5

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   SLC17A5 (SLC17A5 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pSicoR-PTPRJi2

    Plasmid

    #59609

    Purpose

    Expression of shRNA against human PTPRJ

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    PTPRJ (PTPRJ Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pSicoR-SLC17A5i2

    Plasmid

    #59599

    Purpose

    Expression of shRNA against human SLC17A5

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    SLC17A5 (SLC17A5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

12. Cdk12_intron4_sg1_pX458

    Plasmid

    #127175

    Purpose

    sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cdk12 Intron 4 sgRNA
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6 Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKDsgRNA-NT

    Plasmid

    #89960

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    empty gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCF806-shRen.713

    Plasmid

    #186712

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Insert

    shRen.713
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pComb3XTT

    Plasmid

    #63891

    Purpose

    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    TT Fab
    

    Use

    Phage display

    Tags

    6xHis, HA tag, and geneIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR v2-sgControl

    Plasmid

    #125836

    Purpose

    KO control

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    Control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. sg2.02 16x(MS2) MUC4.1

    Plasmid

    #101154

    Purpose

    gRNA with 16 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET3a-OSF-CypA

    Plasmid

    #79039

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OneSTrEP-FLAG- Cyclophilin A (PPIA Human)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCA24N-p50-ligase

    Plasmid

    #87742

    Purpose

    IPTG-inducible expression of p50-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and human p50/NF-kB (amino acids 40-366)

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJYS3_ΔcrtYf

    Plasmid

    #85542

    Purpose

    Constitutive transcription of FnCpf1 and crRNA of crtYf

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX330-EN1201

    Plasmid

    #92144

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PECAM1-3'gRNA

    Plasmid

    #235606

    Purpose

    gRNA targeting PECAM1 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al Angiogenesis. 2025 Jan 3;28(1):10. doi:

    Insert

    PECAM1 gRNA (PECAM1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDECKO_GFP

    Plasmid

    #72619

    Purpose

    Expresses two gRNAs targeting GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pQCascade

    Plasmid

    #140622

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXPR_502_sgCD4

    Plasmid

    #158704

    Purpose

    CD4 CRISPRa control

    Depositor

    David Root, John Doench

    Article

    Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEF-Bos TRIF Flag

    Plasmid

    #41550

    Depositor

    Tom Maniatis, Kate Fitzgerald

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    Trif (TICAM1 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

28. LentiCRISPR v2 hBAX

    Plasmid

    #129580

    Purpose

    CRISPR deletion of human BAX

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs human BAX (BAX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCR1281

    Plasmid

    #111124

    Purpose

    Expresses XRCC3_+_104181786.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    XRCC3 gRNA (XRCC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-shRNA2-zsGreen-PGK-puro_scramble shRNA

    Plasmid

    #197991

    Purpose

    For the stable knockdown experiments using shRNA, scramble sequence was inserted as the control.

    Depositor

    Bo Wang

    Article

    Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

    Insert

    scramble shRNA (control)
    

    Use

    Lentiviral

    Tags

    zsGreen1

    Expression

    Mammalian

    Mutation

    15q11.2 deletion

    Promoter

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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