We narrowed to 7,112 results for: ef1a

1. AAV-EFS-GFP

   Plasmid

   #170176

   Purpose

   Expresses GFP under the control of EF1a short promoter

   Depositor

   Xiaojun Lian

   Article

   Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-mCherry-flex-dtA

   Plasmid

   #58536

   Purpose

   Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

   Depositor

   Naoshige Uchida

   Article

   Wu et al Nature. 2014 May 15;509(7500):325-30. do

   Insert

   dtA (DIP_RS18250 )
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEMS-II-PEST

   Plasmid

   #204766

   Purpose

   GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   EGFP-PEST-m6Alinker-DHFR
   

   Use

   Tags

   Expression

   Mutation

   mutated all RACs in EGFP to RGC/RCC

   Promoter

   CMV

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMB1: All in one SpCas9 expression and sgRNA cloning backbone

   Plasmid

   #228363

   Purpose

   mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF-APEX2-NLS

   Plasmid

   #140281

   Purpose

   Expression of APEX2-NLS

   Depositor

   Yusuke Miyanari

   Article

   Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

   Insert

   APEX2-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. IDP456

   Plasmid

   #87002

   Purpose

   plasmid for cell culture expression of SYNseq components

   Depositor

   Anthony Zador

   Article

   Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

   Insert

   barcode landing site-mCherry-4xboxB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. IDP432

   Plasmid

   #87000

   Purpose

   plasmid for cell culture expression of SYNseq components

   Depositor

   Anthony Zador

   Article

   Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

   Insert

   SNAP-Nlg1AB-1xλN(i)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHIV-H2B-eGFP

   Plasmid

   #91776

   Purpose

   Expresses H2B protein tagged with eGFP

   Depositor

   Maria Pia Cosma

   Article

   Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

   Insert

   Human Histone H2B / eGFP (H2BC11 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

   Plasmid

   #228364

   Purpose

   mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Enascas12a nuclease and en…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p23-DDX21 WT

    Plasmid

    #128803

    Purpose

    overexpression of DDX21 in human cells

    Depositor

    Ling-Ling Chen

    Article

    Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

    Insert

    DDX21 WT
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RAB14 WT

    Plasmid

    #61493

    Purpose

    to overexpress wild type RAB14. Vector includes GFP driven by hUbC promoter

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 (RAB14 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-EF1α-FRT-FLEX-GtACR1-EYFP

    Plasmid

    #114370

    Purpose

    expresses Flpo recombinase-dependent GtACR1

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR1-EYFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCEFLmGFP-Gli2

    Plasmid

    #37672

    Depositor

    Philip Beachy

    Article

    Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP

    Plasmid

    #114369

    Purpose

    expresses Flpo recombinase-dependent GtACR2

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-dfROX

    Plasmid

    #137170

    Purpose

    Lentiviral transfer vector for expression of roGFP2 and HyPer Red

    Depositor

    Shaun McCullough

    Article

    Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

    Insert

    roGFP2-2A-HyPer Red
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAT15482_ABE8e

    Plasmid

    #174120

    Purpose

    Expresses ABE8e with an EF1a promoter

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eABE
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEFL3xHAmGli2

    Plasmid

    #37671

    Depositor

    Philip Beachy

    Article

    Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MCP-ADAR2-DD

    Plasmid

    #170124

    Purpose

    Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWXLd-RIEP-CeNLuc

    Plasmid

    #135934

    Purpose

    Expresses the CeNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Sico-Red

    Plasmid

    #84882

    Purpose

    Cre-inducible shRNA

    Depositor

    Eun Mi Hwang

    Article

    Jung et al Exp Neurobiol. 2016 Jun;25(3):120-9. doi

    Insert

    Sico-Red
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a and CMV

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXPR_124

    Plasmid

    #133461

    Purpose

    EF1a promoter expresses SpyoCas9 and P2A provides EGFP

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    SpyoCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-EF1α-DIO-ReaChR-P2A-dTomato

    Plasmid

    #114368

    Purpose

    expresses a Cre-dependent red light-shifted channelrhodopsin variant

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    ReaChR-P2A-dTomato
    

    Use

    AAV

    Tags

    dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCF221

    Plasmid

    #121669

    Purpose

    U6-sgRNA EF1a-mCherry

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pWXLd-RIEP-GpNLuc

    Plasmid

    #135935

    Purpose

    Expresses the GpNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RAB14 S25N

    Plasmid

    #61495

    Purpose

    to overexpress dominant negative RAB14. Vector includes GFP driven by hUbC promoter

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 (RAB14 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S25N, inactive form

    Promoter

    EF1a

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

26. RAB14 Q70L

    Plasmid

    #61494

    Purpose

    to overexpress constitutively active RAB14. Vector includes GFP driven by hUbC promoter

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 (RAB14 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Q70L, active form

    Promoter

    EF1a

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pAAV-CBh-DIO-CB2-EGFP

    Plasmid

    #91946

    Purpose

    Expression of mouse CB2 cannabinoid receptors and eGFP in the presence of Cre

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Inserts

    EGFP
    type-2 cannabinoid receptor
    

    Use

    AAV

    Tags

    FLAG and None

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. sgRNA(MS2)_zeo insert c-MYC

    Plasmid

    #164636

    Purpose

    sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64

    Depositor

    Günter Schneider

    Insert

    target sequence for c-Myc activation (MYC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ptwB

    Plasmid

    #48735

    Purpose

    Tandem dual tomato plasmid

    Depositor

    Roger Tsien, John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    Tandem dual tomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWXLd-RIEP-OgNLuc

    Plasmid

    #135936

    Purpose

    Expresses the OgNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV.pCry1(408).DIO.Luciferase

    Plasmid

    #192623

    Purpose

    Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

    Depositor

    Michael Hastings

    Article

    Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

    Insert

    luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAID-CMV linearizing in pX330

    Plasmid

    #140609

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-CMV

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEF:iCas9

    Plasmid

    #138267

    Purpose

    Expression of mTN3-GGS6-dCas9 (iCas9) in human.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mTN3-GGS6-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cas9-D10A, H840A

    Promoter

    Ef1a

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEF‐PIK3IP1

    Plasmid

    #49214

    Purpose

    expresses PIK3IP1 in mammalian cells

    Depositor

    Lawrence Kane

    Article

    DeFrances et al Eur J Immunol. 2012 Oct;42(10):2754-9. d

    Insert

    PIK3IP1 (Pik3ip1 Mouse)
    

    Use

    Tags

    6X-his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pCF713

    Plasmid

    #121665

    Purpose

    U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. IDP431

    Plasmid

    #86999

    Purpose

    plasmid for cell culture expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Insert

    CLIP-Nrx1B-4xλN(i)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. IDP455

    Plasmid

    #87001

    Purpose

    plasmid for cell culture expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Insert

    barcode landing site Gfp-4xboxB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDL0_033

    Plasmid

    #210755

    Purpose

    PU_Zea mays EF1a promoter

    Depositor

    Dmitri Nusinow

    Article

    Lee et al bioRxiv 2025.06.20.660755

    Insert

    Zea Mays Elongation factor 1A promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MCP-YPet

    Plasmid

    #215747

    Purpose

    Expresses MS2 coat protein (MCP) fused to YPet

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. IDP254

    Plasmid

    #87004

    Purpose

    plasmid for cell culture expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Insert

    postCLAP
    

    Use

    Synthetic Biology

    Tags

    spGFP (1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. IDP253

    Plasmid

    #87003

    Purpose

    plasmid for cell culture expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Insert

    preCLAP
    

    Use

    Synthetic Biology

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. IDP379

    Plasmid

    #87006

    Purpose

    plasmid for cell culture expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Insert

    postLib-mRFP-4xboxB
    

    Use

    Synthetic Biology

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. IDP378

    Plasmid

    #87005

    Purpose

    plasmid for cell culture expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Insert

    preLib-bfp-4xboxB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. HiFi Cas9

    Plasmid

    #188490

    Purpose

    Expresses FLAG tagged HiFi Cas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    EF1a

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. SaCas9

    Plasmid

    #188494

    Purpose

    Expresses FLAG tagged SaCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. AsCas12a

    Plasmid

    #188492

    Purpose

    Expresses FLAG tagged AsCas12a

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    David Root, John Doench, William Hahn

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. Lenti-miniTurbo-NLS-Blast

    Plasmid

    #232347

    Purpose

    Lentiviral control vector expressing miniTurboNLS without dCas9

    Depositor

    Ali Shilatifard

    Article

    Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

    Insert

    miniTurbo
    

    Use

    Lentiviral

    Tags

    3xHA and 3xSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLX_311-Cas9

    Plasmid

    #96924

    Purpose

    for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

    Depositor

    David Root, John Doench, William Hahn

    Article

    Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    3xFlag and V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pRDA_118

    Plasmid

    #133459

    Purpose

    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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