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Showing: 1951 - 2000 of 6475 results

  3. AAV-KrasG12V/sgKras/Cre

    Plasmid
    #99854
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99854-plasmid-map-sequence-id-193790

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  4. pAAV-EFS-DIO-GRAB_CRFmut

    Plasmid
    #208663
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent manner
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Oct. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    208663-plasmid-map-sequence-id-412146

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  11. pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid
    #105983
    Purpose
    This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
    Depositor
    Marcel Bruchez
    Article
    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19
    Insert
    FLEX-SYFP2-POST-T2A-dTomato.FLEX
    Use
    AAV and Cre/Lox
    Tags
    SYFP2-POST, T2A-dTomato, and myc
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin 1
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    105983-plasmid-map-sequence-id-235283

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  17. AAV-EF1a-jGCaMP8m-WPRE

    Plasmid
    #176757
    Purpose
    AAV-mediated expression of GCaMP8m under the EF1a promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    176757-plasmid-map-sequence-id-351328

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  20. AAV-SYN-TdTomato-RAB11

    Plasmid
    #203732
    Purpose
    AAV vector plasmid expressing human RAB11 fused to TdTomato under the human synapsin (SYN) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Insert
    RAB11 (RAB11A Human)
    Use
    AAV
    Tags
    TdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Human synapsin promoter
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203732-plasmid-map-sequence-id-402639

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  21. pAAV-UbC-mEmerald Rab7a

    Plasmid
    #115238
    Purpose
    To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.
    Depositor
    Pantelis Tsoulfas
    Insert
    Rab7a fused to mEmerald (RAB7A Human)
    Use
    AAV
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    7 Amino Acids (21 Nucleotides) between mEmerald a…
    Promoter
    Human UbC
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    115238-plasmid-map-sequence-id-222080

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  23. pAAV/EF1a-DIO-SCLM

    Plasmid
    #216759
    Purpose
    AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    EF1a (human elongation factor 1 alpha)
    Available since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    216759-plasmid-map-sequence-id-429631

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  29. pAAV-synp-F-synaptophysin-jRGECO1a

    Plasmid
    #102997
    Purpose
    Expresses synaptophysin fused jRGECO1a under the synapsin promoter
    Depositor
    David Cox
    Insert
    Synaptophysin-jRGECO1a
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    102997-plasmid-map-sequence-id-199761

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  30. pAAV-tMCK-Cyto-TurboID

    Plasmid
    #149411
    Purpose
    Muscle-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection
    Depositor
    Jonathan Long
    Insert
    Cyto-TurboID
    Use
    AAV
    Tags
    V5
    Expression
    Mutation
    Promoter
    tMCK
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149411-plasmid-map-sequence-id-299815

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  31. pAAV-FLEXfrt-mCherry-KASH

    Plasmid
    #209200
    Purpose
    Flp-dependent expression of mCherry tagged KASH protein domain
    Depositor
    Larry Zweifel
    Insert
    mCherry-KASH
    Use
    AAV
    Tags
    KASH localization domain
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    209200-plasmid-map-sequence-id-418197

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  33. pAAV.CAG-FLEX.iAChSnFR-Venus-NULL

    Plasmid
    #137960
    Purpose
    Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (non-binding yellow variant)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Y140A binding site mutation
    Promoter
    CAG
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137960-plasmid-map-sequence-id-271575

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  36. AAV-hSyn-Ribo-jGCaMP8f

    Plasmid
    #167573
    Purpose
    DEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Insert
    Ribo-GCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167573-plasmid-map-sequence-id-334459

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  40. pAAV-DCX(S306D)-2A-mCherry

    Plasmid
    #118292
    Purpose
    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    Doublecortin (DCX Human)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Mutation
    Serine 306 changed to Aspartic Acid
    Promoter
    CMV with beta global intron
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118292-plasmid-map-sequence-id-229506

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  41. pAAV-DCX(S306A)-2A-mCherry

    Plasmid
    #118291
    Purpose
    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanine
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    Doublecortin (DCX Human)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Mutation
    changed Serine 306 to Alanine
    Promoter
    CMV with beta global intron
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118291-plasmid-map-sequence-id-229504

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  45. pAAV hSyn-Flex RG-cerulean

    Plasmid
    #98221
    Purpose
    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus
    Depositor
    Troy Margrie
    Insert
    Cerulean-E2A-RG
    Use
    AAV
    Tags
    cerulean
    Expression
    Mutation
    Promoter
    human Synapsin
    Available since
    Aug. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98221-plasmid-map-sequence-id-192715

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Showing: 1951 - 2000 of 6475 results