We narrowed to 4,630 results for: lum

1. FEMP

   Plasmid

   #191973

   Purpose

   FRET-based indicator of ER-mitochondria proximity (FEMP)

   Depositor

   Luca Scorrano

   Article

   Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

   Insert

   AkapYFP-Tav2a-CFP-sac1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLentiCas9-T2A-BFP

   Plasmid

   #78547

   Purpose

   Cas9 protein with BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   In frame with Cas9

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-IRES-Hygro_RGECO1.2

   Plasmid

   #164592

   Purpose

   Stable integration and expression of RGECO1.2 in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   R-GECO1.2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

   Promoter

   CMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-X1-Neo-GFP-FAM134B-LIRmut

   Plasmid

   #109027

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   FAM134B-LIRmut (RETREG1 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   DDFELL to DDAEAL aa 453-458 to interrupt FAM134B …

   Promoter

   EIF-1a promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p0521s

   Plasmid

   #62862

   Purpose

   Episome vector for Phaeodactylum tricornutum (pCC1BAC-based)

   Depositor

   Hamilton Smith

   Article

   Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Episomal vector for phaeodacty…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HaloTag-sec61b

   Plasmid

   #186959

   Purpose

   Express sec61b connected with N-terminus HaloTag

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Sec61 (SEC61B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVX-puro_G-CEPIA1er

   Plasmid

   #164590

   Purpose

   Stable integration and expression of G-CEPIA1er in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   G-CEPIA1er
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

   Promoter

   CMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMC-6-GFP-P2A-NptII-9

   Plasmid

   #197741

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   EGFP-P2A-NptII bicistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEmerald-PDHA1-N-10

   Plasmid

   #54225

   Purpose

   Localization: Mitochondria, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   PDHA1 (PDHA1 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pEGFP-TYR

    Plasmid

    #32781

    Depositor

    Ruth Halaban

    Article

    Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NAX_CAGGS_CAS9_m4_VPR_EBFP2

    Plasmid

    #167881

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC19/ER-mChe

    Plasmid

    #183163

    Purpose

    Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    ER-mCherry
    

    Use

    Tags

    25 aa ER signal of Glycine soja kunitz-type tryps…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3-SREBP-2-GFP

    Plasmid

    #204202

    Purpose

    Mammalian expression of SREBP-2

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-2 (SREBF2 Human)
    

    Use

    Tags

    T7x3 and eGFP

    Expression

    Mammalian

    Mutation

    multiple silent mutations

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ptyrEGFP-TYR

    Plasmid

    #32782

    Depositor

    Ruth Halaban

    Article

    Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tyrosinase

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pMC-6-BeYDV-Rep-RepA-9

    Plasmid

    #197715

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV Rep/RepA proteins: native ORFs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RA-Sec61β

    Plasmid

    #153978

    Purpose

    Dimerization dependent fluorescent protein RA domain fused to ER protein Sec61β

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    Sec61 Transolocon Subunit Beta (SEC61B Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-X1-Neo-GFP-ATL3

    Plasmid

    #109024

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3 (ATL3 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti_CMV:Flag-GeNL

    Plasmid

    #208841

    Purpose

    Expresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    GeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmGFP-Sec16S

    Plasmid

    #15775

    Depositor

    Benjamin Glick

    Article

    Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

    Insert

    Sec16S (SEC16B Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NAX_CAGGS_CAS9_m4_KRAB_mcherry

    Plasmid

    #167878

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

21. BFP-Dcp1a

    Plasmid

    #153973

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Use

    Tags

    mTagBFP-c1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQCXIP GFP-MOSPD2 WT

    Plasmid

    #186467

    Purpose

    Expression of human MOSPD2 fused to EGFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-HiBiT-CD81

    Plasmid

    #162593

    Purpose

    N-terminally HiBiT-tagged human CD81 under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    human CD81 (CD81 Human, Synthetic, human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRZ-mOct4-CNL

    Plasmid

    #65718

    Purpose

    mOct4 reporter plasmid (expresses CNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    murine Oct4 promoter and CNL
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. lenti-ER-mTagBFP2

    Plasmid

    #216127

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-ProlactinEndoplasmicReticulumSignalSequence-mTagBFP2-KDEL-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRetroX-Tight-MCS_PGK-GpNLuc

    Plasmid

    #70185

    Purpose

    Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GB4542

    Plasmid

    #215261

    Purpose

    Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GB4401

    Plasmid

    #215252

    Purpose

    Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRetroX-Tight-MCS_PGK-OgNLuc

    Plasmid

    #70186

    Purpose

    Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. REEP5-mEmerald

    Plasmid

    #186964

    Purpose

    Express REEP5/DP1 with C-terminus mEmerald

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Reep5 (Reep5 Mouse)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG/ATP9A(E195Q)-HA

    Plasmid

    #209244

    Purpose

    Mammalian expression of ATP9A mutant E195Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP9A (ATP9A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E195Q

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRK5-Ufm1 ∆C3

    Plasmid

    #133866

    Purpose

    Expression of human UFM1 mutant lacking the last three C-terminal residues

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo)

    Plasmid

    #22131

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    synthethic oligonucleotide
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    synthethic oligonucleotide encoding shRNA targeti…

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GB-Dcp1b

    Plasmid

    #153979

    Purpose

    Dimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1b

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1b (DCP1B Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PERK 2: PERK.K618A.9E10.pCDNA

    Plasmid

    #21815

    Depositor

    David Ron

    Article

    Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

    Insert

    PERK (K618A) (Eif2ak3 Mouse)
    

    Use

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    kinase dead K618A mutant

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-HiBiT-CD63

    Plasmid

    #162591

    Purpose

    N-terminally HiBiT-tagged human CD63 under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    human CD63 (CD63 Human, Synthetic, human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRevTRE-hygro-GFP-Crumbs3a WT

    Plasmid

    #195217

    Purpose

    Retroviral Tet response vector containing GFP tagged Crumbs3a

    Depositor

    David Bryant, Ben Margolis

    Article

    Schluter et al Mol Biol Cell. 2009 Nov;20(22):4652-63.

    Insert

    CRB3A (CRB3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti-X1-Neo-GFP-ATL2

    Plasmid

    #109020

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2 (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SAC1delta452-587-FKBP-mCherry

    Plasmid

    #108131

    Purpose

    Recruitable inactive SAC1 (linker deletion)

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(1-451):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-451)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEGFP-N1 gp78fl

    Plasmid

    #37375

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    P179L and F262L

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV Ef1a DIO GeNL_SS hGtACR1

    Plasmid

    #233511

    Purpose

    Luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

    Inserts

    mNeonGreen
    ISN-IE
    hGtACR1
    

    Use

    AAV

    Tags

    Gaussia luciferase signal sequence

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. PM-ABKAR

    Plasmid

    #61506

    Purpose

    Expression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence

    Depositor

    Takanari Inoue, Jin Zhang

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Use

    Tags

    Lyn kinase sequence (see comments)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

44. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

    Plasmid

    #167902

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSALECTNK-TEM1(S70A,D179G)/csTEM1

    Plasmid

    #81163

    Purpose

    pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    TEM-1 beta-lactamase
    

    Use

    Tags

    Codon swapped TEM-1 from H26 to W290 and His6x

    Expression

    Bacterial

    Mutation

    Deleted the sec transport tag. Plasmid encodes H2…

    Promoter

    lac promoter

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Ch-gelsolN

    Plasmid

    #37262

    Depositor

    Ikuo Wada

    Article

    Nagaya et al J Cell Biol. 2008 Jan 14;180(1):129-43.

    Insert

    gelsolin (GSN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deleted amino acids 380-731

    Promoter

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

47. pMTH PKC alpha

    Plasmid

    #8409

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C alpha (Prkca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

48. human-GFAP-EGFP

    Plasmid

    #169473

    Purpose

    We generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    EGFP-hGFAP (GFAP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET28a Gx-mNG-NL

    Plasmid

    #169106

    Purpose

    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - mNeonGreen - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. NAX_CAGGS_CAS9_m4_VPR_KanR neo

    Plasmid

    #167885

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge