Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

172,829 results

Showing: 11941 - 11970 of 172829 results

  6. mCherry-7aa-cb5-pEGFP-C1

    Plasmid
    #177407
    Purpose
    To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177407-plasmid-map-sequence-id-348154

    Enlarge

  7. LSB-hsa-miR-192-3p

    Plasmid
    #103303
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-192-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-192-3p target (MIR192 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103303-plasmid-map-sequence-id-195655

    Enlarge

  8. LSB-hsa-miR-192-5p

    Plasmid
    #103304
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-192-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-192-5p target (MIR192 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103304-plasmid-map-sequence-id-195657

    Enlarge

  12. pET-Impact-T4gp41

    Plasmid
    #228207
    Purpose
    Expression in BL21(DE3) of T4 helicase
    Depositor
    Stephen Benkovic
    Insert
    41 (41 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228207-plasmid-map-sequence-id-453945

    Enlarge

  13. pET-Impact-T4gp59

    Plasmid
    #228206
    Purpose
    Expression in BL21(DE3) of WT T4 helicase loader
    Depositor
    Stephen Benkovic
    Insert
    59 (59 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228206-plasmid-map-sequence-id-453944

    Enlarge

  22. EB1-tdTomato

    Plasmid
    #50825
    Purpose
    expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs
    Depositor
    Erik Dent
    Insert
    EB1 (Mapre1 Mouse)
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    50825-plasmid-map-sequence-id-377726

    Enlarge

  25. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

    Enlarge

  27. pAAV.hSyn.iGluSnFR3.v857.SGZ

    Plasmid
    #178330
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn iGluSnFR3 v857.SGZ
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…
    Expression
    Mammalian
    Available Since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    178330-plasmid-map-sequence-id-353668

    Enlarge

  28. AICSDP-64: FUS-mEGFP

    Plasmid
    #114410
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FUS
    Depositor
    Allen Institute for Cell Science
    Insert
    FUS Homology Arms with linker-mEGFP (FUS Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 5, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114410-plasmid-map-sequence-id-220875

    Enlarge

Showing: 11941 - 11970 of 172829 results