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167,150 results

Showing: 11561 - 11600 of 167150 results

  1. CALCRL-Tango

    Plasmid
    #66234
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CALCRL (CALCRL Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66234-plasmid-map-sequence-id-347881

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  5. pAR18_StrepI_mFASm_H8_pET22b

    Plasmid
    #122847
    Purpose
    Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122847-plasmid-map-sequence-id-238541

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  9. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

    Plasmid
    #105448
    Purpose
    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector
    Depositor
    Hillel Adesnik
    Article
    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:
    Has Service
    AAV9
    Insert
    ChrimsonR-mRuby2-ST
    Use
    AAV
    Tags
    mRuby2 and soma targeting motif
    Mutation
    K176R in Chrimson
    Promoter
    hSyn
    Available Since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105448-plasmid-map-sequence-id-448714

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  10. iRFP-H2A

    Plasmid
    #158691
    Purpose
    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Insert
    iRFP670
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158691-plasmid-map-sequence-id-311047

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  12. pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234439
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234439-plasmid-map-sequence-id-465689

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  15. pZac2.1 gfaABC1D-cyto-GCaMP8f

    Plasmid
    #186050
    Purpose
    AAV-mediated expression of GCaMP8f under the control of GfaABC1D promoter
    Depositor
    Loren Looger
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186050-plasmid-map-sequence-id-370166

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  16. AICSDP-10:LMNB1-mEGFP

    Plasmid
    #87422
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87422-plasmid-map-sequence-id-173949

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  19. TFORF3165

    Plasmid
    #144641
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TRIM28 (TRIM28 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144641-plasmid-map-sequence-id-348484

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  26. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid
    #232929
    Purpose
    Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    OsTir1(F74G)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    changed Phenylalanine 74 to Glycine
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232929-plasmid-map-sequence-id-466497

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  31. pAcGP67A - murine TfR

    Plasmid
    #12392
    Depositor
    Pamela Bjorkman
    Insert
    transferrin receptor (Tfrc Mouse)
    Tags
    6X-His tag, Factor Xa cleavage site, and leader p…
    Expression
    Insect
    Mutation
    See comments section
    Available Since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    12392-plasmid-map-sequence-id-274275

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  32. pLV Rnf121-V5

    Plasmid
    #175130
    Purpose
    Lentiviral expression of mouse Rnf121-V5
    Depositor
    Larry Gerace
    Insert
    Rnf121 (Rnf121 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175130-plasmid-map-sequence-id-352014

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  33. HRH4-Tango

    Plasmid
    #66403
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HRH4 (HRH4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

    Plasmid
    #234952
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234952-plasmid-map-sequence-id-467733

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Showing: 11561 - 11600 of 167150 results