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We narrowed to 13,064 results for: LIC

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Showing: 1601 - 1650 of 13064 results

  2. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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  3. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

    Plasmid
    #193769
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1ΔR198A/R210A (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193769-plasmid-map-sequence-id-380211

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  5. pCW-ND-Cdt1-mCherry-Puro

    Plasmid
    #193766
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-mCherry (CDT1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193766-plasmid-map-sequence-id-380221

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  9. pCW-ND-Cdt1-HA-Puro

    Plasmid
    #193767
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-HA (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193767-plasmid-map-sequence-id-380216

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  13. An R57A, R59A CAAX pMT2

    Plasmid
    #206119
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206119-plasmid-map-sequence-id-415935

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  14. An R57A, R59A HA CAAX pMT2

    Plasmid
    #206126
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Oct. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    206126-plasmid-map-sequence-id-412911

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  21. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Article
    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Promoter
    U6
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232438-plasmid-map-sequence-id-462755

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  23. pCW-ND-Cdt1Δ499-546-HA-Puro

    Plasmid
    #193768
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1Δ499-546 (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193768-plasmid-map-sequence-id-380214

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  26. pCW57-mCherry-2A-BRD4 Iso AdelCTD

    Plasmid
    #137722
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)
    Depositor
    Scott Floyd
    Article
    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi
    Insert
    BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Truncated at amino acid 1328
    Promoter
    Tight TRE promoter
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    137722-plasmid-map-sequence-id-352678

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  27. pCW57-mCherry-2A-BRD4 Iso CdelET

    Plasmid
    #137723
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)
    Depositor
    Scott Floyd
    Article
    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi
    Insert
    BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Deleted amino acids 600-682
    Promoter
    Tight TRE promoter
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    137723-plasmid-map-sequence-id-288120

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  33. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  34. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available Since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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Showing: 1601 - 1650 of 13064 results