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Showing: 1901 - 1950 of 6475 results

  1. AAV-U6-gRNA-TnT-Cre

    Plasmid
    #132551
    Purpose
    For loss-of-function experiments in cardiomyocytes
    Depositor
    William Pu
    Inserts
    TnT-Cre
    U6-gRNA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    TnT and U6
    Available since
    Oct. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    132551-plasmid-map-sequence-id-258601

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  4. AAV-hSyn-Ribo-jGCaMP8m

    Plasmid
    #167574
    Purpose
    DEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Has Service
    AAV1
    Insert
    Ribo-GCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167574-plasmid-map-sequence-id-334462

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  16. pAAV-CAG-FLEX-EGFP

    Plasmid
    #59331
    Purpose
    Expresses EGFP
    Depositor
    Ian Wickersham
    Has Service
    AAV1
    Insert
    EGFP
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59331-plasmid-map-sequence-id-468735

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  21. AAV-KrasG12C/sgKras/Cre

    Plasmid
    #99850
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99850-plasmid-map-sequence-id-193772

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  24. pAAV-UbC-mCherry Rab5

    Plasmid
    #115239
    Purpose
    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.
    Depositor
    Pantelis Tsoulfas
    Insert
    Rab5a (RAB5A Homo sapiens, Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Human UbC
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    115239-plasmid-map-sequence-id-222083

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  27. pODN-AAVS1-mNG-CAAX

    Plasmid
    #183870
    Purpose
    Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183870-plasmid-map-sequence-id-362419

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  28. pAAV2-CAGIG-H2B-V5-mScarlet

    Plasmid
    #191093
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    H2B and mScarlet
    Use
    AAV
    Tags
    H2B-V5-mScarlet
    Expression
    Mammalian
    Mutation
    The fusion protein was optimized to the Human cod…
    Promoter
    CMV and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191093-plasmid-map-sequence-id-380126

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  30. pAAV hSyn1 YFP-CaRhAC

    Plasmid
    #101721
    Purpose
    Yellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase created from the rhodopsin guanylyl cyclase of Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellu
    Depositor
    Thomas Oertner
    Article
    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:
    Insert
    YFP CaRhAC
    Use
    AAV
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    101721-plasmid-map-sequence-id-201305

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Showing: 1901 - 1950 of 6475 results