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We narrowed to 3,043 results for: beta-actin

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Showing: 1501 - 1550 of 3043 results

  1. A5TSR

    Plasmid
    #50358
    Purpose
    expresses T.gondii MIC2(67-593) domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-A5TSR
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CHICK beta-actin promoter
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV.CAG.FLEX.iGluSnFR3.v857.GPI

    Plasmid
    #196218
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV CAG.FLEX iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196218-plasmid-map-sequence-id-405482

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  14. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid
    #199252
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity eSpCas9(1.1)-D10A nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A K1003A R1060A
    Promoter
    CAG promoter
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199252-plasmid-map-sequence-id-391383

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  16. pAK_DR30_CasRx_Puro

    Plasmid
    #134845
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    134845-plasmid-map-sequence-id-266706

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  17. AAV-CAG-flex-lck-smV5-4x6T

    Plasmid
    #196419
    Purpose
    Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smV5
    Use
    AAV and Cre/Lox; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    196419-plasmid-map-sequence-id-385571

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  23. pCAG-IRES2-p75NTR-GFP

    Plasmid
    #182947
    Purpose
    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal
    Depositor
    Pantelis Tsoulfas
    Insert
    p75NTR-mGFP
    Tags
    HA tag and mGFP
    Expression
    Mammalian
    Mutation
    The leader sequence is from preprotrypin
    Promoter
    CAG
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182947-plasmid-map-sequence-id-360205

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  27. PB-PE SpRY

    Plasmid
    #179082
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…
    Promoter
    CAG
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179082-plasmid-map-sequence-id-352656

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  29. pCAT007

    Plasmid
    #171634
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171634-plasmid-map-sequence-id-336493

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  30. PA-Dre

    Plasmid
    #169706
    Purpose
    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells
    Depositor
    Dali Li
    Article
    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11
    Insert
    Dre246-nMag-T2A-pMag-Dre247
    Use
    AAV
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CAG
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    169706-plasmid-map-sequence-id-333284

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  32. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid
    #140083
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP
    Depositor
    Keith Joung
    Insert
    Human codon optimized pCAG BE4max with P2A-EGFP
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A (SpCas9)
    Promoter
    CAG
    Available Since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    140083-plasmid-map-sequence-id-292705

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  41. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid
    #102888
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102888-plasmid-map-sequence-id-262466

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  48. pAAV.CAG.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106203
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    CAG
    Available Since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106203-plasmid-map-sequence-id-201030

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  49. pCAGGS-hPhyB621-mCherry-HRasCT

    Plasmid
    #100282
    Purpose
    Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    Phytochrome B (1-621 aa) (PHYB Mustard Weed)
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-621 aa)
    Promoter
    CAG promoter
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100282-plasmid-map-sequence-id-197258

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  50. pPBC-LG6f

    Plasmid
    #62808
    Purpose
    pPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6f-ITR
    Expression
    Bacterial and Mammalian
    Promoter
    CAG
    Available Since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    62808-plasmid-map-sequence-id-123121

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Showing: 1501 - 1550 of 3043 results