178,736 results
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Plasmid#149722PurposeLentiviral expression of crRNA with GFP and a neomycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by
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CMV-mito-Citron1
Plasmid#134305Purposemitochondria-targeted direct-response citrate sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCitron1
ExpressionMammalianPromoterCMVAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry
Plasmid#128327PurposeReporter to evaluate YAP1/TEAD-mediated gene transcriptionDepositorAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin
Plasmid#134860PurposeExpresses EGFP-tagged recycling endosome markerDepositorAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-(empty)-TAG
Plasmid#138209PurposeTo facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' regionDepositorTypeEmpty backboneUseT7 transcriptionTagsTAG sequenceExpressionMammalianPromoterCMV Promoter, T7 PromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBbAW4k-loxP-TT-loxP-mRFP1
Plasmid#134405PurposeFluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.DepositorInsertW4-loxP-TT-loxP-mRFP1
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterW4 (modified T7A1)Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE-60NA-msGFP2
Plasmid#135301PurposeEncodes a photostable monomeric superfolder GFPDepositorInsertmsGFP2
ExpressionBacterialPromoterT5Available sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag
Plasmid#132403Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059AAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pB-TAG-NICD
Plasmid#130934PurposePiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cellsDepositorAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSET-mEosEM
Plasmid#132708Purposeapplication in Correlative light-electron microscopyDepositorInsertmEosEM
ExpressionBacterialAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSFDuet-sumo-h-cGAS
Plasmid#127161Purposeexpression of human cGAS proteinDepositorAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDisplay-mgSrtA
Plasmid#125792PurposeTransfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmgSrtA
ExpressionMammalianAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCE046-SiT-Cas12a-[Repr]
Plasmid#128132PurposeExpress both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Repr]-Triplex
UseAAVExpressionMammalianAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
fwYellow chromoprotein
Plasmid#117841PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coliDepositorInsertpromoter, RBS, fwYellow
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Neo-PIP-NLS-mVenus
Plasmid#118619PurposeLentiviral expression of the cell cycle marker PIP-NLS-mVenusDepositorInsertPIP-NLS-mVenus
UseLentiviralTagsmVenusExpressionMammalianPromoterhPGKAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBLO 62.5
Plasmid#123124PurposeHuman expression vector for planctomyces CasX and sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCasX protein
CasX sgRNA
UseCRISPRExpressionMammalianAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CD40L-SrtA
Plasmid#121167PurposepMP71 vector expressing Tomato - P2A - CD40L - SrtA - flagDepositorInsertCD40L-SrtA
UseRetroviralTagsTomato and flagExpressionMammalianAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HIV-1 LTR-gfp
Plasmid#115809PurposeHIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vprDepositorInsertgfp and ngfr
UseLentiviralPromoterHIV1 LTRAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-fDIO-Cre
Plasmid#121675PurposeExpresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)DepositorHas serviceAAV1, AAV5, AAV8, AAV9, and AAV RetrogradeInsertNLS-CRE-HA
UseAAVTags3xHA and SV40-NLSExpressionMammalianPromoterEF1aAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pV1093
Plasmid#111428PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1DepositorPublicationInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTP288
Plasmid#113460Purposehuman Flag tagged Nbs1 in baculovirus transfer vectorDepositorAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
His6-SpyCatcher-mi3
Plasmid#113043PurposeExpresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertHis6-SpyCatcher-mi3
TagsHis-tagExpressionBacterialPromoterT7Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pALOD4
Plasmid#111026PurposeExpresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472ADepositorInsertDomain 4 of Anthrolysin O
TagsHis6 tagExpressionBacterialMutationMutations: S404C, C472APromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EFS-GFP
Plasmid#110834Purposepositive control for GFP expressionDepositorInsertelongation factor 1alpha binding sequence (EFS) at GFP promoter region
UseLentiviralExpressionMammalianAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
plenti-UBC-gate-3xHA-pGK-PUR
Plasmid#107393PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseGateway Cloning and LentiviralTags3xHAExpressionMammalianAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJM1 EGFP-Tau
Plasmid#108868PurposeExpresses EGFP linked to human TauDepositorInsertTau (MAPT Human)
UseLentiviralTagsEGFPExpressionMammalianMutationhuman four-repeat tau lacking the N-terminal sequ…PromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pECFP18aaEYFP
Plasmid#109330PurposeECFP-EYFP FRET positive controlDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertECFP-EYFP FRET positive control
ExpressionMammalianMutationECFP linked to EYFP with 18 aa linker GLRSRAQASNS…PromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZyxin-mMaple3
Plasmid#101151PurposeFluorescent protein for superresolution imaging and a focal adhesion protein for binding substrateDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized mMaple3 fused to zyxin
ExpressionMammalianPromotercmvAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-DIO-EYFP
Plasmid#104052PurposeAn AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoterDepositorHas serviceAAV PHP.V1InsertEYFP
UseAAVPromoterCAGAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by