170,823 results
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Plasmid#191465PurposeGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
ExpressionMammalianAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1-Fc(IgG1)
Plasmid#145164PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
RUBYm2
Plasmid#232175PurposeIntroduce a C-to-T mutation to generate an early stop codon (Q139 to stop), thereby interrupting RUBY expressionDepositorInsertRUBY mutant2 and CaMV 35S promoter
ExpressionPlantAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
RUBY-ABE#2
Plasmid#232176PurposeT-DNA vector with ecTadA8e-zCas9D10A and corresponding sgRNA to correct RUBYm2 and recover a vivid red color visible to the naked eyes.DepositorInsert2x35s-ecTadA8e-SpCas9-D10A-AtU3-sgRNA-mis4-gRNA scaffold
UseCRISPRTags3xflag and NLSExpressionPlantAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Nrf2
Plasmid#67636PurposeAAV vector expressing Nrf2 geneDepositorAvailable SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST-eGFP-C1-VPS35
Plasmid#163622Purposehuman VPS35 cDNA C-terminally tagged with eGFPDepositorAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX333-T2A-EGFP
Plasmid#197417PurposeSpCas9-T2A-EGFP with dual sgRNA cloning backbonesDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCBh; U6Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9
Plasmid#184464PurposePlasmid encoding PRDM9-Cas9 fusion under CMV promoterDepositorInsertPRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
UseCRISPRTagsFLAGExpressionMammalianPromoterCMVAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV4E
Plasmid#224452PurposeRep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRGDFNNT insert between amino acids 588 and 589 of…Promoterp41Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hACE2
Plasmid#145033PurposeExpress human ACE2 with C9 tag at C-terminal in mammalian cellsDepositorAvailable SinceApril 15, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
YFP-S100A10
Plasmid#107200PurposeExpresses YFP-human S100A10 fusion protein for live cell imagingDepositorAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B2.0
Plasmid#99888PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_WT
Plasmid#98653PurposeExpresses MBP-tagged FUS AA 1-163DepositorAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK287 (mAID-Hygro)
Plasmid#72825PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Hygro
UseCRISPRTagsmAIDExpressionMammalianAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNFkB:eGFP
Plasmid#44922DepositorInsertEGFP
Available SinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidExpressionBacterialAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro
Plasmid#141385PurposeLentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-E (E SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable SinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCN38-yfp
Plasmid#227637PurposeNegative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertyfp
ExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSRKBB-empty
Plasmid#59449PurposeContains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.DepositorTypeEmpty backboneExpressionBacterialPromotermodified lacAvailable SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
TEV Protease-mCherry
Plasmid#243793PurposeEncodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.DepositorInsertTEV protease
ExpressionMammalianAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF2102
Plasmid#141923PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti_EGFP-P2A-HAUS6_IRES_Blast
Plasmid#182886PurposeTransfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (WT full length)DepositorInsertHAUS 6 (HAUS6 Human)
UseLentiviralTagsEGFPExpressionBacterial and MammalianPromoterEF1alpha coreAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBHt2
Plasmid#104175PurposeEmpty backbone for Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporumDepositorTypeEmpty backboneUseFungal expressionPromoternoneAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT1T2
Plasmid#50593PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, gRNA scaffold, OsU3t plus, TaU3p, T2
UseCRISPR; Pcr templateExpressionPlantAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lac-I-SceI-Tet
Plasmid#17655DepositorInsertlac I-SceI tet
ExpressionBacterialAvailable SinceMay 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Raptor GFP
Plasmid#112744Purposeexpress Raptor-GFPDepositorAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWBs00
Plasmid#222476PurposeMarchantia polymorpha transformation vector carrying sulfadiazine selection marker.DepositorTypeEmpty backboneExpressionPlantAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-HcKCR1-EYFP
Plasmid#182021PurposeExpress light-gated potassium channel HcKCR1-EYFP in mammalian cellsDepositorInsertHcKCR1
UseAAVTagsEYFPExpressionMammalianAvailable SinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-cIAP1/pRK5
Plasmid#27972DepositorAvailable SinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSP4-bio
Plasmid#47717PurposeExpresses enzymatically monobiotinylated full-length MSP4 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP4
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163029PurposeFLiCRE TF for neuronal expressionDepositorInsertNrxn3b-MKII-hLOV1-TEVcs-tTA
UseAAVExpressionMammalianPromoterSynapsinAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_23C
Plasmid#91140PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0991
Plasmid#142240PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-vhhGFP4-hIgG1-Fc
Plasmid#105576PurposeT7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusionDepositorInsertHuman IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
UseIn vitro transcriptionAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4581
Plasmid#171124PurposeA kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.DepositorInsert3_sAID
UseOtherMutationnon applicableAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FDIO-ArchT-GFP
Plasmid#124640PurposeAAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoterDepositorInsertArchT
UseAAV; Flp/frtTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-PPP2CB-C-HA
Plasmid#195179PurposeVector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Blast DEST JNKKTRmRuby2
Plasmid#59154PurposeLentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human)
UseLentiviralTagsmRuby2ExpressionMammalianPromoterPGKAvailable SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHTC-HaloTag_RAB7A:1-207
Plasmid#211096PurposeMammalian expression of full-length human RAB7A. C-terminal HaloTag.DepositorInsertRAB7A:M1-C207 (RAB7A Human)
TagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits