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Plasmid#196627PurposeScrambled control 1 for STING knock-out in murine cells.DepositorInsertmSTING scrambled gRNA 1
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti_SaCRISPR_GFP
Plasmid#118636PurposeTo clone sgRNA for SaCas9 (SauCas9)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterU6Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2Cre
Plasmid#82415PurposeLentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning siteDepositorInsertCre Recombinase
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationMutated BsmBI cut sitePromoterEFS (P2A)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2R
Plasmid#167932PurposelentiCRISPRv2 variant with mScarlet-I fluorescence marker.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.tRFP
Plasmid#57826PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_mCherry
Plasmid#229078PurposeSmall lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cellsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterEF1sAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
epiCRISPR
Plasmid#135960PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_Puro_sgAAVS1
Plasmid#187818PurposepLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1DepositorInsertsgAAVS1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_sgCtrl#1
Plasmid#174148PurposeLentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor siteDepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi
Plasmid#199808Purposevector for constitutive CRISPRi-mediated gene knockdown in S. aureusDepositorInsertsdCas9
sgRNA with GFP cassette
UseCRISPRTagsExpressionMutationPromoterP23 and PRAB17 (sgRNA) and SarAP1 (GFP)Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniCRISPRoff-v1
Plasmid#197030PurposeVector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized miniCRISRPoff-v1
UseTagsExpressionMammalianMutationD52R / T132R / D228A / D406APromoterCBh, CMV, hU6Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15a_Kan_MLS_Rosa26_Short_CRISPRDel
Plasmid#97008PurposeRosa26 locus targeting vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ2072.2_CRISPRlator
Plasmid#140674PurposeCRISPRlatorDepositorInsertCRISPRlator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-TP
Plasmid#203589PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_zeo_sgTP53
Plasmid#183180PurposepLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53DepositorInsertsgTP53 (TP53 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_PROX1_1+3
Plasmid#121176PurposeLentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.DepositorInsertCas9-P2A-puro and U6 promoters driven expression of gRNAs
UseCRISPR and LentiviralTagsP2A-puroExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_sgCtrl#2
Plasmid#174149PurposeLentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor siteDepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1_SDHB
Plasmid#177981Purposelentiviral vector expressing Cas9 and a sgRNA targeting SDHBDepositorInsertsgRNA targeting SDHB (SDHB Human)
UseLentiviralTagsExpressionMammalianMutationN/APromoterU6Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_MFE1
Plasmid#84612PurposeIntroduces DSB at MFE1 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_zeo_sgNT135
Plasmid#183181PurposepLentiCRISPR that is selectable with zeocin with a non-targeting sgRNADepositorInsertNT135
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only