159,238 results

1. pENTR4-myc-nuc (w556-1)

   Plasmid

   #20026

   Purpose

   Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Gateway entry vector

   Tags

   Myc and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_KRAS_p.G12V

   Plasmid

   #81665

   Purpose

   Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   G12V

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1162_pAAV-HLP-EmGFP-SpA

   Plasmid

   #109313

   Purpose

   Plasmid for AAV EmGFP Mammalian Expression

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   EmGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EMTB-mScarletI

   Plasmid

   #137801

   Purpose

   Visualization of microtubules

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   Ensconsin microtubule binding domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. IRE1 alpha KA-pcDNA3.EGFP

   Plasmid

   #13010

   Depositor

   Fumihiko Urano

   Article

   Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

   Insert

   Inositol Requiring Enzyme-1 (ERN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R4L, K599A

   Promoter

   CMV

   Available since

   Nov. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-Flex-TC66T

   Plasmid

   #48331

   Purpose

   Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA

   Depositor

   Liqun Luo

   Article

   Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

   Insert

   TVA receptor mCherry fusion with the 66T mutation
   

   Use

   AAV and Cre/Lox

   Tags

   mCherry fusion to TVA receptor

   Expression

   Mammalian

   Mutation

   66T

   Promoter

   CAG

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIP

   Plasmid

   #79009

   Purpose

   For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGs

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-21(+)Tm9D8* TrpB

   Plasmid

   #207517

   Purpose

   Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

   Depositor

   Thomas Huber

   Article

   Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

   Insert

   Tryptophan synthase β-subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. BirA-Myc_N_MYOD1

   Plasmid

   #167716

   Purpose

   Expresses MYOD1 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   MYOD1 (MYOD1 Human)
   

   Use

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBpaRS3tRNA

    Plasmid

    #197102

    Purpose

    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

    Insert

    TyrRS variant, amber suppressor tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYZ038

    Plasmid

    #200227

    Purpose

    universal template to build tRNA-gRNA array for multiplex editing CREEPY

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    tRNA-gRNA scaffold
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cyclin E-pBI-EGFP

    Plasmid

    #16654

    Depositor

    Bert Vogelstein

    Article

    Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

    Insert

    Cyclin E (CCNE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204723

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJMP1183

    Plasmid

    #119254

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV_TRET_hNgn1_UBC_Bla

    Plasmid

    #61473

    Purpose

    3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection marker

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn1 (NEUROG1 Human)
    Bla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. SiRES COR1C-Halo

    Plasmid

    #195153

    Purpose

    image COR1C

    Depositor

    Gia Voeltz

    Article

    Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    CORO1C (CORO1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cav-GA

    Plasmid

    #209880

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    CAVIN1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHJ320

    Plasmid

    #20671

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Moesin (MSN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT/TO/FLAG/HA-HDLBP

    Plasmid

    #154450

    Purpose

    Expression of FLAG/HA-tagged HDLBP

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    HDLBP (HDLBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSF-G1(7AW)RS

    Plasmid

    #207516

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mCherry-Vav2

    Plasmid

    #129623

    Purpose

    RhoGEF

    Depositor

    Dorus Gadella

    Article

    Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

    Insert

    Vav2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-flag-YTHDF2-N

    Plasmid

    #52302

    Purpose

    Flag-tagged YTHDF2 N-terminal domain, made by mutating to E384(GAA) to stop codon(TAA)

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Changed E384 to stop codon

    Promoter

    T7

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

23. CAG-Flex-RG

    Plasmid

    #48333

    Purpose

    Cre-dependant (flex) rabies glycoprotein

    Depositor

    Liqun Luo

    Article

    Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

    Insert

    Rabies Glycoprotein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDestTol2-ubi-ASAP1

    Plasmid

    #121987

    Purpose

    ubiqutously express ASAP1 voltage biosensor

    Depositor

    GuangJun Zhang

    Article

    Silic et al J Vis Exp. 2018 Apr 25;(134). doi: 10.37

    Insert

    ASAP1
    

    Use

    Tol2 transposon destination vector

    Tags

    Expression

    Mutation

    Promoter

    zebrafish ubiquitin promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pJMP2844

    Plasmid

    #160675

    Purpose

    Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBa.NgCAM-mCherry

    Plasmid

    #180248

    Purpose

    Mammalian expression of NgCAM

    Depositor

    Marvin Bentley

    Article

    Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

    Insert

    NgCAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Gal:mCherry-uTEV3

    Plasmid

    #135453

    Purpose

    Yeast expression of uTEV3 fused to mCherry

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry-uTEv3
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    S219V mutation improves stability , I138T/S153N/T…

    Promoter

    Gal

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AiP14253 - pAAV-AiE0380h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4253)

    Plasmid

    #208165

    Purpose

    AiE0380h_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Mich et al bioRxiv 2023.09.20.558718

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. CL7-LbCas12a

    Plasmid

    #164658

    Purpose

    Bacterial expression of LbCas12a using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHyVec11

    Plasmid

    #34794

    Depositor

    Rob Steele

    Type

    Empty backbone

    Use

    Hydra expression

    Tags

    Intergenic region between Hydra replication facto…

    Expression

    Mutation

    Promoter

    Hydra vulgaris actin promoter

    Available since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge