20,146 results

Showing: 11731 - 11760 of 20146 results

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MARIO/ pcDNA3

Plasmid

#124838

Purpose

mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

Depositor

Takeharu Nagai

Article

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

MARIO

Tags

No tag

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKD1

Plasmid

#23500

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKD1 (PRKD1 Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FNIP1

Plasmid

#72292

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP1 (FNIP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRRL MND GFP

Plasmid

#36247

Depositor

David Rawlings

Article

Astrakhan et al Blood. 2012 Mar 19.

Insert

GFP (eGFP )

Use

Lentiviral

Promoter

MND

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pMIG-IRF4

Plasmid

#58987

Purpose

MSCV based expression vector - co-expresses GFP and murine IRF4

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Insert

Mouse IRF4 (Irf4 Mouse)

Use

Retroviral

Tags

Kozak sequence added

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CACNA1A KI

Plasmid

#131480

Purpose

Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1a, Cacna1a Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS5A

Plasmid

#17646

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS5A (POLY Hepatitis C virus)

Tags

Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pMSCVsin-PGK-GFP

Plasmid

#35615

Depositor

Patrick Salmon

Insert

GFP

Use

Retroviral

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pHR'-NRSF-CITE-GFP

Plasmid

#21310

Depositor

Jay Nadeau

Article

Nadeau et al J Neurophysiol. 2002 Jul . 88(1):409-21.

Insert

neuron restrictive silencer factor (Rest Mouse)

Use

Lentiviral

Tags

GFP (bicistronic with CITE/IRES)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Oct. 20, 2009

Availability

Academic Institutions and Nonprofits only

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pC-HBH

Plasmid

#88896

Purpose

Carries phiC31 integrase attB site flanked by cHS4 insulators.

Depositor

Michele Calos

Article

Zhao et al PLoS One. 2014 Apr 29;9(4):e96279. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pC001 - huLshC2C2-MBP for bacterial expression

Plasmid

#79150

Purpose

Expresses human codon-optimized LshC2c2 for purification in E. coli

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

C2c2

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-P25C-GFP

Plasmid

#1343

Depositor

Li-Huei Tsai

Insert

P25 (CDK5R1 Human)

Tags

GFP

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_CoV2_P1

Plasmid

#170450

Purpose

Express SARS-CoV-2 P.1 (Brazil / gamma) strain spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 P.1 pseudotyped virus production.

Depositor

David Nemazee

Article

Yuan et al Science. 2021 Aug 13;373(6556):818-823.

Insert

SARS-CoV-2 P.1 spike D18 (S SARS-CoV-2, P.1 strain)

Tags

NA

Expression

Mammalian

Mutation

L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

Promoter

CMV

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneo-hCHD7

Plasmid

#89458

Purpose

constitutive expression of human CHD7 in mammalian cells

Depositor

Peter Scacheri

Insert

CHD7 (CHD7 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Hygromycin-sfGFP-MAP

Plasmid

#44100

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Tags

sfGFP-MAP

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-nv5-ER beta

Plasmid

#22770

Depositor

Donald McDonnell

Article

Wittmann et al Cancer Res. 2007 Oct 1. 67(19):9549-60.

Insert

estrogen receptor beta (ESR2 Human)

Tags

v5

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-GFP-HiBiT

Plasmid

#198317

Purpose

Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-GFP-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV SOCS-3

Plasmid

#11486

Depositor

Ronald Kahn

Article

Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

Insert

SOCS-3 (Socs3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-EGFR

Plasmid

#23935

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EGFR (EGFR Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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YAP1 (S6A) - V5 in pLX304

Plasmid

#42562

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S61A, S109A, S127A, S128A, S131A, S163A, S164A, S…

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMVtight Blast DEST (w762-1)

Plasmid

#26434

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Expression

Mammalian

Available Since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRH2521

Plasmid

#84380

Purpose

Expression of sgRNA from a imyc promoter (Pimyc)

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

sgRNA cloning site

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF1-3'UTR

Plasmid

#136036

Purpose

UPF1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (TTATTACCCAGAATAAGATGC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe RFP1-KRT5 hygro

Plasmid

#58493

Purpose

mammalian expression of mRFP1-tagged human KRT5

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

KRT5 (KRT5 Human)

Tags

mRFP1

Expression

Mammalian

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
pPAmCherry-b-actin

Plasmid

#31949

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

b-actin

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

CMV

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pBudORF2-CH

Plasmid

#51289

Purpose

expresses the codon optimized ORF2 of L1RP with his tag

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

Insert

L1 ORF2 (L1RE1 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-mC/EBPb

Plasmid

#49198

Purpose

expression of mouse C/EBPbeta in mammalian cells

Depositor

Jed Friedman

Insert

C/EBPbeta (Cebpb Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP1

Plasmid

#106408

Purpose

P-RAN-GFP1 (RANbody GFP1::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP1

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-mRuby2 PuroR

Plasmid

#84386

Purpose

Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons

Depositor

Ghassan Mouneimne

Insert

Lifeact

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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20,146 results