We narrowed to 9,980 results for: Uty

1. pCRISP_IS

   Plasmid

   #120425

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   constitutive

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p35S::mCh-HA-P2A-lifeact-mCh

   Plasmid

   #135234

   Purpose

   constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

   Depositor

   Armin Djamei

   Article

   Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

   Insert

   mCh-HA-P2A-lifeact-mCh
   

   Tags

   HA, lifeact, and p2a

   Expression

   Plant

   Promoter

   35S

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSM17

   Plasmid

   #101641

   Purpose

   Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   eqFP486
   

   Tags

   6XHis

   Expression

   Bacterial

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p414GPD-3xFLAG-Nop4 WT

   Plasmid

   #169261

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 WT (NOP4 Budding Yeast)
   

   Tags

   3xFLAG

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSM12

   Plasmid

   #101643

   Purpose

   Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   meffCFP
   

   Tags

   6XHis

   Expression

   Bacterial

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCEP4-myc-H2-Ld

   Plasmid

   #135519

   Purpose

   Mammalian expression of myc-tagged H2-Ld

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   H2-Ld
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJB001 CMV 3xFLAG-NLS-FKBP-ZF2

   Plasmid

   #161545

   Purpose

   Constitutive expression of 3xFLAG-NLS-FKBP-ZF2 under the CMV promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-FKBP-ZF2
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1V5-His Snk/Plk2 D223N

   Plasmid

   #22218

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Expression

   Mammalian

   Mutation

   single amino acid substitution mutant, aspartic a…

   Available Since

   Aug. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pMRE151

   Plasmid

   #118501

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Expression

   Bacterial

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJH131

    Plasmid

    #48260

    Purpose

    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    GAL10/GAL1 bidirectional promoter
    

    Use

    Routine cloning vector

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    GAL10/GAL1 bidirectional

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PhyB-mCherry-SIK1

    Plasmid

    #51577

    Purpose

    Constitutively express nucleolus localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    SIK1 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEAST-J23101-CocE

    Plasmid

    #114600

    Purpose

    "Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    CocE
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Promoter

    J23101

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. R10-3 /pQE-30

    Plasmid

    #79859

    Purpose

    The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.

    Depositor

    Konstantin Lukyanov

    Article

    Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73

    Insert

    R10-3
    

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Aequorea victoria green fluorescent protein (avGF…

    Promoter

    T5 promoter / lac operator

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP

    Plasmid

    #192609

    Purpose

    Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    LexA-CIB1-biLINuS-P2A-tagBFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    tagBFP

    Promoter

    PGK

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PhyB-mCherry-SNF7

    Plasmid

    #51574

    Purpose

    Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    SNF7 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. J23101

    Plasmid

    #55176

    Purpose

    Very weak constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    J23101 promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    J23101

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1 puro shPRDM14-1

    Plasmid

    #193694

    Purpose

    Constitutive lentiviral expression of PRDM14 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1 puro shPRDM14-2

    Plasmid

    #193695

    Purpose

    Constitutive lentiviral expression of PRDM14 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1 puro shPRDM14-3

    Plasmid

    #193696

    Purpose

    Constitutive lentiviral expression of PRDM14 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1 puro shPRDM14-4

    Plasmid

    #193697

    Purpose

    Constitutive lentiviral expression of PRDM14 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge