165,947 results

1. scFv-sfGFP-DNMT3A1

   Plasmid

   #102278

   Purpose

   The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

   Depositor

   Margaret Goodell

   Article

   Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

   Insert

   DNMT3A1 (DNMT3A Human)
   

   Use

   Lentiviral

   Tags

   scFV-sfGFP

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-HA-HA-humanCMYC

   Plasmid

   #74164

   Purpose

   Mammalian expression of human cMyc

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   cMyc (MYC Human)
   

   Tags

   2x HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-mCherry-CAAX

   Plasmid

   #129285

   Purpose

   Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

   Depositor

   Carlos Carmona-Fontaine

   Article

   Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

   Insert

   Fluorescent reporter mCherry fused to CAAX polybasic sequence
   

   Use

   Lentiviral

   Tags

   CAAX

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. STING-V1

   Plasmid

   #124262

   Purpose

   C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

   Depositor

   Eric Schirmer

   Insert

   TMEM173 (STING1 Human)
   

   Tags

   Venus1

   Expression

   Mammalian

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

   Plasmid

   #125713

   Purpose

   Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Has Service

   AAV1 and AAV5

   Insert

   eOPN3
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 331-429

   Promoter

   hSyn1

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

   Plasmid

   #44028

   Depositor

   Celeste Simon

   Article

   Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

   Insert

   mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P402A, P577A, N813A

   Promoter

   CMV

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA

   Plasmid

   #218527

   Purpose

   sgRNA targeting human EIF2AK2/PKR

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK2 (EIF2AK2 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-Puro_hCOL4A2-Myc-His-WPRE

   Plasmid

   #198080

   Purpose

   Express hCOL4A2 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A2 (COL4A2 Human)
   

   Tags

   TEV-Myc-His

   Expression

   Mammalian

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CA

   Plasmid

   #20960

   Purpose

   piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   Gateway Destination Vector
   

   Use

   piggybac

   Expression

   Mammalian

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.3/EV

    Plasmid

    #120848

    Purpose

    Control vector, 20nt random sequence between gateway recombination sites

    Depositor

    Jan Rehwinkel

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. G555

    Plasmid

    #216483

    Purpose

    Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

    Depositor

    Christophe Danelon

    Insert

    plsB, plsC, cdsA, pssA
    

    Use

    Cell-free expression

    Promoter

    T7 for each gene

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. His-GFP-Rab27a

    Plasmid

    #170861

    Purpose

    Mammalian expression of His-GFP-Rab27a.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Rab-27a (RAB27A Human)
    

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-KG-GST-YAP

    Plasmid

    #33052

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP1 (YAP1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH2.2_mCherry-MS2x12

    Plasmid

    #205521

    Purpose

    Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS316-RGR-GFP

    Plasmid

    #51056

    Purpose

    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. PEmaxNG-_2star_IVT

    Plasmid

    #227678

    Purpose

    plasmid for SpCas9-NG-PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    NG-PEmax_2_star
    

    Use

    CRISPR

    Tags

    no

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mscv2.2-NAIP5

    Plasmid

    #60205

    Purpose

    Mouse NAIP5 (B6 allele), expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of NAIP5

    Depositor

    Russell Vance

    Article

    Tenthorey et al Mol Cell. 2014 Apr 10;54(1):17-29. doi:

    Insert

    NAIP5
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-HyPer7-MEM

    Plasmid

    #136465

    Purpose

    Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    Farnselation tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHACK-GAL4>QF2

    Plasmid

    #80275

    Purpose

    GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Expression

    Insect

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEXSY-hyg2.1_LtIFT43_Flag

    Plasmid

    #194433

    Purpose

    Expression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolae

    Depositor

    Alan Brown

    Article

    Meleppattu et al Cell. 2022 Dec 22;185(26):4986-4998.e12.

    Insert

    Intraflagellar transport complex subunit 43
    

    Use

    Leishmania tarentolae expression

    Tags

    3X Flag and Linker

    Promoter

    L.donovani promoter elements

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. FLX1.8NFATGFPpd2HS4

    Plasmid

    #169096

    Purpose

    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

    Depositor

    Geoffrey Owens

    Inserts

    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    

    Use

    Lentiviral

    Promoter

    NFAT enhancer + synthetic TATA box

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. VAMP2-pHuji

    Plasmid

    #105289

    Purpose

    VAMP2 tagged with pH-sensitive red fluorescent protein pHuji

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    pHuji

    Expression

    Mammalian

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. GHRHR-Tango

    Plasmid

    #66292

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GHRHR (GHRHR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

24. Raf-1 GST RBD 1-149

    Plasmid

    #13338

    Depositor

    Channing Der

    Article

    Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-1

    Insert

    Raf-1 (RAF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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25. Flag-hPIASy

    Plasmid

    #15208

    Depositor

    Ke Shuai

    Article

    Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

    Insert

    PIASy (PIAS4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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26. pKDsgRNA-FRT

    Plasmid

    #113398

    Purpose

    expresses gRNA for Cas9 FRT targetting

    Depositor

    Jan Michiels

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    exo, beta, gam, sgRNA-FRT
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV_BiPVe4_GCaMP6f

    Plasmid

    #213939

    Purpose

    AAV construct expressing GCaMP6f driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_GCaMP6f

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB and AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA4-TO-Puromycin-mVenus-MAP

    Plasmid

    #44118

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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29. pT3-EF1aH c-Met

    Plasmid

    #86498

    Purpose

    Mammalian expression of human c-Met

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. HypoxCR

    Plasmid

    #59946

    Purpose

    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

    Depositor

    Anne Le

    Article

    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

    Inserts

    GFP-Pest
    mCherry-Geminin
    

    Use

    Lentiviral

    Tags

    FLAG and PEST

    Expression

    Mammalian

    Promoter

    CMV and min CMV

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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