We narrowed to 22,836 results for: Sis

1. pY71-tet.I.12

   Plasmid

   #133533

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 15 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY71-tet.I.11

   Plasmid

   #133532

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY71-tet.I.10

   Plasmid

   #133531

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pY71-tet.I.9

   Plasmid

   #133530

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pY71-tet.I.7

   Plasmid

   #133529

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pY71-tet.I.6

   Plasmid

   #133528

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pY71-tet.I.5

   Plasmid

   #133527

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pY71-tet.I.3

   Plasmid

   #133525

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pY71-tet.I.2

   Plasmid

   #133524

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pY71-tet.I.4

    Plasmid

    #133526

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pY71-tet.I.1

    Plasmid

    #133523

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Ad-MKP-3 C293S

    Plasmid

    #131525

    Purpose

    Expresses MKP-3 with C293S mutation in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Mutation

    changed Cysteine 293 to Serine

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ad-FOXO1 1-558

    Plasmid

    #131531

    Purpose

    Expresses FOXO1 1-558 in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

    Insert

    Forkhead box O1 (Foxo1 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Mutation

    only reserved amino acid 1-558

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ad-FOXO1 1-456

    Plasmid

    #131532

    Purpose

    Expresses FOXO1 1-456 in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

    Insert

    Forkhead box O1 (Foxo1 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Mutation

    only reserved amino acid 1-456

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ad-FOXO1 1-280

    Plasmid

    #131534

    Purpose

    Expresses FOXO1 1-280 in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

    Insert

    Forkhead box O1 (Foxo1 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Mutation

    only reserved amino acid 1-280

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAcGP67B human Fzd4 CRD (38–160)

    Plasmid

    #132621

    Purpose

    expresses human Fzd4 CRD (38-160) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Shen et al Cell Res. 2015 Sep;25(9):1078-81. doi: 1

    Insert

    Fzd4 CRD (38–160) (FZD4 Human)
    

    Expression

    Insect

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZ_P-ICL1-eGFP

    Plasmid

    #126716

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    ICL1

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZ_P-ACS1-eGFP

    Plasmid

    #126715

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Expression

    Yeast

    Promoter

    ACS1

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZ_P-ALD-eGFP

    Plasmid

    #126714

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Expression

    Yeast

    Promoter

    ALD

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZ_P-HXT1-eGFP

    Plasmid

    #126719

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Expression

    Yeast

    Promoter

    HXT1

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZ_P-ADH3-eGFP

    Plasmid

    #126713

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Expression

    Yeast

    Promoter

    ADH3

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJL1-neoR

    Plasmid

    #117048

    Purpose

    In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    neoR
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEG302 22aa SunTag VP64 gRNA17 (FWA)

    Plasmid

    #120250

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZA-YFP-R5

    Plasmid

    #106257

    Purpose

    Expresses Venus YFP fused with silaffin-R5 silica affinity peptide under the control of TetO

    Depositor

    Urartu Seker

    Article

    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

    Insert

    Yellow fluorescent protein
    

    Tags

    Added silaffin R5 peptide to C terminus of YFP af…

    Expression

    Bacterial

    Promoter

    pL-TetO

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-myc-Sema4A RNAiR

    Plasmid

    #115172

    Purpose

    expresses RNAi-resistant Sema4A with N-terminal myc tag

    Depositor

    Suzanne Paradis

    Article

    McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

    Insert

    Sema4A (Sema4a Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Silent mutation between amino acids in ORF (401-4…

    Promoter

    CMV

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUC 3GLA

    Plasmid

    #71762

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBAD-MTX-u36_Snifit

    Plasmid

    #119045

    Purpose

    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

    Insert

    MTX-u36_Snifit
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    36TAG R44L H45Q

    Promoter

    pBAD

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD-MTX-u51_Snifit

    Plasmid

    #119046

    Purpose

    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

    Insert

    MTX-u51_Snifit
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    51TAG R44L H45Q

    Promoter

    pBAD

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBAD-MTX-u23_Snifit

    Plasmid

    #119044

    Purpose

    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

    Insert

    MTX-u23_Snifit
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    23TAG R44L H45Q

    Promoter

    pBAD

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCarCBADE

    Plasmid

    #117757

    Purpose

    Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised CarCBA, Protein CarD, Ferredoxin CarE
    

    Expression

    Bacterial

    Promoter

    PLac(UV5)

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRPL28 SpHIS5 (pNTI663)

    Plasmid

    #115433

    Purpose

    Expresses wild-type RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Expression

    Yeast

    Mutation

    MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRPL28(Q38P) SpHIS5 (pNTI664)

    Plasmid

    #115434

    Purpose

    Expresses Q38P RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Expression

    Yeast

    Mutation

    Q38P; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRPL28(H39L) SpHIS5 (pNTI665)

    Plasmid

    #115435

    Purpose

    Expresses H39L RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Expression

    Yeast

    Mutation

    H39L; MfeI site in intron

    Promoter

    ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHES 946

    Plasmid

    #112644

    Purpose

    blue light inducible cryptochrome based optogenetic expression system

    Depositor

    Hana El-Samad

    Article

    Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

    Insert

    RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Budding Yeast, Mustard Weed, Synthetic)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. C2 png genomic in Casper

    Plasmid

    #112971

    Purpose

    P element vector for transposon insertion of png genomic DNA into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Fenger et al Development. 2000 Nov;127(22):4763-74.

    Insert

    png genomic DNA (png Fly)
    

    Use

    P insertion vector

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. C2 M2 png-myc in Casper4

    Plasmid

    #112968

    Purpose

    P element vector for transposon insertion of Myc-tagged png into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Fenger et al Development. 2000 Nov;127(22):4763-74.

    Insert

    png-myc (png Fly)
    

    Use

    P insertion vector

    Tags

    myc epitope

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Hsh155 T178A R181A R182A R186A (N4A)

    Plasmid

    #111940

    Purpose

    This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A R181A R182A R186A mutation

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

    Insert

    Hsh155 T178A R181A R182A R186A (N4A)
    

    Expression

    Bacterial and Yeast

    Mutation

    T178A R181A R182A R186A

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Hsh155 Q773A R775A R776A Q778A (C4A)

    Plasmid

    #111946

    Purpose

    This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutation

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

    Insert

    Hsh155 Q773A R775A R776A Q778A (C4A)
    

    Expression

    Bacterial and Yeast

    Mutation

    Q773A R775A R776A Q778A

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET27b-ecDHFR-N23PP

    Plasmid

    #110828

    Purpose

    Bacterial expression of E.coli DHFR N23PP mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBV163

    Plasmid

    #108364

    Purpose

    Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.

    Depositor

    Scott Moye-Rowley

    Article

    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

    Insert

    Knock-in Cg TDH3 promoter in Cg YAP1 gene
    

    Use

    Replace c.glabrata yap1 promoter with c. glabrata…

    Promoter

    TDH3

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge