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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,026 results for: Sis

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Showing: 11561 - 11600 of 24026 results

  1. pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

    Plasmid
    #136646
    Purpose
    Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC
    Depositor
    Maria Balda, Karl Matter
    Article
    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.
    Insert
    SH3BP1R312A (RNAi resistant) (SH3BP1 Human)
    Tags
    HA (MQDLPGNDNSTAG)
    Expression
    Mammalian
    Mutation
    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTā€¦
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136646-plasmid-map-sequence-id-271745

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  7. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

    Plasmid
    #115490
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ī©_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    115490-plasmid-map-sequence-id-238520

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  23. pLsCas13aGG

    Plasmid
    #131012
    Purpose
    Backbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of LsCas13a
    GFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131012-plasmid-map-sequence-id-258107

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  34. pCI-TPI_WT-xrRNA-4H

    Plasmid
    #108368
    Purpose
    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108368-plasmid-map-sequence-id-209437

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  36. cycLuc_SuMMVs

    Plasmid
    #119210
    Purpose
    Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with SuMMVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119210-plasmid-map-sequence-id-232040

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Showing: 11561 - 11600 of 24026 results