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We narrowed to 2,466 results for: crispr system

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Showing: 1651 - 1700 of 2466 results

  1. pYPQ-dzCas9-Act3.0

    Plasmid
    #158414
    Purpose
    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158414-plasmid-map-sequence-id-332091

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  2. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102886
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102886-plasmid-map-sequence-id-194712

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  3. pYPQ-dSpRY-Act3.0

    Plasmid
    #158416
    Purpose
    CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    158416-plasmid-map-sequence-id-337116

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  4. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102889
    Purpose
    PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102889-plasmid-map-sequence-id-198247

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  5. pCMV-eGFP-mPlum-HA

    Plasmid
    #200009
    Purpose
    Donor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.
    Depositor
    Thomas Noll
    Article
    Hertel et al Front Bioeng Biotechnol. 2022 Oct 13;10:
    Insert
    eGFP, mPlum
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200009-plasmid-map-sequence-id-398431

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  6. pYPQ-dpcoCas9-Act3.0

    Plasmid
    #158408
    Purpose
    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158408-plasmid-map-sequence-id-330919

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  15. T777T

    Plasmid
    #113082
    Purpose
    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    RNAi
    Expression
    Bacterial
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113082-plasmid-map-sequence-id-218958

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  31. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Promoter
    Tight TRE promoter
    Available Since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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  35. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  37. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  38. pYPQ-dzCas9-NG-Act3.0

    Plasmid
    #158415
    Purpose
    CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158415-plasmid-map-sequence-id-331006

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  39. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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  41. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid
    #187959
    Purpose
    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Inserts
    Blasticidin resistance
    FKBP12_F36V
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187959-plasmid-map-sequence-id-373708

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  42. pYPQ-dAaCas12b-Act3.0

    Plasmid
    #158413
    Purpose
    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158413-plasmid-map-sequence-id-337077

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  50. pXPR_068

    Plasmid
    #164555
    Purpose
    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral; Flp/frt
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164555-plasmid-map-sequence-id-330129

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Showing: 1651 - 1700 of 2466 results