Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,274 results for: pcDNA3.1+

Showing: 1601 - 1650 of 5274 results

  1. pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C)-HA

    Plasmid
    #239767
    Purpose
    Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060
    Insert
    Scn8a (Scn8a Mouse)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    K1546TAG, Y371C
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239767-plasmid-map-sequence-id-477107

    Enlarge

  6. pcDNA3.1-IARS2-H112A/P115A

    Plasmid
    #232338
    Purpose
    Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Putative isoleucine binding deficiency mutation H…
    Promoter
    CMV
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    232338-plasmid-map-sequence-id-474537

    Enlarge

  7. pcDNA3.1-IARS2-K664A/K667A

    Plasmid
    #232339
    Purpose
    Expresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Putative tRNA binding deficiency mutation K664A A…
    Promoter
    CMV
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    232339-plasmid-map-sequence-id-474533

    Enlarge

  9. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid
    #191212
    Purpose
    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay
    Depositor
    Mohan Babu
    Insert
    SARS-CoV-2 spike_WT (S SARS CoV-2)
    Use
    Tags
    HiBiT (high affinity small nanoluciferase fragmen…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191212-plasmid-map-sequence-id-437009

    Enlarge

  10. pcDNA3.1-DDR1-F866Y-V5/HIS

    Plasmid
    #236009
    Purpose
    expression of the F866Y kinase defective mutant variant of human DDR1 that might be associated with lung Adenocarcinoma
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    human DDR1-F866Y receptor tyrosine kinase, full length (DDR1 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    F866Y substitution
    Promoter
    CMV
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236009-plasmid-map-sequence-id-469590

    Enlarge

  11. pcDNA3.1-PDGFRA-V561D-V5/HIS

    Plasmid
    #234762
    Purpose
    Expression of the V561D mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor and GIST-Plus Syndrome, , constantly activated
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Oncotarget. 2017 Nov 27;8(65):109319-109
    Insert
    PDGFRA-V561D receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    V561D substitution
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234762-plasmid-map-sequence-id-468956

    Enlarge

  13. pcDNA3.1-PDGFRA-R487L-V5/HIS

    Plasmid
    #234761
    Purpose
    Expression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal Tumor
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    PDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    R487L substitution
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234761-plasmid-map-sequence-id-468953

    Enlarge

  14. pcDNA3.1-PDGFRA-D842V-V5/HIS

    Plasmid
    #234763
    Purpose
    Expression of the D842V mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor, constantly activated
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Oncotarget. 2017 Nov 27;8(65):109319-109
    Insert
    PDGFRA-D842V receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D842V substitution
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234763-plasmid-map-sequence-id-468952

    Enlarge

  16. pcDNA3.1-PDGFRA-T674I-V5/HIS

    Plasmid
    #234756
    Purpose
    Expression of the T674I mutant variant of human PDGFRA, which is associated with Idiopathic Hypereosinophilic Syndrome, resistant to imatinib
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    PDGFRA-T674I receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    T674I substitution
    Promoter
    CMV
    Available since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    234756-plasmid-map-sequence-id-468865

    Enlarge

  17. YFP Rab11 Q70L in pcDNA3.1

    Plasmid
    #90467
    Purpose
    RhoA mediated cell migration
    Depositor
    Narasimhan Gautam
    Insert
    Rab11 Q70L
    Use
    Tags
    mEYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    90467-plasmid-map-sequence-id-197844

    Enlarge

  44. pcDNA3.1-AntiHer2-LC122-TAG

    Plasmid
    #226839
    Purpose
    Contains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporation
    Depositor
    Abhishek Chatterjee
    Article
    Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46
    Inserts
    anti-HER2 light chain
    anti-HER2 heavy chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    amino acid 122 of light chain mutated to TAG
    Promoter
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    226839-plasmid-map-sequence-id-451751

    Enlarge

  45. pcDNA3.1-AntiHer2-HC121-TAG

    Plasmid
    #226836
    Purpose
    Contains trastuzumab with a TAG codon at position 121 of the heavy chain for ncAA incorporation
    Depositor
    Abhishek Chatterjee
    Article
    Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46
    Inserts
    anti-HER2 light chain
    anti-HER2 heavy chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    amino acid 121 of heavy chain mutated to TAG
    Promoter
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    226836-plasmid-map-sequence-id-451750

    Enlarge

  46. pcDNA3.1-AntiHer2-HC197-TAG

    Plasmid
    #226838
    Purpose
    Contains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation
    Depositor
    Abhishek Chatterjee
    Article
    Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46
    Inserts
    anti-HER2 light chain
    anti-HER2 heavy chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    amino acid 197 of heavy chain mutated to TAG
    Promoter
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    226838-plasmid-map-sequence-id-451747

    Enlarge

  47. His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)

    Plasmid
    #187727
    Purpose
    Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells
    Depositor
    Sandra Gabelli
    Article
    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi
    Insert
    NEDD4L with residues 226-382 removed (NEDD4L Human)
    Use
    Tags
    His-HRV 3C site
    Expression
    Mammalian
    Mutation
    deletion of residues 226-382
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    187727-plasmid-map-sequence-id-372425

    Enlarge

  48. His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)

    Plasmid
    #187728
    Purpose
    Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells
    Depositor
    Sandra Gabelli
    Article
    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi
    Insert
    NEDD4L with residues 418-495 removed (NEDD4L Human)
    Use
    Tags
    His-HRV 3C site
    Expression
    Mammalian
    Mutation
    deletion of residues 418-495
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    187728-plasmid-map-sequence-id-372426

    Enlarge

Showing: 1601 - 1650 of 5274 results