159,557 results

1. tetO-FOSL1

   Plasmid

   #170687

   Purpose

   doxycycline-inducible overexpression of FOSL1

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   FOSL1 (FOSL1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL33

   Plasmid

   #227430

   Purpose

   Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

   Depositor

   Pierre Santucci

   Insert

   mWasabi
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL34

   Plasmid

   #227431

   Purpose

   Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

   Depositor

   Pierre Santucci

   Insert

   tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-MRLC1 T18D, S19D

   Plasmid

   #35682

   Depositor

   Tom Egelhoff

   Article

   Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T18D and S19D

   Promoter

   CMV

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

5. TFORF0799

   Plasmid

   #141705

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ATF3 (ATF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG36

   Plasmid

   #35126

   Depositor

   John McCusker

   Article

   Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

   Insert

   nat1
   

   Use

   Yeast replicating

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS418

   Plasmid

   #11256

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. NylCK_pET28a

   Plasmid

   #215422

   Purpose

   Expression construct for NylCK gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCK
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-Ets2

   Plasmid

   #28128

   Depositor

   Pumin Zhang

   Article

   Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

   Insert

   Ets2 (ETS2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2705

    Plasmid

    #142126

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ESRRB (ESRRB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mScarlet-I-LaminB

    Plasmid

    #98831

    Purpose

    In vivo visualization of the nuclear envelope (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    CytERM
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT004

    Plasmid

    #64759

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, V5 to an HA-6xHIS epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRE-Medium-FT

    Plasmid

    #31914

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    PTight Tet-responsive PminCMVd

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBIS

    Plasmid

    #17817

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIRESneo-FLAG/HA Ago2 corrected

    Plasmid

    #10822

    Depositor

    Thomas Tuschl

    Article

    Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    3 silent mutations in orf

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-E12-vioABECD

    Plasmid

    #66537

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-pur-CAG-mCherry

    Plasmid

    #159458

    Purpose

    AAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection gene

    Depositor

    Yuejun Chen

    Article

    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT001

    Plasmid

    #64758

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a V5 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTagRFP657-C1

    Plasmid

    #31872

    Purpose

    For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

    Depositor

    Vladislav Verkhusha

    Article

    Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

    Insert

    TagRFP657
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTW073

    Plasmid

    #64754

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a V5 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. HS-aPKC-CAAX-mKate2

    Plasmid

    #105950

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    aPKC-CAAX
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET30ax

    Plasmid

    #85761

    Purpose

    A bacterial expression vector with a His-tag at N and C-terminus.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Type

    Empty backbone

    Use

    Tags

    His tag (N-term and C-term)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJUMP23-1A(sfGFP)

    Plasmid

    #126970

    Purpose

    Level 1 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

    Plasmid

    #188767

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. LAMP1-mRFP-FLAG

    Plasmid

    #34611

    Purpose

    3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

    Depositor

    David Sabatini

    Article

    Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    2xFlag and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CD44S pBabe-puro

    Plasmid

    #19127

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    CD44 standard (CD44 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Use

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWZL Hygro-NMYC TRF2 deltaB

    Plasmid

    #16349

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF2 (TERF2 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deleted Basic Domain (aa1-44)

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pISH-Cux1

    Plasmid

    #74341

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Cux1. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    cut-like homeobox 1 (Cux1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge