Skip to main content
Addgene
Showing: 101 - 150 of 1418 results

  1. p3E-DrCavin1a

    Plasmid
    #194291
    Purpose
    Multisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXM
    Depositor
    Rob Parton
    Article
    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.
    Insert
    cavin1a (cavin1a Zebrafish)
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    194291-plasmid-map-sequence-id-380041

    Enlarge

  2. InterCatch-2xL

    Plasmid
    #186908
    Purpose
    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)2 linker and the other side expressing EGFP and puromycin resistance gene
    Depositor
    Taekjip Ha, Allen Liu
    Article
    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100
    Insert
    TfR-(GGGGS)2-SpyCatcher003-sfCherry1-10
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    186908-plasmid-map-sequence-id-369405

    Enlarge

  4. pBRCA2.1.0-gDNA

    Plasmid
    #132448
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    BRCA2 (BRCA2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132448-plasmid-map-sequence-id-266805

    Enlarge

  7. pcDNA3-Lyso-TORCAR

    Plasmid
    #64929
    Purpose
    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.
    Depositor
    Jin Zhang
    Article
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Insert
    LAMP1-TORCAR (LAMP1 Human, Synthetic)
    Use
    Tags
    Cerulean, Full-length LAMP1, and YPet
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64929-plasmid-map-sequence-id-109691

    Enlarge

  21. WT Smarca4-V5 IRES-puro

    Plasmid
    #107058
    Purpose
    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107058-plasmid-map-sequence-id-201960

    Enlarge

  22. 6xHis-MBP-huErCas12a

    Plasmid
    #174685
    Purpose
    Expresses 6xHis-MBP tagged ErCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huErCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis
    Expression
    Bacterial
    Mutation
    Q926R mutation is observed as indicated from the …
    Promoter
    T7
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    174685-plasmid-map-sequence-id-346198

    Enlarge

  23. K785R Smarca4-sfGFP

    Plasmid
    #107057
    Purpose
    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    K785R
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107057-plasmid-map-sequence-id-201959

    Enlarge

  28. pcDNA3.1/HisA/Serca1

    Plasmid
    #74402
    Purpose
    expression of SERCA in mammalian cells
    Depositor
    Robert Nakamoto
    Insert
    SERCA1 (ATP2A1 rabbit)
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    NcoI site destroyed (silent) T898
    Promoter
    CMV
    Available since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. RCAS(A) mFgfr1 DN (CT#984)

    Plasmid
    #14006
    Depositor
    Cliff Tabin
    Insert
    Fgfr1 (Fgfr1 Mouse)
    Use
    Retroviral; Avian expression
    Tags
    Expression
    Mutation
    Dominant negative
    Promoter
    Available since
    Feb. 16, 2007
    Availability
    Academic Institutions and Nonprofits only
    14006-plasmid-map-sequence-id-465063

    Enlarge

  37. RCAS(A) mLef1 (CT#1141)

    Plasmid
    #14021
    Depositor
    Cliff Tabin
    Insert
    Lef1 (Lef1 Mouse)
    Use
    Retroviral; Avian expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2007
    Availability
    Academic Institutions and Nonprofits only

  38. RCAS(B) hSmo (CT#495)

    Plasmid
    #14016
    Depositor
    Cliff Tabin
    Insert
    Smoothened (SMO Human)
    Use
    Retroviral; Avian expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 15, 2007
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 150 of 1418 results