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179,521 results

Showing: 11561 - 11600 of 179521 results

  2. pTA-Mob 2.0

    Plasmid
    #149662
    Purpose
    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.
    Depositor
    Bogumil Karas
    Publication
    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:
    Type
    Empty backbone
    Use
    Synthetic Biology; Conjugation helper plasmid
    Expression
    Bacterial
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    149662-plasmid-map-sequence-id-379005

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  6. pSLQ10875

    Plasmid
    #183963
    Purpose
    CBA-hyperdCas12a-HA-KRAB-mCherry
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, KRAB, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CBA
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183963-plasmid-map-sequence-id-363118

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  8. pAAV.hSyn.iGluSnFR3.v857.PDGFR

    Plasmid
    #178329
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857
    Depositor
    Kaspar Podgorski
    Publication
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has service
    AAV1
    Insert
    pAAV hSyn iGluSnFR3 v857.PDGFR
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and PDGFR TM Domain
    Expression
    Mammalian
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178329-plasmid-map-sequence-id-361540

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  10. pH6-MBP-TEV-BirA

    Plasmid
    #179694
    Purpose
    Expresses His6 tagged MBP-BirA fusion protein
    Depositor
    Mark Arbing
    Insert
    BirA
    Tags
    His6-MBP
    Expression
    Bacterial
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179694-plasmid-map-sequence-id-352195

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  16. pOpen-GBDpol

    Plasmid
    #165501
    Purpose
    Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable DNA Polymerase
    Use
    Synthetic Biology
    Available since
    July 26, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165501-plasmid-map-sequence-id-338429

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  19. pRecT_2

    Plasmid
    #167546
    Purpose
    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
    Depositor
    Howard Hang
    Publication
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Insert
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    Expression
    Bacterial
    Promoter
    IPTG
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167546-plasmid-map-sequence-id-332204

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  23. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166700-plasmid-map-sequence-id-329224

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  29. pM1s3AsG

    Plasmid
    #137921
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Publication
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    J23101
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    137921-plasmid-map-sequence-id-322876

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  31. JD639

    Plasmid
    #160398
    Purpose
    Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformation
    Depositor
    Jorge Dubcovsky
    Publication
    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    35S::vitis miR396-resistant GRF4-GIF1 chimera
    Use
    Binary vector
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    160398-plasmid-map-sequence-id-318342

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  32. pUltra-Plus-H2B-mTagBFP2

    Plasmid
    #161790
    Purpose
    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Bicistronic
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC and UbC promoter
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161790-plasmid-map-sequence-id-316820

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  34. C1-MPAct-mCherry

    Plasmid
    #155222
    Purpose
    Monitor changes in the density of membrane-proximal F-actin (MPA)
    Depositor
    Tobias Meyer
    Publication
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
    Tags
    mCherry
    Expression
    Mammalian
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155222-plasmid-map-sequence-id-310131

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  36. pLEX307-APN-puro

    Plasmid
    #158454
    Purpose
    Constitutive lentiviral expression of human APN.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    APN (ANPEP Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158454-plasmid-map-sequence-id-308145

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  37. AIMTOR A757

    Plasmid
    #140829
    Purpose
    AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757
    Depositor
    Anne Bonnieu
    Publication
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AIMTOR A757 (ULK1 Human)
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140829-plasmid-map-sequence-id-305143

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  39. P307-FBP_6

    Plasmid
    #139697
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway
    Depositor
    Daniel Voytas
    Publication
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    Use
    Luciferase
    Expression
    Plant
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139697-plasmid-map-sequence-id-296513

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  40. pCAGGs-Venus

    Plasmid
    #127346
    Purpose
    Expresses Venus constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus Fluorescent Protein
    Expression
    Mammalian
    Promoter
    CAGGS Promoter
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127346-plasmid-map-sequence-id-246571

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Showing: 11561 - 11600 of 179521 results