We narrowed to 8,677 results for: sgRNA

1. ATP1A1_G8_Dual_sgRNA

   Plasmid

   #178104

   Purpose

   Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 sgRNA + user-specified sgRNA
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. px458_2A_GFP_sgRNA_LTN1

   Plasmid

   #127125

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA LTN1 (LTN1 Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCgsgRNA_crtYf

   Plasmid

   #153338

   Purpose

   Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

   Insert

   crRNA targeting crtYf
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. NmCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122091

   Purpose

   U6 driven NmCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnsgRNA

   Plasmid

   #172717

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   J23119-gRNA
   

   Expression

   Bacterial

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6a:sgRNA#1

   Plasmid

   #64245

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6b:sgRNA#3

   Plasmid

   #64247

   Purpose

   expresses sgRNA under U6b promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6c:sgRNA#4

   Plasmid

   #64248

   Purpose

   expresses sgRNA under U6c promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6a:sgRNA#2

    Plasmid

    #64246

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6d:sgRNA#5

    Plasmid

    #64249

    Purpose

    expresses sgRNA under U6d promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA.SFFV.tRFP

    Plasmid

    #169941

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgRNA_expression_vector

    Plasmid

    #210212

    Purpose

    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

    Depositor

    Albert Jeltsch

    Article

    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

    Type

    Empty backbone

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKDsgRNA-NT

    Plasmid

    #89960

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    empty gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LCV2_LacZ_sgRNA_2

    Plasmid

    #155093

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    LacZ_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. esgRNA_NbPDS

    Plasmid

    #231147

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting NbPDS

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting NbPDS
    

    Expression

    Plant

    Promoter

    PEBV sub-genomic

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459_MCM2_sgRNA

    Plasmid

    #232730

    Purpose

    Generates MCM2 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    MCM2 (MCM2 Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459_CDC45_sgRNA

    Plasmid

    #232732

    Purpose

    Generates CDC45 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    CDC45 (CDC45 Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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