178,769 results
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Plasmid#107008PurposeExpresses epitope-tagged wild-type human TTPDepositorAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
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RNA motif plasmid EDEN15
Plasmid#107252PurposeEDEN15 RNA motifDepositorInsertEDEN15 motif
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ132B2.0
Plasmid#99888PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pmGFP-P2A-K(AAA)12-P2A-RFP
Plasmid#105687PurposeDouble fluorescent stalling reporter K(AAA)12DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsP2A-3xFLAG-VHPbeta
K(AAA)12
P2A-mCherry
ExpressionMammalianPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRluc-LC3G120A
Plasmid#105003PurposeMammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLC3B (Map1lc3b Rat)
UseLuciferaseTagsRluc with C124A substitutionExpressionMammalianMutationGlycine 120 mutated to alanine, prevents lipidati…Available sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-Cre-T2A-mRuby2
Plasmid#102989PurposeExpresses Cre and mRuby2DepositorInsertsCre (site-specific recombinase)
mRuby2
UseCre/LoxExpressionMammalianPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS_L_EBOV
Plasmid#103052PurposeEncodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorAvailable sinceDec. 20, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pAAV-CMV-FLEX-TVAmCherry-2A-oG
Plasmid#102985PurposeAAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombinationDepositorInsertsTVA-mCherry fusion protein after CRE-mediated recombination
Optimised Rabies Glycoprotein (oG) after CRE-mediated recombination
UseAAV; Adeno associated viral vectorAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Kif5A-GFP-CIBN
Plasmid#102252PurposeExpresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER dCas9-TET1CDMut
Plasmid#101920PurposeThe dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.DepositorInsertdCas9-TET1CD Mut
UseLentiviralExpressionMammalianMutationMutated TET1 catalytic domainAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPPAT-EGFP
Plasmid#99105PurposeExpresses human PPAT-EGFP fusion in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPPAT (PPAT Human)
TagsEGFP (enhanced green fluorescent protein)ExpressionMammalianPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSAD-F3-NPEST-FLPo-2A-mCherryPEST
Plasmid#99609PurposeSelf-inactivating DG-Rabies (SiR) expressing FLPo and destabilised mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsFLPo
mCherry
UseUnspecifiedTagsPESTAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-puro-2A-TEVp
Plasmid#99610PurposeLentivirus expressing puromycin resistance gene and TEV proteaseDepositorInsertsPuromycin resistance (PAC gene)
TEV protease
UseLentiviralAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BMP4_pLX307
Plasmid#98321PurposeLentiviral expression of BMP4DepositorAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry
Plasmid#85430Purpose[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expressionDepositorInserthuman IL-10 T2A PD-L1
UseLentiviralExpressionMammalianAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV
Plasmid#91719PurposeAAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2DepositorInsertmEGFP-P2A-paAIP2(R5AR6A)
UseAAVPromoterCaMKII 1.3Available sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GFP
Plasmid#86537PurposeRetrovirus expressing GFPDepositorInsertGFP
UseRetroviralExpressionMammalianPromoterSynapsin I (human)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMyC-kan
Plasmid#84692PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAcetamidaseAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mtagBFP
Plasmid#89685PurposepCAG promoter driving mtagBFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmtagBFP
UseSynthetic BiologyExpressionMammalianAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2
Plasmid#87694PurposeExpress ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERT2-PhoCl-Cre-PhoCl-ERT2
ExpressionMammalianPromoterChicken beta actinAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psMEK1tight
Plasmid#89362PurposeA lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cellsDepositorInsertpsMEK1tight (MAP2K1 Human, Synthetic)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mTubb3
Plasmid#87116PurposeAAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITIDepositorInsertU6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
UseAAV and CRISPRAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3_V5-APEX2-Sec61B
Plasmid#83412PurposeExpressed N-term tagged APEX2 on Sec61B in mammalian cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertV5-APEX2-Sec61B (SEC61B Human, Synthetic)
TagsAPEX2 and V5ExpressionMammalianPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT G250A
Plasmid#84925Purposeluciferase reporter for mutant TERT promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationG250APromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1
Plasmid#86885PurposeC-terminal part of the split-BioIDDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBirA* (AA141-321)- HA- NIPP1]
TagsHA tagExpressionMammalianMutationBirA Amino Acids 141-321Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_PAsfCherry2(1-10)
Plasmid#82612PurposeExpress PAsfCherry2(1-10) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPAsfCherry2(1-10)
ExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterCMVAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
TagsAmilCP and RTXExpressionBacterialPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Dazl-CPG-TV
Plasmid#70144Purposea targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
UseMouse Targeting and Synthetic BiologyAvailable sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)
Plasmid#85041PurposeThis plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertloxP-stop-loxP
UseCRISPRExpressionMammalianAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only