4,168 results

1. pUC57-mitoLbNOX

   Plasmid

   #74448

   Purpose

   This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   mitoLbNOX
   

   Tags

   Flag tag with linker and mitochondrial targeting …

   Mutation

   Human codon optimized

   Available Since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe K-Ras 12V

   Plasmid

   #12544

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   K-Ras 12V (KRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   12V constituitively activated

   Available Since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CMV-GCaMP6f

   Plasmid

   #40755

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6f
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-D1ER

   Plasmid

   #36325

   Depositor

   Roger Tsien, Amy Palmer

   Article

   Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

   Insert

   D1ER second generation cameleon (calcium sensor) targeted to ER
   

   Tags

   KDEL

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIRV-AP-1-mCherry

   Plasmid

   #118095

   Purpose

   Fluorescent reporter for AP-1 activation

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   mCherry
   

   Use

   Retroviral

   Promoter

   minimal promoter with AP-1 responsive element

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO/cmycDrosha

   Plasmid

   #10828

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

   Insert

   Drosha (DROSHA Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-puro SV40 LT

   Plasmid

   #13970

   Depositor

   Thomas Roberts

   Article

   Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

   Insert

   SV40 LT (SV40gp6 )
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTiger

   Plasmid

   #1728

   Purpose

   This is a 3rd generation lentiviral transfer vector (highest titer) from Nolan lab's FELIX vector system

   Depositor

   Garry Nolan

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Aug. 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUW-Sox10

   Plasmid

   #36978

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox10 (SOX10 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-CAG-hChR2-H134R-tdTomato

    Plasmid

    #28017

    Purpose

    AAV-mediated expression of hChR2(H134R) for neuronal excitation.

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Has Service

    AAV Retrograde

    Insert

    humanized ChR2-H134R-tdTomato
    

    Use

    AAV; Adeno associate viral

    Tags

    tdTomato

    Mutation

    H134R

    Promoter

    CAG

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57-CMV-ssRFP-GFP-KDEL

    Plasmid

    #128257

    Purpose

    Fluorescence reporter to monitor ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    ssRFPGFPKDEL
    

    Use

    Lentiviral

    Tags

    RFPGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-AKAR4

    Plasmid

    #61619

    Purpose

    FRET-based biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    AKAR4
    

    Tags

    Cerulean and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-PDGFRA

    Plasmid

    #23892

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Gateway donor vector

    Mutation

    M260I

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. rtTA-N144

    Plasmid

    #66810

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    Reverse tetracycline-controlled transactivator
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human EF1a

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-EGFP-C1-HsRCK

    Plasmid

    #25033

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    Rck (DDX6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-miniTurbo

    Plasmid

    #124647

    Purpose

    Expresses Flag-miniTurbo fusion for proximity labeling

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLionII

    Plasmid

    #1730

    Purpose

    This is a 3rd generation lentiviral transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system

    Depositor

    Garry Nolan

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-MET

    Plasmid

    #23889

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MET (MET Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-mRFP1

    Plasmid

    #21315

    Purpose

    MoMLV-based Retroviral vector, expresses mRFP1

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    monomeric Red Fluorescence Protein 1
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMA3790

    Plasmid

    #70110

    Purpose

    mtDNA elimination/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    hUNG1 Y147A
    Neo
    

    Tags

    None, human OTC mitochondrial targeting sequence,…

    Expression

    Mammalian

    Mutation

    Y147A, aa's 1-77 removed

    Promoter

    CMV, EF1a, and SV40 and native

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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