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pCMV3TAG8li-hRNF160-3FLAG

Plasmid

#159138

Purpose

human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

listerin E3 ubiquitin protein ligase 1 (LTN1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-gp78-FLAG

Plasmid

#62370

Purpose

Express C-terminally FLAG tagged gp78

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

gp78 (AMFR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

*

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-mCherry

Plasmid

#29585

Depositor

Anna Huttenlocher

Article

Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

Insert

mpx-mCherry

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pCas9/VRQR-sgRNA (BbsI)

Plasmid

#129725

Purpose

Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Plasmid

#58442

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1

Plasmid

#58431

Purpose

Expresses LexA-ER-B42 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B42

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-AsCpf1-Blast

Plasmid

#84750

Purpose

Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

AsCpf1

Use

CRISPR and Lentiviral

Tags

NLS-3xHA (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTRETightBI-RY-0

Plasmid

#31463

Depositor

Phil Sharp

Article

Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

Insert

eYFP and mCherry

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2012

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

Plasmid

#65454

Purpose

Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

ChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mutation

H134R variant

Promoter

hSyn1

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-Y1699C

Plasmid

#25364

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

Tyrosine 1699 mutated to Cysteine

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pCAG-mir30

Plasmid

#14758

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

mir30 cassette

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pBABE-hLin28B

Plasmid

#26358

Depositor

George Daley

Article

Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

Insert

lin-28 homolog B (LIN28B Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pGW1-mCherry-EGFP-PIM

Plasmid

#111759

Purpose

High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

Depositor

Lukas Kapitein

Article

Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

Insert

2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP

Use

Tags

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

CMV

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 WT

Plasmid

#69824

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-Rac1-Q61L

Plasmid

#12979

Depositor

Gary Bokoch

Insert

Rac1 constitutively active (RAC1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Q61L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-NKX3.1

Plasmid

#42897

Depositor

Edward Gelmann

Article

Steadman et al Nucleic Acids Res. 2000 Jun 15;28(12):23

Insert

NKX3.1 (NKX3-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T1-Smurf1 wt

Plasmid

#13502

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pPRIME-TET-GFP-recipient

Plasmid

#11655

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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Friend MLV Env YFP

Plasmid

#1820

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Envelope (env MuLV)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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