We narrowed to 148,387 results for: ins;

1. pCytERM-mRhubarb720-N1

   Plasmid

   #197191

   Purpose

   Expresses the fusion protein of CytERM-mRhubarb720 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   CytERM-mRhubarb720
   

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMHE-NLS-mEGFP

   Plasmid

   #105527

   Purpose

   T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Nucleoplasmin
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Mutation

   NLS of nucleoplasmin (encoding amino acids KRPAAT…

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDA303

   Plasmid

   #195199

   Purpose

   Overexpressing 6His-TetR in E. coli.

   Depositor

   Rahul Sarpeshkar

   Article

   Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

   Insert

   TetR
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T5 promoter

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLSSmKate1-N1

   Plasmid

   #31866

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   LSS-mKate1
   

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Promoter

   CMV

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-RSV-GCaMP6s

   Plasmid

   #80146

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Darrell Kotton

   Article

   Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Promoter

   RSV

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 hE47

   Plasmid

   #16059

   Depositor

   Robert Benezra

   Article

   Jen et al Genes Dev. 1992 Aug . 6(8):1466-79.

   Insert

   E47 (TCF3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein

   Plasmid

   #208378

   Purpose

   Episomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split intein

   Depositor

   Daniel Hawiger

   Article

   Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   human CMV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

   Plasmid

   #208379

   Purpose

   Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

   Depositor

   Daniel Hawiger

   Article

   Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   human CMV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PT7lacO-RF1-His6

   Plasmid

   #224372

   Purpose

   Express RF1 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   RF1 (prfA E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIEx4-Cre

    Plasmid

    #198280

    Purpose

    Expresses Cre in insect cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pIEx4-Cre
    

    Expression

    Insect

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. UbC-(mNeonGreen)4-tDeg

    Plasmid

    #129404

    Purpose

    (mNeonGreen)4-tDeg fluorogenic protein

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    (mNeonGreen)4-tDeg
    

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMO9075

    Plasmid

    #117481

    Purpose

    empty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecR

    Depositor

    Judy Wall

    Article

    Keller et al Methods Enzymol. 2011;497:503-17. doi: 1

    Insert

    Aph(3')-IIa promoter
    

    Expression

    Bacterial

    Promoter

    Aph(3')-IIa promoter

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-I3-01-Myc

    Plasmid

    #80038

    Purpose

    Expression of I3-01

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    I3-01-Myc
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSP64T Activin betaB (DM#108)

    Plasmid

    #15045

    Depositor

    Douglas Melton

    Article

    Thomsen et al Cell. 1990 Nov 2. 63(3):485-93.

    Insert

    Activin Beta b (inhbb Frog)
    

    Expression

    Bacterial

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL11015

    Plasmid

    #117499

    Purpose

    FAST-Red selectable marker Golden Gate Level 1 Position 1

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
    

    Tags

    RFP

    Expression

    Plant

    Promoter

    AtOLE1

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL0_13 [pIP501 ORI]

    Plasmid

    #198927

    Purpose

    Level 0 part

    Depositor

    Nili Ostrov, Charlie Gilbert, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pIP501 ORI
    

    Use

    Synthetic Biology

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-2(KW)2-eGFP

    Plasmid

    #112736

    Purpose

    Express 2(KW)2-eGFP in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

    Insert

    2(KW)2-eGFP
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas1+2

    Plasmid

    #72676

    Purpose

    Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

    Depositor

    Udi Qimron

    Article

    Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

    Insert

    Cas1, Cas2
    

    Expression

    Bacterial

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_psychLight1

    Plasmid

    #198739

    Purpose

    Expresses psychLight1 in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

    Insert

    psychLight1
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-Neo HA-hSki

    Plasmid

    #10910

    Depositor

    Bob Weinberg

    Article

    Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    Ski (SKI Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGL0_3 [RSF1010 ORI]

    Plasmid

    #198920

    Purpose

    Level 0 part

    Depositor

    Nili Ostrov, Charlie Gilbert, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    RSF1010 ORI
    

    Use

    Synthetic Biology

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBAD-HisD-miRFP713-HO1

    Plasmid

    #197255

    Purpose

    Expresses the protein of miRFP713 and HO1 in bacteria

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP713
    

    Expression

    Bacterial

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. DS-SPcas

    Plasmid

    #48645

    Purpose

    Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Inserts

    Cas9
    tracrRNA precursor
    

    Use

    CRISPR

    Promoter

    proC and tracrRNA promoter

    Available Since

    Dec. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. pENTR-LUC (w158-1)

    Plasmid

    #17473

    Purpose

    Gateway entry vector, Firefly Luciferase

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Entry vector

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-IMX-DogTag

    Plasmid

    #113765

    Purpose

    Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

    Depositor

    Mark Howarth

    Article

    Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

    Insert

    IMX-DogTag
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLSSmKate1-C1

    Plasmid

    #31868

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSS-mKate1
    

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    CMV

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Dendra2-Farnesyl-5

    Plasmid

    #57717

    Purpose

    Localization: Membrane, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

28. LZRS-GADD45B

    Plasmid

    #24955

    Depositor

    Paul Khavari

    Article

    Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

    Insert

    GADD45B (GADD45B Human)
    

    Use

    Retroviral

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pccGFPG83I

    Plasmid

    #73650

    Purpose

    Fluorescent reporter for transmembrane protein dimerization negative control.

    Depositor

    Alessandro Senes

    Article

    Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

    Insert

    glycophorin A- G83I
    

    Expression

    Bacterial

    Mutation

    glycine 83 to isoleucine

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA TfR1-miniR

    Plasmid

    #181986

    Purpose

    MiniR genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniE labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    TfR1-MiniR
    

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pH2B-LSSmKate2-N1

    Plasmid

    #31903

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    H2B-LSSmKate2
    

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Promoter

    CMV

    Available Since

    Sept. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pH2B-miRFP720-N1

    Plasmid

    #197231

    Purpose

    Expresses the fusion protein of H2B-miRFP720 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    H2B-miRFP720
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTK21

    Plasmid

    #37396

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    Ran (RAN Human)
    

    Tags

    S-tag, TEV, and mCherry

    Expression

    Mammalian

    Mutation

    T24N

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)

    Plasmid

    #211861

    Purpose

    Nuclear-localized E3-DART ligase in domestication vector.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    3xNLS-HA

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEB1-mCerulean

    Plasmid

    #103968

    Purpose

    Plasmid encoding mCerulean

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCerulean
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    ProC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMTB

    Plasmid

    #112225

    Purpose

    Expression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.

    Depositor

    Sean Megason

    Article

    Wagner et al Science. 2018 Apr 26. pii: science.aar43

    Insert

    Super-folder GFP
    

    Use

    Zebrafish expression

    Promoter

    beta actin 2 (zebrafish)

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SRa-HA-SUMO2 (Sentrin 3)

    Plasmid

    #17360

    Purpose

    Mammalian expression of human SUMO2 with HA tag

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

    Insert

    SUMO2 (SUMO2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBS_36x-601_MA1+MA2+MA3

    Plasmid

    #114363

    Purpose

    36x Widom 601 nucleosome sequence

    Depositor

    Scot Wolfe

    Article

    Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

    Insert

    36X601-MA1_2_3
    

    Use

    Unspecified

    Expression

    Mammalian

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA GST PreScission p70 S6K1

    Plasmid

    #15511

    Depositor

    David Sabatini

    Article

    Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

    Insert

    p70 S6K1 (Rps6kb1 Rat)
    

    Tags

    GST and HA

    Expression

    Mammalian

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

40. PT7lacO-His6-IF2

    Plasmid

    #224367

    Purpose

    Express IF2 protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    IF2 (infB E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge