20,128 results

Showing: 8791 - 8820 of 20128 results

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ACTN2-pEGFP

Plasmid

#52669

Purpose

Overexpression of GFP-tagged α-actinin-2

Depositor

Johannes Hell

Article

Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

Insert

actinin 2 (ACTN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1ab-Prkd1

Plasmid

#139314

Purpose

Diacylglycerol biosensor

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)

Expression

Mammalian

Mutation

T208A, N313D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-Alpha-5-Integrin-12

Plasmid

#54970

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Alpha-5 Integrin (ITGA5 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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2C-EGFP

Plasmid

#69071

Purpose

Expresses EGFP under MERVL_LTR

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

EGFP

Expression

Mammalian

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR5'_ubi:loxP-EGFP-loxP

Plasmid

#27322

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:loxP-EGFP-loxP

Use

Multisite gateway 5' entry vector

Available Since

April 5, 2011

Availability

Academic Institutions and Nonprofits only

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pErCas12a EZ Clone

Plasmid

#132641

Purpose

Expresses ErCas12a and an sgRNA to target a region of interest

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

AAV and CRISPR

Tags

3x HA and SV40 NLS

Expression

Mammalian

Mutation

Q926R (Please see depositor comments) and BsaI si…

Promoter

CMV Promoter

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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hyeA3A-BE4max

Plasmid

#157944

Purpose

Mammalian expression of hyeA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

hyeA3A-BE4max

Use

CRISPR

Expression

Mammalian

Mutation

N57G

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-IPP1-His

Plasmid

#118978

Purpose

Bacterial expression plasmid for Inorganic pyrophosphatase

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

inorganic diphosphatase IPP1 (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTetON-DeAct-GS1

Plasmid

#89454

Purpose

Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-GS1 (GSN Human)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning cytoplasmic Gsn amino acids 5…

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBad-HPII

Plasmid

#105839

Purpose

expresses E. coli HPII catalase with N-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

hydroperoxidase II catalase

Tags

6x his tag

Expression

Bacterial

Promoter

pBad/arabinose

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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v1em-Nterm-PE2max-U6-sgRNA

Plasmid

#198732

Purpose

AAV genome encoding N-terminal PE2max and U6 expression cassette

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

Nterm PE2max-NpuN

Use

AAV

Promoter

EFS

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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HA-Rab7A

Plasmid

#131417

Purpose

Expresses HA-tagged human Rab7A in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

human Rab7A

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMAZ-SK

Plasmid

#73962

Purpose

self-killing gRNA plasmid pMAZ-SK

Depositor

Alex Nielsen

Article

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Insert

gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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7TC

Plasmid

#24315

Depositor

Roel Nusse

Article

ten Berge et al Cell Stem Cell. 2008 Nov 6. 3(5):508-18.

Insert

7xTcf-mCherry

Use

Lentiviral

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pMi(3xP3-EGFP)

Plasmid

#102540

Purpose

Transgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP marker

Depositor

Michalis Averof, Charalambos Savakis

Article

Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

Insert

Minos transpoable element with 3xP3-EGFP marker

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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p426MET25_sfpHluorin (MRV55)

Plasmid

#115697

Purpose

superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)

Depositor

Eckhard Boles

Article

Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

Insert

sfpHluorin

Expression

Yeast

Mutation

for introduced mutations see publication

Promoter

MET25

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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PKC delta WT

Plasmid

#16386

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta isoform 2 (Prkcd Mouse)

Tags

HA

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

Plasmid

#197044

Purpose

In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

Depositor

Liqun Luo

Article

Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

Inserts

pCAG-FSF-LSL
SP-HA-HRPtm
WPRE-bGHpolyA

Tags

HA, myc

Expression

Mammalian

Promoter

pCAG

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Zeo-Ezh2

Plasmid

#40794

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Ezh2 (Ezh2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-TRAF6-wt

Plasmid

#21624

Depositor

John Kyriakis

Article

Zhong et al Mol Cell Biol. 2004 Oct . 24(20):9165-75

Insert

TNF receptor-associated factor 6 (Traf6 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-hTERT-HA

Plasmid

#1772

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Tags

2XHA

Expression

Mammalian

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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LIC1B_Caspase-LOVN7C-7

Plasmid

#104629

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation

Depositor

Jim Wells

Article

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Tags

His and LOV2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRK6-HA-TAK1

Plasmid

#44160

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only
DsRed2-N1

Plasmid

#54493

Purpose

Localization: N1 Cloning Vector, Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Tags

DsRed2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA4TO-mito-mCherry-10xGCN4_v4

Plasmid

#60914

Purpose

A fusion protein of the SunTag10xGCN4 v4 peptide array to a mitochondrial targeting domain and mCherry

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

mito-mCherry

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-ZapCY1

Plasmid

#36319

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ZapCY1 high affinity Zn(II) sensor

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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pTKLP-tetA

Plasmid

#71325

Purpose

tetA landing pad template plasmid

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCMMP-MCS-IRES-eGFP-P2A-iCas9

Plasmid

#178533

Purpose

For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

Depositor

Ryoji Yao

Article

Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

Insert

iCaspase9 (CASP9 Human)

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-dCas9-BFP

Plasmid

#46910

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, and HA

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCL12-sfGFP

Plasmid

#98961

Purpose

Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCL12 (CXCL12 Human)

Tags

Superfolder GFP

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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20,128 results