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Showing: 1751 - 1800 of 2321 results

  1. pYPQAT49

    Plasmid
    #223421
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for monocot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-LbCas12a-RRV-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223421-plasmid-map-sequence-id-454169

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  2. pYPQAT50

    Plasmid
    #223422
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for monocot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA was driven by separate ZmUbi1 promoter; Hygromycin for plants select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-LbCas12a-RRV-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223422-plasmid-map-sequence-id-453572

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  3. pYPQAT51

    Plasmid
    #223423
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223423-plasmid-map-sequence-id-453500

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  5. pHPHS800

    Plasmid
    #228240
    Purpose
    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Insert
    Cas9
    Use
    Tags
    Hygromycin phosphotransferase
    Expression
    Mammalian
    Mutation
    Promoter
    none
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228240-plasmid-map-sequence-id-454866

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  6. pET28-H12TEV-hEIF5B-587~1220

    Plasmid
    #208637
    Purpose
    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208637-plasmid-map-sequence-id-433646

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  8. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid
    #208626
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 191~307, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208626-plasmid-map-sequence-id-415912

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  9. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid
    #208627
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 299~398, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208627-plasmid-map-sequence-id-415915

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  10. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid
    #208629
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 212~285, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208629-plasmid-map-sequence-id-415924

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  16. KBB106

    Plasmid
    #185065
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain
    Depositor
    Ken Belanger
    Article
    Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Nup1 C-terminal region, truncated so C-terminal h…
    Promoter
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    185065-plasmid-map-sequence-id-367180

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  17. pTS2341_Tier3(SB)-Puro

    Plasmid
    #169639
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169639-plasmid-map-sequence-id-366789

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  18. pTS2351_Tier3(SB)-Zeo

    Plasmid
    #169648
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven ZeoR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169648-plasmid-map-sequence-id-366198

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  19. CD44I-c011

    Plasmid
    #175449
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175449-plasmid-map-sequence-id-353150

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  22. pLY133

    Plasmid
    #130961
    Purpose
    Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB4
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Anderson promoter J23100
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    130961-plasmid-map-sequence-id-266878

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  23. pLY86

    Plasmid
    #130949
    Purpose
    Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEA2-WTmis2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plux2
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    130949-plasmid-map-sequence-id-258930

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  24. pLY132

    Plasmid
    #130960
    Purpose
    Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB3
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130960-plasmid-map-sequence-id-257712

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  25. pLY130

    Plasmid
    #130952
    Purpose
    Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130952-plasmid-map-sequence-id-257710

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  30. pAC-LYC

    Plasmid
    #53270
    Purpose
    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53270-plasmid-map-sequence-id-82990

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  33. PHP.AX

    Plasmid
    #195218
    Purpose
    AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)
    Depositor
    Viviana Gradinaru
    Article
    Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103
    Insert
    Synthetic construct isolate AAV-PHP.AX VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    p41
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195218-plasmid-map-sequence-id-385556

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  36. pDM069

    Plasmid
    #216821
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold2
    Use
    Tags
    RIPGLIN and VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216821-plasmid-map-sequence-id-430130

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  37. pTS2340_Tier3(SB)-iRFP_Puro

    Plasmid
    #169638
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven iRFP670 and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169638-plasmid-map-sequence-id-364985

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  41. pR26 CAG/GFP Dest

    Plasmid
    #74281
    Purpose
    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning
    Depositor
    Ralf Kuehn
    Article
    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi
    Insert
    Rosa26 5-homology region
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    74281-plasmid-map-sequence-id-419887

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  43. pR26 CAG/GFP Asc

    Plasmid
    #74285
    Purpose
    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI
    Depositor
    Ralf Kuehn
    Article
    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi
    Insert
    Rosa26 5-homology region
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    74285-plasmid-map-sequence-id-357226

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  47. pAC-EHER

    Plasmid
    #53262
    Purpose
    Contains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, idi, crtX, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53262-plasmid-map-sequence-id-82988

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  50. pWJB9

    Plasmid
    #126650
    Purpose
    Expresses AdrA from a modified pLAFR3
    Depositor
    Ute Romling
    Article
    Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d
    Insert
    adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
    Use
    Tags
    8xHis
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126650-plasmid-map-sequence-id-252591

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Showing: 1751 - 1800 of 2321 results