We narrowed to 14,239 results for: TIM
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pMYO127TAG-SepT
Plasmid#34625DepositorInsertMyoglobin-127TAG
Tags6xHISExpressionBacterialMutationcodon-optimized + an amber stop codon was introdu…PromoterIppAvailable SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEBG-NHERF2
Plasmid#28292DepositorAvailable SinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMB66
Plasmid#47946PurposeExpresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormPromoterhsp-16.48Available SinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLN592 (FuGW-S(USF1)p-mKate2)
Plasmid#105204Purposesynthetic promoter USF1 drives mKate2 expressionDepositorInsertS(USF1)p-mKate2
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLN379 (FuGW-S(E2F1)p-mKate2)
Plasmid#105177Purposesynthetic promoter E2F1 drives mKate2 expressionDepositorInsertS(E2F1)p-mKate2
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
HSD17B4
Plasmid#39156PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-eSpCAS9-NG-pA
Plasmid#131466PurposeExpresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized eSpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBN449
Plasmid#129555PurposeGAL4-induced expression of codon-optimised FLP recombinase with unfused mNeongreen (mNG) as a marker in C. elegansDepositorInsert15xUAS-∆pes-10-Flp-SL2
TagsmNeonGreenExpressionWormPromoterUAS-∆pes-10Available SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP
Plasmid#84254PurposeExpresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent markerDepositorInsertSa sgCXCR4-1
UseCRISPR and LentiviralExpressionMammalianPromotermouse U6Available SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJY3919
Plasmid#113332PurposeHermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoterDepositorInsertsbeta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase
ExpressionYeastAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK_proB_eGFP
Plasmid#59845PurposeConstitutively expresses eGFP with proB promoterDepositorInsertEnhanced Green Fluorescent Protein
Tags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproBAvailable SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
130_pSKB3-LUMP-P.leioghnati
Plasmid#65894PurposeBacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetimeDepositorInsertLUMP
ExpressionBacterialAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSDMA65
Plasmid#128354PurposeCodon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.DepositorTypeEmpty backboneUseCRISPRPromoterACT1Available SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK1_eGFP
Plasmid#59853PurposeConstitutively expresses eGFP with proK1 promoterDepositorInsertEnhanced Green Fluorescent Protein
Tags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK1Available SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMB62
Plasmid#47944PurposeExpresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormPromotereef-1A.1 (eft-3)Available SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-T3-SpCAS9-NG-pA
Plasmid#131465PurposeExpresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized SpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-puro-a5 guide B
Plasmid#194011PurposeFor CRISPR knockout of integrin alpha5DepositorInsertsingle-guide RNA (a5_guide B)
UseCRISPRAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-puro-aV guide A
Plasmid#194008PurposeFor CRISPR knockout of integrin alphaVDepositorInsertsingle-guide RNA (aV_guide A)
UseCRISPRAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-puro-aV guide B
Plasmid#194009PurposeFor CRISPR knockout of integrin alphaVDepositorInsertsingle-guide RNA (aV_guide B)
UseCRISPRAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-puro-a5 guide A
Plasmid#194010PurposeFor CRISPR knockout of integrin alpha5DepositorInsertsingle-guide RNA (a5_guide A)
UseCRISPRAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ytdTomato-CaURA3
Plasmid#118461PurposeYeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection. *An updated version of this plasmid is available: #168054 pFA6a-link-ytdTomato-URA3.DepositorInsertytdTomato
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_10
Plasmid#60581Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_10
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGB-SpCas9
Plasmid#210533PurposeCloning A.thaliana codon optimized SpCas9 CDS as GreenGate module BDepositorInsertSpCas9
UseSynthetic BiologyAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ155
Plasmid#69306PurposeGateway entry vector with AteCas9D10ADepositorInsertAteCas9D10A (Arabidopsis codon optimized)
UseCRISPRMutationD10A mutation; NLS fusionAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3C-ARL16(CO)-His6
Plasmid#216485PurposeUsed for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.DepositorInsertADP-ribosylation factor-like 16 (ARL16 Human)
Tagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionBacterialPromoterT7Available SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DAT-myc
Plasmid#107457PurposeMammalian expression plasmid for codon optimized hDAT with internal MYC tag inserted in ECL2DepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PP255
Plasmid#78934PurposePromoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLN377 (FuGW-S(SP1)p-mKate2 )
Plasmid#105176Purposesynthetic promoter SP1 drives mKate2 expressionDepositorInsertS(SP1)p-mKate2
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only
