We narrowed to 12,778 results for: NUC

Showing: 11761 - 11790 of 12778 results

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mEmerald-H2B-6

Plasmid

#54111

Purpose

Localization: Nucleus/Histones, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

H2B (H2BC11 Human)

Tags

mEmerald

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mRuby2-H2B-6

Plasmid

#55897

Purpose

Localization: Nucleus/Histones, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

H2B (H2BC11 Human)

Tags

mRuby2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

Plasmid

#149426

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pHBS1413 GFP-TDP43 VLIM-F

Plasmid

#118802

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Mutation

All VLIM to F in CTD of full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179052

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SRM1-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75177

Purpose

Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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mOrange2-LaminB1-10

Plasmid

#57966

Purpose

Localization: Nuclear Envelope, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LaminB1 (LMNB1 Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3/HIP1/ΔLD

Plasmid

#31253

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Expression

Bacterial

Mutation

Deltion of nucleotides 767-1335

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3/HIP1/ΔC

Plasmid

#31250

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Expression

Bacterial

Mutation

Deltion of nucleotides 1336-2513 1386bp C to T…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pET28b(+)-SPN+IFS

Plasmid

#83372

Purpose

Expresses SPN and IFS complex in E. coli cell

Depositor

SeWon Suh

Article

Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

Inserts

SPN
IFS

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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3xFN-Tel-TAL/pCMV-7.1

Plasmid

#63589

Purpose

Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

Insert

3xFVN-Tel-TAL

Use

TALEN

Tags

3xFLAG-tag, V5-tag, and nuclear localization sign…

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-Nvec-codonopti-Cas9-2xNLS

Plasmid

#141108

Purpose

Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

Depositor

Matthew Gibson

Article

Hill et al Methods Mol Biol. 2022;2450:437-465. doi

Insert

Nematostella codon optimized Cas9

Use

Unspecified

Mutation

All amino acids coded for by codons optimized for…

Promoter

T7

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Fibrillarin-7

Plasmid

#58016

Purpose

Localization: Nucleoli, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Fibrillarin (FBL Human)

Tags

mTagRFP

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pHBS1411 GFP-TDP43 FYW-S

Plasmid

#118800

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Mutation

All FYW to S in CTD of full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1415 GFP-TDP43 VLIM-S

Plasmid

#133323

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Mutation

ALL VLIM in the TDP43 IDRs mutated to S

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMALp2x-SCO5461(43-204)

Plasmid

#42509

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Tags

maltose-binding protein

Expression

Bacterial

Mutation

deleted amino acids 1-42 (nucleotide 1-126)

Promoter

tac

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
BII-gR-PtW-eSpCas9

Plasmid

#133357

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

HA-tagged eSpCas9

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA-EF1BFP

Plasmid

#111144

Purpose

Expression of mouse Trex2 and BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

mouse Trex2 coding region (Trex2 Mouse)
BFP (Rnf112 )

Expression

Mammalian

Promoter

CAG and EF1

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-H2B-C-10

Plasmid

#57804

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Tags

tdminiSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

Plasmid

#127365

Purpose

Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Tags

GS linker, NLS, V5, and YFP

Expression

Plant

Mutation

R118G

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-DN-CMV-GFP

Plasmid

#83252

Purpose

Adenoviral expression of STAT5A dominant negative with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Mutation

C-terminal deletion of 135 nucleotides (dominant …

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Gcm2TEASY

Plasmid

#41031

Depositor

Nancy Manley

Article

Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

Insert

Gcm2 in situ probe (Gcm2 Mouse)

Use

In situ probe

Mutation

contains nucleotides 584-1510 of GenBank accessio…

Promoter

T7 sense; SP6 antisense

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pEY61

Plasmid

#191058

Purpose

acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-2(3.7k) (acr-2 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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sox10:PDGFRA wild-type

Plasmid

#98696

Purpose

I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

PDGFRA (PDGFRA Human)

Use

Zebrafish expression

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-DNL-CMV-GFP

Plasmid

#83253

Purpose

Adenoviral expression of STAT5A dominant negative with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5A (Stat5a Mouse)
eGFP

Use

Adenoviral

Mutation

C-terminal deletion of 351 nucleotides (dominant …

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1412 GFP-TDP43 FYW-L

Plasmid

#118801

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Mutation

All FYW to L in CTD of full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pp32ΔCT

Plasmid

#67241

Purpose

pp32(1-149) cloned into pProEX HTb

Depositor

Cynthia Wolberger

Article

Huyton et al Protein Sci. 2007 Jul;16(7):1308-15. Epu

Insert

pp32 (ANP32A Human)

Tags

His

Expression

Bacterial

Mutation

contains amino acid residues 1-149

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pLV.U6gT28.13.EFS-NS.H2B-RFP

Plasmid

#170388

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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p35S::IRE1a

Plasmid

#135232

Purpose

constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

IRE1a (IRE1A Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

Plasmid

#118279

Purpose

Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

Depositor

Dietmar Schmitz

Article

Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

Inserts

mRuby2
ChR2(H134R)

Use

AAV and Cre/Lox

Tags

SV40 NLS and YFP

Promoter

EF1 alpha

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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