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20,146 results

Showing: 11161 - 11190 of 20146 results

  7. pGNW2

    Plasmid
    #122086
    Purpose
    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
    Depositor
    Pablo Ivan Nikel
    Article
    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    122086-plasmid-map-sequence-id-451911

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  9. pRSET-RCaMP1h

    Plasmid
    #42874
    Purpose
    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby
    Depositor
    Loren Looger
    Article
    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33
    Insert
    Red Fluorescent Calcium binding Protein
    Tags
    EK (Enterokinase) Recognition Site, His Tag (6x),…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42874-plasmid-map-sequence-id-58020

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  13. pNO286-3

    Plasmid
    #107746
    Purpose
    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)
    Depositor
    Jeffrey Tabor
    Article
    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb
    Inserts
    CcaS
    Ho1
    PcyA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Deleted AA 223 and 512
    Promoter
    J23106 and J23108
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107746-plasmid-map-sequence-id-209202

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  15. pcDNA3-cdk5GFP

    Plasmid
    #1346
    Depositor
    Li-Huei Tsai
    Insert
    CDK5 (CDK5 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 13, 2006
    Availability
    Academic Institutions and Nonprofits only
    1346-plasmid-map-sequence-id-156236

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  17. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71667-plasmid-map-sequence-id-243369

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  20. pIRES Centrin1 mCherry

    Plasmid
    #64338
    Purpose
    Bicistronic expression of mCherry-Centrin1 fusion and Hygro resistance gene
    Depositor
    Matthieu Piel
    Insert
    Centrin1
    Tags
    IRES-Hygro and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64338-plasmid-map-sequence-id-227446

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  22. MCS+

    Plasmid
    #90412
    Purpose
    A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane
    Depositor
    Katharina Gaus
    Article
    Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103
    Insert
    Lck10+ Venus+mcherry+ strech of positively charged amino acids
    Expression
    Mammalian
    Available Since
    May 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    90412-plasmid-map-sequence-id-178958

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Showing: 11161 - 11190 of 20146 results