2,226 results

1. FH95pUp95GW (B4)

   Plasmid

   #74013

   Purpose

   lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

   Depositor

   Weifeng Xu, Oliver Schluter, Robert Malenka

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Insert

   Psd95 shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV Cag Flex H2B Gcamp6s reverse

   Plasmid

   #74157

   Purpose

   Calcium Sensor

   Depositor

   Loren Looger

   Insert

   Gcamp6s
   

   Use

   AAV

   Tags

   H2B

   Expression

   Mutation

   Promoter

   SpeI

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Cx43-YA/VD-GFP

   Plasmid

   #60703

   Purpose

   mammalian expression of rat Cx43 YA/VD mutant fused to GFP

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   YA/VD (Y286A/V289D)

   Promoter

   CMV

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pSuper-Rem2-2

   Plasmid

   #51595

   Purpose

   shRNA 2 against rodent Rem2

   Depositor

   Suzanne Paradis

   Article

   Moore et al PLoS One. 2013 Aug 26;8(8):e74751. doi:

   Insert

   Rem2 (Rem2, Rem2 Mouse, Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pSuper-Rem2-1

   Plasmid

   #51584

   Purpose

   shRNA 1 against rodent Rem2

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2, Rem2 Mouse, Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pLenLox_U6 Nlgn1

   Plasmid

   #59339

   Purpose

   Lentiviral expression vector: Neuroligin 1 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

   Depositor

   Peter Scheiffele

   Article

   Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

   Inserts

   Neuroligin 1 shRNA (rat) (Nlgn1 Rat)
   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and mouse U6

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-parkin 77-465

   Plasmid

   #45970

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin 77-465 (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Dele…

   Promoter

   tac

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRK5-rat PKA-RIIbeta

   Plasmid

   #45522

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA regulatory subunit RII beta
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 AU1 mTOR S2035W

   Plasmid

   #15678

   Depositor

   John Lawrence

   Article

   McMahon et al Mol Cell Biol. 2002 Nov . 22(21):7428-38

   Insert

   mTOR (Mtor Rat)
   

   Use

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   S2035W

   Promoter

   Available since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-N1-wtVHL

    Plasmid

    #13389

    Depositor

    Lucy Anderson

    Article

    Shiao et al Cancer Res. 2000 Jun 1. 60(11):2816-9.

    Insert

    von Hippel-Lindau (Vhl Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS-rat PKA-RIIbeta-mEGFP

    Plasmid

    #45530

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII beta tagged by mEGFP
    

    Use

    With chicken beta actin promoter and wpre

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-Cx43-Myc

    Plasmid

    #60693

    Purpose

    mammalian expression of Myc-tagged rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pRK7-HA-S6K1-F5A-E389

    Plasmid

    #8988

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F5A, T389E

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

14. BE3-NLS-NLS-P2A-BLAST

    Plasmid

    #135348

    Purpose

    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    BE3-2XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXCX.CMV.CREB.WPRE

    Plasmid

    #38050

    Depositor

    James Uney

    Article

    Glover et al Neuroreport. 2004 May 19;15(7):1171-5.

    Insert

    CREB (Creb1 Rat)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSV(X) C/EBPalpha

    Plasmid

    #13392

    Depositor

    Richard Schwartz

    Article

    Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

    Insert

    C/EBPalpha (Cebpa Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pHAGE2-RSV-jGCaMP7f-W

    Plasmid

    #126691

    Purpose

    Mammalian calcium sensor protein.

    Depositor

    Darrell Kotton

    Insert

    jGCaMP7f
    

    Use

    Lentiviral

    Tags

    6xHIS, T7, Xpress

    Expression

    Mutation

    H78K, A317L

    Promoter

    RSV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. RnBarr2-mTurquoise2

    Plasmid

    #137792

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RnBarr1-mTurquoise2

    Plasmid

    #137789

    Purpose

    Visualization of beta-arrestin1

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOC2 CRISPIE-Halo-Homer1 KI

    Plasmid

    #183446

    Purpose

    CreON knock-in for Halo-Homer1 (amino acid position: G2)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and CRISPIE Halo donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSG5-FLAG-CaMKKbeta rat FL

    Plasmid

    #32449

    Depositor

    Anthony Means

    Article

    Anderson et al Cell Metab. 2008 May;7(5):377-88.

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S3L, G96R, and P108L

    Promoter

    SV40

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

22. mApple-CathespinB-6

    Plasmid

    #54871

    Purpose

    Localization: Lysosomes, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    CathespinB
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pGEX-parkin 219-465

    Plasmid

    #45972

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 219-465 (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 7iBi89 (rat fibronectin)

    Plasmid

    #14065

    Depositor

    Richard Hynes

    Article

    Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

    Insert

    Rat Fibronectin 7iBi89 (Fn1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert was cut out of pGEM3 with SalI and SacI an…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pSL007_PB_pCMV-H2B-mIFP-T2A-rTetR-ratKRAB-zeo

    Plasmid

    #179438

    Purpose

    Expresses rTetR-KRAB(rat) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LXSN-Cx43

    Plasmid

    #60692

    Purpose

    retroviral expression of rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pCEP4-HA-MEKK1-1-1493

    Plasmid

    #21632

    Depositor

    Melanie Cobb

    Article

    Xu et al Proc Natl Acad Sci U S A. 1995 Jul 18. 9

    Insert

    mitogen-activated protein kinase kinase kinase 1 (Map3k1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pRB144

    Plasmid

    #180175

    Purpose

    pCDNA3.1 Construct GluN1 A714L

    Depositor

    Richard Bergeron

    Article

    Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

    Insert

    GluN1 (Grin1 Rat)
    

    Use

    Tags

    Phluorin

    Expression

    Mammalian

    Mutation

    A714L

    Promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

    Plasmid

    #26577

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. rNL1(46-638)

    Plasmid

    #20172

    Depositor

    Axel Brunger

    Article

    Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

    Insert

    Neuroligin 1 (Nlgn1 Rat)
    

    Use

    Tags

    C-terminal 6X His tag

    Expression

    Insect

    Mutation

    no splice sites

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only