We narrowed to 12,778 results for: NUC
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Plasmid#221077PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertCCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221079PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertTTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE
Plasmid#138145PurposeExpresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.DepositorInsertPspCas13b
UseLentiviralTagsNLSAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-Turbo-YFP-NLS-STOP-L2
Plasmid#127361Purposegateway entry vector for expressing nuclear TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGH00.0126
Plasmid#64257PurposeBinary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformationDepositorInsertsEGFP
neomycin phosphotransferase II
UseBinary agrobacterium vector for plant transformat…ExpressionPlantPromoterE12-omegaAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.4.hSyn.H2B.RFP
Plasmid#170387PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.DepositorInsertH2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBRed-d2-hygro
Plasmid#139196Purposenon-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puroDepositorInsertCBRed
UseLuciferase and RetroviralTagsd2 PEST domain and nuclear localization signal (N…ExpressionMammalianAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-Erk2
Plasmid#50848PurposeFluorescent reporter for Erk nuclear translocationDepositorAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSS146
Plasmid#64770PurposeFor integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminatorDepositorInsertCre-EBD78
Tagsfused to the human Estrogen Binding Domain (EBD),…ExpressionYeastPromoterTDH4Available SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-LaminB1-10
Plasmid#58020PurposeLocalization: Nuclear Envelope, Excitation: 555, Emission: 584DepositorAvailable SinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR_L1-YFP-miniTurbo-NES-L2
Plasmid#127351Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-Turbo-NES-L2
Plasmid#127349Purposegateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
GAG-PEmax V7
Plasmid#232921PurposeOptimized construct for the expression of PEmax fused to GAG from fMLV. This plasmid is involved in the production of NanoScribesDepositorInsertGAG FMLV fused to PEmax
UseCRISPRTagsNuclear export signals (x3)ExpressionMammalianAvailable SinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75154PurposeNuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC6-pA
Plasmid#220758PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC6
UseAAVPromoterCAGAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a TM-ER-delta DB
Plasmid#8354DepositorInsertFOXO3a (FOXO3 Human)
TagsHAExpressionMammalianMutationT32A, S253A, S315A, ER (G525R), delta DB (deletio…Available SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28b_RanT24N
Plasmid#186271PurposeExpression vector of His-RanT24N in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
TagsHis tagged and His-tagged at N terminalExpressionBacterialMutationChanged T24 into NPromoterT7 promoterAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC4-pA
Plasmid#220756PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC4
UseAAVPromoterCAGAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC5-pA
Plasmid#220757PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC5
UseAAVPromoterCAGAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2
Plasmid#182357PurposePositive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.DepositorInsertGFP
ExpressionWormPromoterPeft-3Available SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-AgDD
Plasmid#80625Purposecreate nuclear protein aggregates in mammalian cellsDepositorAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-NFIX2
Plasmid#112699PurposeExpression of NFIX2 protein and GFPDepositorAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB
Plasmid#78348PurposeExpresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-KRAB
UseSynthetic BiologyTagsrTetRExpressionMammalianPromoterpEF alphaAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-VdC9BV
Plasmid#62195PurposeExpresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous GenesDepositorInsertVP64dCas9BFPVP64
UseLentiviralTagsTwo VP64s tagged to dCas9 fused to BFPExpressionMammalianMutationD10A H840A (catalytically inactive) Cas9 (dCas9)Available SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
SLF155: AAV-syn-DO-H2B-jRGECO1a
Plasmid#112278PurposeFor AAV based expression of nuclear jRGECO1a in neurons in Cre-negative neurons with FLEx switchDepositorInsertH2B-jRGECO1a
UseAAV and Cre/LoxExpressionMammalianPromoterhSynAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-NRBF2
Plasmid#99330PurposeMammalian expression of NRBF2, which associates with the PI3KC3-C1 complexDepositorInsertNuclear receptor-binding factor 2 (NRBF2 Human)
TagsHis-MBPExpressionBacterialPromoterT7Available SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-VPS35
Plasmid#21689DepositorInsertVacuolar Protein Sorting 35 homolog (VPS35 Human)
UseGateway entry cloneMutationlast nucleotide of stop codon removed for c-termi…Available SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEY43
Plasmid#191047Purposeeat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMVhPOP1_3Flag
Plasmid#53968PurposeMammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.DepositorAvailable SinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only