165,775 results

1. Twin-Strep(R)-Tev-GSGS-Ubiquitin

   Plasmid

   #186803

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   Twin-Strep(R)-Tev-GSGS-Ubiquitin
   

   Tags

   GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

   Expression

   Bacterial

   Mutation

   Strep tag Lysine's mutated to Arginine

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

   Plasmid

   #221359

   Purpose

   Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. EGFP

   Plasmid

   #176015

   Purpose

   Mammalian expression of EGFP. Parton lab clone DFS

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   EGFP
   

   Expression

   Mammalian

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa

   Plasmid

   #221360

   Purpose

   Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa; to be used with Anti-V5 [IPI-SV5-Pk1] heavy chain (Plasmid 221359) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAAV MP-84 mRFP

   Plasmid

   #206926

   Purpose

   The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

   Depositor

   Markus Grompe

   Article

   Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

   Insert

   mRFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   MP-84

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

   Plasmid

   #176851

   Purpose

   lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   hYAP1-5SA (YAP1 Human)
   

   Use

   Lentiviral

   Mutation

   S61A, S109A, S127A, S164A, S381A (phosphorylation…

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 hygro

   Plasmid

   #24150

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

   Depositor

   Bob Weinberg

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA

   Available Since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. HiBiT-FZD7

   Plasmid

   #195845

   Purpose

   Expresses human FZD7 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD7 (FZD7 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pREDawn-AmpR-MCS

   Plasmid

   #188978

   Purpose

   For red light-induced bacterial expression of gene of interest (AmpR)

   Depositor

   Heikki Takala

   Article

   Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. HyPer7.2DAAO-NLS

    Plasmid

    #168302

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-EGFP-C1-HsNXF-1_K

    Plasmid

    #146740

    Purpose

    Mammalian Expression of HsNxf-1

    Depositor

    Elisa Izaurralde

    Article

    Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

    Insert

    HsNxf-1 (NXF1 Human)
    

    Expression

    Mammalian

    Mutation

    S144G relative to NM_006362.4

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ptfLC3

    Plasmid

    #21074

    Purpose

    Mammalian expression of rat LC3 fused to mRFP and EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJC5-Tmem192-3xHA

    Plasmid

    #102930

    Purpose

    Lentiviral expression of a lysosomal tag (Tmem192-HA)

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    Tmem192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. paavCAG-iCre

    Plasmid

    #51904

    Purpose

    For AAV expression of iCre recombinase

    Depositor

    Jinhyun Kim

    Article

    Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

    Insert

    improved Cre recombinase
    

    Use

    AAV and Cre/Lox

    Promoter

    CAG

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ153

    Plasmid

    #69304

    Purpose

    Gateway entry vector with pco-dCas9-3X(SRDX)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-3X(SRDX) (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Mutation

    SRDX (X3) repressor fusion; NLS fusion

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMF230

    Plasmid

    #62546

    Purpose

    Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

    Depositor

    Michael Franklin

    Article

    Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

    Insert

    GFP mut2
    

    Use

    Broad host range plasmid

    Promoter

    trc

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRZeroT

    Plasmid

    #120276

    Purpose

    Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRT029

    Plasmid

    #127969

    Purpose

    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

    Plasmid

    #110854

    Purpose

    Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    sgRNA scaffold with spacer
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    U6

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

    Plasmid

    #67974

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDestattB (pCM268)

    Plasmid

    #68313

    Purpose

    Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Type

    Empty backbone

    Use

    Zebrafish transgenesis

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. ABE-NW1

    Plasmid

    #240615

    Purpose

    Adenine base editor with narrowed editing window

    Depositor

    Tingting Jiang

    Article

    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

    Insert

    TadA8e(N108Q,M151C,Q154Y)-linker-nCas9 (D10A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    TadA8e(N108Q,M151C,Q154Y),Cas9 (D10A)

    Available Since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLX307 P2A GFP Luciferase

    Plasmid

    #193674

    Purpose

    Constitutive lentiviral expression of Luciferase

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. eGFP-KIF5B-Rigor

    Plasmid

    #172204

    Purpose

    Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF5B (Kif5b Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. FUW-M2rtTA

    Plasmid

    #20342

    Purpose

    Lentiviral plasmid expressing the reverse tetracycline transactivator

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

    Insert

    M2rtTA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. pmVen-RPA70-IRES-RPA32-IRES-RPA14

    Plasmid

    #211431

    Purpose

    mammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14

    Depositor

    Helmut Pospiech

    Insert

    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    

    Tags

    mVenus (on RPA70)

    Expression

    Mammalian

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAVS1-P-CAG-mCh

    Plasmid

    #80492

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    mCherry
    

    Use

    Donor vector (human)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. ppyCAG_RNaseH1_WKKD

    Plasmid

    #111905

    Purpose

    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

    Available Since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCherry.90 alpha

    Plasmid

    #108222

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 alpha

    Depositor

    Didier Picard

    Article

    Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

    Insert

    mCherry-Hsp90a (HSP90AA1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. HA-14-3-3 zeta

    Plasmid

    #116888

    Purpose

    Expresses HA-tagged human 14-3-3 zeta in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Article

    Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

    Insert

    14-3-3 zeta (YWHAZ Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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