We narrowed to 22,696 results for: Sis;

1. pET24a HAB1 (172-511)

   Plasmid

   #177844

   Purpose

   Bacterial Expression of HAB1

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   Protein phosphatase 2C 16
   

   Use

   Tags

   6xHis-GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVG1

   Plasmid

   #111444

   Purpose

   Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgScADE2/stop-codon repair
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO GFP DNAJB8

   Plasmid

   #19504

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB8 (DNAJB8 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGE_En01

   Plasmid

   #71534

   Purpose

   Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi, Ikuko Hara-Nishimura

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. drebrin-His

   Plasmid

   #40363

   Depositor

   Phillip Gordon-Weeks

   Article

   Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

   Insert

   drebrin E2 (DBN1 Human)
   

   Use

   Tags

   His and T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCK081 desmaMCS:minpromCerulean,exorh:mCherry

   Plasmid

   #200014

   Purpose

   Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPP5

   Plasmid

   #209967

   Purpose

   Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P_sppQ)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDGE657

   Plasmid

   #153255

   Purpose

   M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRetroSuper-shFyn

   Plasmid

   #26985

   Depositor

   Joan Massague

   Article

   Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

   Insert

   shRNA Fyn (FYN Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pCDH-CAG-NCreIntN-T2A-mTagBFP2

    Plasmid

    #160500

    Purpose

    Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDUP1

    Plasmid

    #223516

    Purpose

    inactive cogfp under control of Ptet, cogfp under control of Ptac

    Depositor

    Yolanda Schaerli

    Article

    Ljiljana et al eLife13:RP97216

    Inserts

    CoGFP
    CoGFP inactivated
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    anti-dimerization mutations S129G, 22 C154S, D156…

    Promoter

    Ptac, Ptac and Ptet, and Ptet

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Luc-SIRT1 mutant 3'UTR

    Plasmid

    #20380

    Depositor

    Charles Lowenstein

    Article

    Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

    Promoter

    Available Since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCK074 p5E exorh:EGFP

    Plasmid

    #195951

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEG302 5aa SunTag VP64 nog

    Plasmid

    #115480

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTn7·X-nia

    Plasmid

    #122592

    Purpose

    Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS/Pm->Nia
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. YFP-Rac1(N17)

    Plasmid

    #11395

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 mutated to Asparagine.

    Promoter

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+PIF6CreN

    Plasmid

    #131780

    Purpose

    Mammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite system

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PIF6CreN
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-mTagBFP2-MRTF-B

    Plasmid

    #182062

    Purpose

    Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mTagBFP2-MRTF-B (Mrtfb Mouse)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA-3xFLAG-TCAB1

    Plasmid

    #51630

    Purpose

    Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

    Depositor

    Steven Artandi

    Article

    Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

    Insert

    TCAB1 (WRAP53 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pGEX-4T-1-RIPK3

    Plasmid

    #78827

    Purpose

    To purify GST-tagged recombinant RIPK3 protein

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CLPIT Cry2PHR-mCh-RhoA

    Plasmid

    #42959

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-Off promoter

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAL188

    Plasmid

    #22277

    Purpose

    Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    TiamDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLL3.7-K122-TEAD-p-H2B-mCherry

    Plasmid

    #139648

    Purpose

    Reporter assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    IRES-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-Talin-L432G

    Plasmid

    #26725

    Depositor

    Anna Huttenlocher

    Article

    Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

    Insert

    Talin (Tln1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Leucine 432 to Glycine to block ability o…

    Promoter

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pFB-GTE-COI1

    Plasmid

    #29516

    Depositor

    Ning Zheng

    Article

    Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

    Insert

    COI1 (COI1 Mustard Weed)
    

    Use

    Tags

    GST and TEV cleavage site

    Expression

    Insect

    Mutation

    Note that the first AA in TEV site is R, not E

    Promoter

    Available Since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pDONOR-tagBFP-PSM-dTOMATO

    Plasmid

    #100604

    Purpose

    Donor scafold with dTOMATO positive selection module, and tagBFP negative selection module

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    dTOMATO-T2A-Puro and tagBFP

    Expression

    Mutation

    Promoter

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. UKNP6.1.1

    Plasmid

    #98386

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

    Plasmid

    #194519

    Purpose

    Dual fluorescence reporter for studying protein degradation

    Depositor

    Tommer Ravid

    Article

    Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

    Insert

    TIF4631 leader sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHygromycin (GB0211)

    Plasmid

    #68204

    Purpose

    Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Hygromycin resistance gene coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGWB450

    Plasmid

    #74832

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge