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171,784 results

Showing: 8881 - 8910 of 171784 results

  1. pCAG-GluA2-Fab-HC-Halo

    Plasmid
    #230061
    Purpose
    Expresses heavy chain Fab fragment of anti-GluA2 fused to HaloTag. Can be used with light chain to produce Fab in HEK cells.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 Fab heavy chain fragment
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230061-plasmid-map-sequence-id-460113

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  3. pJC2528 - CLYBL-Neo-CAGGS-LSL-dCas9-TagBFP-KRAB-CLYBL (LSL-CRISPRi)

    Plasmid
    #250253
    Purpose
    Homology donor for knock-in of CRISPRi system into CLYBL locus with addgene plasmids 62196 and 62197. Lox-Stop-Lox requires cre delivery for activation.
    Depositor
    Jacob Corn
    Article
    Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:
    Insert
    dCas9-mTagBFP-KRAB
    Use
    CRISPR
    Tags
    HA-2xSV40 NLS-mTagBFP-KRAB
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Jan. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    250253-plasmid-map-sequence-id-498726

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  5. pPIDNB2

    Plasmid
    #169902
    Purpose
    Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mNeonGreen is fused to H2B to allow for visualization of the nucleus.
    Depositor
    Richard Schneider
    Article
    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05
    Type
    Empty backbone
    Use
    Avian expression
    Expression
    Mammalian
    Promoter
    Dox inducible
    Available Since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    169902-plasmid-map-sequence-id-334686

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  8. pET-Impact-T4gp41

    Plasmid
    #228207
    Purpose
    Expression in BL21(DE3) of T4 helicase
    Depositor
    Stephen Benkovic
    Insert
    41 (41 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228207-plasmid-map-sequence-id-453945

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  9. pET-Impact-T4gp59

    Plasmid
    #228206
    Purpose
    Expression in BL21(DE3) of WT T4 helicase loader
    Depositor
    Stephen Benkovic
    Insert
    59 (59 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228206-plasmid-map-sequence-id-453944

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  11. GRM4-DuET

    Plasmid
    #213302
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM4 (GRM4 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213302-plasmid-map-sequence-id-431810

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  26. pFsynW SYB2 reporter

    Plasmid
    #197276
    Purpose
    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYB2 and HaloTag (Vamp2 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    N/A
    Promoter
    hSyn (human synapsin I promoter)
    Available Since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197276-plasmid-map-sequence-id-389092

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  30. pvPE-V4

    Plasmid
    #240288
    Purpose
    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Article
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Insert
    pvPE-V4
    Use
    CRISPR
    Tags
    SV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240288-plasmid-map-sequence-id-488805

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Showing: 8881 - 8910 of 171784 results