161,407 results

1. FASTHDR-tRNApromo-sgRNA-espCas91_1

   Plasmid

   #167210

   Purpose

   Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100

   Plasmid

   #187584

   Purpose

   Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SP100 (SP100 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV Myc T58A puro

   Plasmid

   #18773

   Depositor

   Scott Lowe

   Article

   Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

   Insert

   myc T58A (MYC Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T58A

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-OptoSTIM1

   Plasmid

   #70159

   Purpose

   Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

   Depositor

   Won Do Heo

   Article

   Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

   Insert

   OptoSTIM1 (STIM1 Human, Mustard Weed)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCaSpeR4-tubulin-QF#7

   Plasmid

   #46127

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF#7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYES2/103Q

   Plasmid

   #1385

   Depositor

   Michael Sherman

   Article

   Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

   Insert

   huntingtin (103Q) (HTT Human)
   

   Use

   Tags

   EGFP and FLAG

   Expression

   Yeast

   Mutation

   Exon1 sequences containing the first 17 amino aci…

   Promoter

   Available since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Pur-CAG-mCherry

   Plasmid

   #80946

   Purpose

   human AAVS1 site targeting donor plasmid for knocking-in mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-mfap4

   Plasmid

   #70052

   Purpose

   5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Insert

   mfap4 promoter
   

   Use

   Gateway 5' element

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

   Plasmid

   #20299

   Purpose

   Cre-activated AAV expression of mCherry

   Depositor

   Karl Deisseroth

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. p40PX-EGFP

    Plasmid

    #19010

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p40phox (NCF4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-148 (PX domains)

    Promoter

    CMV

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLENTI_TetOn_Hu_BIRC5(WT)

    Plasmid

    #136348

    Purpose

    Lentiviral inducible expression (TetOn) of human BIRC5

    Depositor

    Guillermo de Cárcer

    Insert

    BIRC5 (BIRC5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF6-Acly

    Plasmid

    #70765

    Purpose

    Expresses mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Use

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. HaloTag-sec61b

    Plasmid

    #186959

    Purpose

    Express sec61b connected with N-terminus HaloTag

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Sec61 (SEC61B Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sox1/2/3-2373/+3>nls::Cas9::nls

    Plasmid

    #59989

    Purpose

    Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Sox1/2/3 (SoxB1) -2373/+3

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-hPMCA4b

    Plasmid

    #47589

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA4x/b (ATP2B4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K442R and P673L relative to NP_001675.3

    Promoter

    CMV

    Available since

    Aug. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCT310

    Plasmid

    #111140

    Purpose

    Bacterial expression of 6His-dCas9-3XFlag

    Depositor

    Robert Tjian

    Article

    Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

    Insert

    dCas9
    

    Use

    Tags

    3X Flag and 6X His

    Expression

    Bacterial

    Mutation

    D10A - H840A

    Promoter

    T7

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBSTNAV

    Plasmid

    #45801

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV(MinDis).iGluSnFR

    Plasmid

    #41732

    Purpose

    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Insert

    GltI based Glutamate Biosensor
    

    Use

    Glutamate biosensor

    Tags

    Myc, PDGFR transmembrane helix (aa 513-561), and …

    Expression

    Mammalian

    Mutation

    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

    Promoter

    CMV

    Available since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCF1148

    Plasmid

    #222330

    Purpose

    Fluorescent reporter plasmid for Veillonella

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Insert

    bs2 gene with Veillonella parvula SKV38 mdh promoter
    

    Use

    E. coli-veillonella shuttle plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Veillonella parvula SKV38 mdh promoter

    Available since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pTwist-SARS-CoV-2 Spike-deltaC18 LB.1

    Plasmid

    #223277

    Purpose

    Encodes SARS-CoV-2 spike LB.1 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike LB.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. AAV-Cre-GFP

    Plasmid

    #68544

    Purpose

    AAV expression of Cre-GFP fusion

    Depositor

    Eric Nestler

    Article

    Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. TVT7R(0,0)

    Plasmid

    #98631

    Purpose

    Vector for cloning infectious RNA virus genomes

    Depositor

    Andrew Ball, Karyn Johnson

    Article

    Johnson et al J Virol. 2000 Jun;74(11):5123-32.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBR322-TIMER

    Plasmid

    #103056

    Purpose

    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

    Depositor

    Dirk Bumann

    Article

    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

    Insert

    TIMER-bac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations: S4T and S179T

    Promoter

    constitutive ybaJp promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pDIRECT_22A

    Plasmid

    #91133

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3-His:PYL1:nls:VPR

    Plasmid

    #135988

    Purpose

    Expresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    PYL1 domain fused to the VPR activation domain
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAC574

    Plasmid

    #127975

    Purpose

    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    hygB (hygromycin resistance marker)
    

    Use

    Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pUC19-link-mCherry-FLAG:cat

    Plasmid

    #211831

    Purpose

    Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mCherry-FLAG-cat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCIG3 N

    Plasmid

    #132941

    Purpose

    MLV packaging plasmid for N-tropic MLV gag-pol expression

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    N-tropic MLV gag-pol
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAVdual-CMV-eGFP

    Plasmid

    #230931

    Purpose

    pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter.

    Depositor

    Qizhao Wang

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. S-HBsAg

    Plasmid

    #103013

    Purpose

    Expresses HBV Small envelope glycoprotein in mammalian cells

    Depositor

    Wang-Shick Ryu

    Article

    Wang et al PLoS Pathog. 2010 Jul 15;6(7):e1000986.

    Insert

    HBV Small envelope protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge