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We narrowed to 59,662 results for: plasmid

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Showing: 701 - 720 of 59662 results

  1. pX330 Pten

    Plasmid
    #59909
    Purpose
    pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter
    Depositor
    Tyler Jacks
    Publication
    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting Pten (Pten Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59909-plasmid-map-sequence-id-123315

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  2. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40

    Plasmid
    #105551
    Purpose
    AAV expression of GFP-Cre from CamKII promoter
    Depositor
    James M. Wilson
    Has service
    AAV9 and AAV Retrograde
    Insert
    EGFP-Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CamKII
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105551-plasmid-map-sequence-id-202479

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  3. pSFV3

    Plasmid
    #92072
    Purpose
    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.
    Depositor
    Henrik Garoff
    Publication
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Type
    Empty backbone
    Use
    Empty expression plasmid, for run-off transcripti…
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92072-plasmid-map-sequence-id-181621

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  5. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Publication
    van Voorst et al bioRxiv [Preprint]. 2025 Jul 7:2025.06.3
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and pTRE3G-bidirectional
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    182307-plasmid-map-sequence-id-516841

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  10. pCBC-DT1T2

    Plasmid
    #50590
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, U626t plus, U629p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50590-plasmid-map-sequence-id-210724

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  11. MSP2133

    Plasmid
    #72249
    Purpose
    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    Y450A, N497A, R661A, Q695A, and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72249-plasmid-map-sequence-id-131869

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  12. T7-VEE-OKS-iM

    Plasmid
    #58972
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Publication
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter and IRES
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58972-plasmid-map-sequence-id-466890

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  14. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Publication
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46569-plasmid-map-sequence-id-364492

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  15. pUCM-AAVS1-TO-hNGN2

    Plasmid
    #105840
    Purpose
    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron
    Depositor
    Michael Ward
    Publication
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105840-plasmid-map-sequence-id-472335

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  16. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Expression
    Insect
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112645-plasmid-map-sequence-id-217990

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  18. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Publication
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105829-plasmid-map-sequence-id-203267

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Showing: 701 - 720 of 59662 results